Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address

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    47 Citations (Scopus)

    Abstract

    The high-throughput deposition of recombinant proteins on chips, beads or biosensor devices would be greatly facilitated by the implementation of self-assembly concepts. DNA-directed immobilization via conjugation of proteins to an oligonucleotide would be preeminently suited for this purpose. Here, we present a unique method to attach a single DNA address to proteins in one step during the purification from the E. coli lysate by fusion to human O6-alkylguanine-DNA-alkyltransferase (SNAP-tagTM) and the Avitag. Use of the conjugates in converting a DNA chip into a protein chip by self assembly is demonstrated.
    Original languageEnglish
    Pages (from-to)2650-2655
    JournalProteomics
    Volume6
    Issue number9
    DOIs
    Publication statusPublished - 2006

    Keywords

    • molecules in-vivo
    • o-6-alkylguanine-dna alkyltransferase
    • directed immobilization
    • microarrays
    • technology
    • efficient
    • ligation
    • vitro
    • hagt

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