Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment

P.R. Postma; G. Pataro; M. Capitoli; M.J. Barbosa; R.H. Wijffels; M.H.M. Eppink; G. Olivieri; G. Ferrari

doi:10.1016/j.biortech.2015.12.012

Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment

P.R. Postma, G. Pataro^*, M. Capitoli, M.J. Barbosa, R.H. Wijffels, M.H.M. Eppink, G. Olivieri, G. Ferrari

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

157 Citations (Scopus)

Abstract

The synergistic effect of temperature (25-65°C) and total specific energy input (0.55-1.11kWhkg_DW ^-1) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55°C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25-39% carbohydrates and 3-5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55°C. Above 55°C spontaneous cell lysis occurred without PEF. Combined PEF-temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40-45% protein, 48-58% carbohydrates).

Original language	English
Pages (from-to)	80-88
Journal	Bioresource Technology
Volume	203
DOIs	https://doi.org/10.1016/j.biortech.2015.12.012
Publication status	Published - 1 Mar 2016

Keywords

Bead mill
Microalgae
Mild cascade biorefinery
Pulsed electric field
Temperature

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10.1016/j.biortech.2015.12.012

Cite this

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title = "Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment",

abstract = "The synergistic effect of temperature (25-65°C) and total specific energy input (0.55-1.11kWhkgDW -1) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55°C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25-39% carbohydrates and 3-5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55°C. Above 55°C spontaneous cell lysis occurred without PEF. Combined PEF-temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40-45% protein, 48-58% carbohydrates).",

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T1 - Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment

AU - Postma, P.R.

AU - Pataro, G.

AU - Capitoli, M.

AU - Barbosa, M.J.

AU - Wijffels, R.H.

AU - Eppink, M.H.M.

AU - Olivieri, G.

AU - Ferrari, G.

PY - 2016/3/1

Y1 - 2016/3/1

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AB - The synergistic effect of temperature (25-65°C) and total specific energy input (0.55-1.11kWhkgDW -1) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55°C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25-39% carbohydrates and 3-5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55°C. Above 55°C spontaneous cell lysis occurred without PEF. Combined PEF-temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40-45% protein, 48-58% carbohydrates).

KW - Bead mill

KW - Microalgae

KW - Mild cascade biorefinery

KW - Pulsed electric field

KW - Temperature

U2 - 10.1016/j.biortech.2015.12.012

DO - 10.1016/j.biortech.2015.12.012

M3 - Article

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SN - 0960-8524

VL - 203

SP - 80

EP - 88

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment

Abstract

Keywords

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