Self-seeding microwell chip for the isolation and characterization of single cells

Joost F. Swennenhuis, Arjan G.J. Tibbe, Michiel Stevens, Madhumohan R. Katika, Joost Van Dalum, Hien Duy Tong, C.J.M. Van Rijn, Leon W.M.M. Terstappen

Research output: Contribution to journalArticleAcademicpeer-review

52 Citations (Scopus)

Abstract

A self-seeding microwell chip is introduced for the isolation and interrogation of single cells. A cell suspension is transferred to a microwell chip containing 6400 microwells, each microwell with a single 5 μm pore in the bottom. The fluid enters the microwell and drags a cell onto the pore. After a cell has landed onto the pore, it will stop the fluid flow through this microwell. The remaining fluid and cells will be diverted to the next available microwell. This results in a fast and efficient distribution of single cells in individual microwells. After identification by fluorescence microscopy, the cells of interest are isolated from the microwell by punching the bottom together with the cell. The overall single cell recovery of seeding followed by isolation of the single cell, is >70% with a specificity of 100% as confirmed by the genetic make-up of the isolated cells.

Original languageEnglish
Pages (from-to)3039-3046
JournalLab on a Chip
Volume15
Issue number14
DOIs
Publication statusPublished - 2015

Fingerprint Dive into the research topics of 'Self-seeding microwell chip for the isolation and characterization of single cells'. Together they form a unique fingerprint.

Cite this