Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment

P.R. Postma, G. Pataro*, M. Capitoli, M.J. Barbosa, R.H. Wijffels, M.H.M. Eppink, G. Olivieri, G. Ferrari

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

81 Citations (Scopus)

Abstract

The synergistic effect of temperature (25-65°C) and total specific energy input (0.55-1.11kWhkgDW -1) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55°C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25-39% carbohydrates and 3-5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55°C. Above 55°C spontaneous cell lysis occurred without PEF. Combined PEF-temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40-45% protein, 48-58% carbohydrates).

Original languageEnglish
Pages (from-to)80-88
JournalBioresource Technology
Volume203
DOIs
Publication statusPublished - 1 Mar 2016

Keywords

  • Bead mill
  • Microalgae
  • Mild cascade biorefinery
  • Pulsed electric field
  • Temperature

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