Selective ligninolysis of wheat straw and wood chips by the white-rot fungus Lentinula edodes and its influence on in vitro rumen degradability

S.J.A. van Kuijk, José C. del Rio, Jorge Rencoret, Ana Gutiérrez, A.S.M. Sonnenberg, J.J.P. Baars, W.H. Hendriks, J.W. Cone

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

Background: The present work investigated the influence of lignin content and composition in the fungal
treatment of lignocellulosic biomass in order to improve rumen degradability. Wheat straw and wood chips,
differing in lignin composition, were treated with Lentinula edodes for 0, 2, 4, 8 and 12 wk and the changes
occurring during fungal degradation were analyzed using pyrolysis-gas chromatography-mass spectrometry
and detergent fiber analysis.
Results: L. edodes preferentially degraded lignin, with only limited cellulose degradation, in wheat straw and
wood chips, leaving a substrate enriched in cellulose. Syringyl (S)-lignin units were preferentially degraded than
guaiacyl (G)-lignin units, resulting in a decreased S/G ratio. A decreasing S/G ratio (wheat straw: r = −0.72, wood
chips: r = −0.75) and selective lignin degradation (wheat straw: r = −0.69, wood chips: r = −0.88) were correlated
with in vitro gas production (IVGP), a good indicator for rumen degradability.
Conclusions: L. edodes treatment increased the IVGP of wheat straw and wood chips. Effects on IVGP were
similar for wheat straw and wood chips indicating that lignin content and 3D-structure of cell walls influence
in vitro rumen degradability more than lignin composition.
Keywords: Fungal treatment, In vitro rumen degradability, Lignocellulosic biomass, Py-GC/MS
LanguageEnglish
Article number55
Number of pages14
JournalJournal of Animal Science and Biotechnology
Volume7
DOIs
Publication statusPublished - 2016

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Shiitake Mushrooms
Lentinula edodes
white-rot fungi
wood chips
Lignin
Rumen
Straw
wheat straw
Fungi
Triticum
lignin
rumen
Wood
gas production (biological)
Gases
Cellulose
Degradation
Biomass
degradation
cellulose

Cite this

@article{c10b99d550964b10911ae27ecbe11a05,
title = "Selective ligninolysis of wheat straw and wood chips by the white-rot fungus Lentinula edodes and its influence on in vitro rumen degradability",
abstract = "Background: The present work investigated the influence of lignin content and composition in the fungaltreatment of lignocellulosic biomass in order to improve rumen degradability. Wheat straw and wood chips,differing in lignin composition, were treated with Lentinula edodes for 0, 2, 4, 8 and 12 wk and the changesoccurring during fungal degradation were analyzed using pyrolysis-gas chromatography-mass spectrometryand detergent fiber analysis.Results: L. edodes preferentially degraded lignin, with only limited cellulose degradation, in wheat straw andwood chips, leaving a substrate enriched in cellulose. Syringyl (S)-lignin units were preferentially degraded thanguaiacyl (G)-lignin units, resulting in a decreased S/G ratio. A decreasing S/G ratio (wheat straw: r = −0.72, woodchips: r = −0.75) and selective lignin degradation (wheat straw: r = −0.69, wood chips: r = −0.88) were correlatedwith in vitro gas production (IVGP), a good indicator for rumen degradability.Conclusions: L. edodes treatment increased the IVGP of wheat straw and wood chips. Effects on IVGP weresimilar for wheat straw and wood chips indicating that lignin content and 3D-structure of cell walls influencein vitro rumen degradability more than lignin composition.Keywords: Fungal treatment, In vitro rumen degradability, Lignocellulosic biomass, Py-GC/MS",
author = "{van Kuijk}, S.J.A. and {del Rio}, {Jos{\'e} C.} and Jorge Rencoret and Ana Guti{\'e}rrez and A.S.M. Sonnenberg and J.J.P. Baars and W.H. Hendriks and J.W. Cone",
year = "2016",
doi = "10.1186/s40104-016-0110-z",
language = "English",
volume = "7",
journal = "Journal of Animal Science and Biotechnology",
issn = "1674-9782",
publisher = "BioMed Central",

}

Selective ligninolysis of wheat straw and wood chips by the white-rot fungus Lentinula edodes and its influence on in vitro rumen degradability. / van Kuijk, S.J.A.; del Rio, José C.; Rencoret, Jorge; Gutiérrez, Ana; Sonnenberg, A.S.M.; Baars, J.J.P.; Hendriks, W.H.; Cone, J.W.

In: Journal of Animal Science and Biotechnology, Vol. 7, 55, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Selective ligninolysis of wheat straw and wood chips by the white-rot fungus Lentinula edodes and its influence on in vitro rumen degradability

AU - van Kuijk, S.J.A.

AU - del Rio, José C.

AU - Rencoret, Jorge

AU - Gutiérrez, Ana

AU - Sonnenberg, A.S.M.

AU - Baars, J.J.P.

AU - Hendriks, W.H.

AU - Cone, J.W.

PY - 2016

Y1 - 2016

N2 - Background: The present work investigated the influence of lignin content and composition in the fungaltreatment of lignocellulosic biomass in order to improve rumen degradability. Wheat straw and wood chips,differing in lignin composition, were treated with Lentinula edodes for 0, 2, 4, 8 and 12 wk and the changesoccurring during fungal degradation were analyzed using pyrolysis-gas chromatography-mass spectrometryand detergent fiber analysis.Results: L. edodes preferentially degraded lignin, with only limited cellulose degradation, in wheat straw andwood chips, leaving a substrate enriched in cellulose. Syringyl (S)-lignin units were preferentially degraded thanguaiacyl (G)-lignin units, resulting in a decreased S/G ratio. A decreasing S/G ratio (wheat straw: r = −0.72, woodchips: r = −0.75) and selective lignin degradation (wheat straw: r = −0.69, wood chips: r = −0.88) were correlatedwith in vitro gas production (IVGP), a good indicator for rumen degradability.Conclusions: L. edodes treatment increased the IVGP of wheat straw and wood chips. Effects on IVGP weresimilar for wheat straw and wood chips indicating that lignin content and 3D-structure of cell walls influencein vitro rumen degradability more than lignin composition.Keywords: Fungal treatment, In vitro rumen degradability, Lignocellulosic biomass, Py-GC/MS

AB - Background: The present work investigated the influence of lignin content and composition in the fungaltreatment of lignocellulosic biomass in order to improve rumen degradability. Wheat straw and wood chips,differing in lignin composition, were treated with Lentinula edodes for 0, 2, 4, 8 and 12 wk and the changesoccurring during fungal degradation were analyzed using pyrolysis-gas chromatography-mass spectrometryand detergent fiber analysis.Results: L. edodes preferentially degraded lignin, with only limited cellulose degradation, in wheat straw andwood chips, leaving a substrate enriched in cellulose. Syringyl (S)-lignin units were preferentially degraded thanguaiacyl (G)-lignin units, resulting in a decreased S/G ratio. A decreasing S/G ratio (wheat straw: r = −0.72, woodchips: r = −0.75) and selective lignin degradation (wheat straw: r = −0.69, wood chips: r = −0.88) were correlatedwith in vitro gas production (IVGP), a good indicator for rumen degradability.Conclusions: L. edodes treatment increased the IVGP of wheat straw and wood chips. Effects on IVGP weresimilar for wheat straw and wood chips indicating that lignin content and 3D-structure of cell walls influencein vitro rumen degradability more than lignin composition.Keywords: Fungal treatment, In vitro rumen degradability, Lignocellulosic biomass, Py-GC/MS

U2 - 10.1186/s40104-016-0110-z

DO - 10.1186/s40104-016-0110-z

M3 - Article

VL - 7

JO - Journal of Animal Science and Biotechnology

T2 - Journal of Animal Science and Biotechnology

JF - Journal of Animal Science and Biotechnology

SN - 1674-9782

M1 - 55

ER -