Selective enrichment of Geobacter sulfurreducens from anaerobic granular sludge with quinones as terminal electron acceptors

F.J. Cervantes-Carillo, T. Duong Dac, A.E. Ivanova, K. de Roest, A.D.L. Akkermans, G. Lettinga, J.A. Field

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

A quinone-respiring, enrichment culture derived from methanogenic granular sludge was phylogenetically characterized by using a combined cloning-denaturing gradient gel electrophoresis (DGGE) method, which revealed that the consortium developed was dominated by a single microorganism: 97% related, in a sequence of 1520 base pairs, to Geobacter sulfurreducens. The enrichment culture could grow with acetate, formate or H-2 when humic acids, the humic model compound, anthraquinone-2,6-disulfonate (AQDS), or chelated Fe(III) was provided as a terminal electron acceptor. The occurrence of a humic acid- or quinone-respiring microorganism in the microbial community of a wastewater treatment system suggests that this type of microorganisms may play a potential role in anaerobic bioreactors treating humus-containing wastewaters.
Original languageEnglish
Pages (from-to)39-45
JournalBiotechnology Letters
Volume25
Issue number1
DOIs
Publication statusPublished - 2003

Keywords

  • humic substances
  • redox mediators
  • reduction
  • respiration
  • bacteria
  • recovery

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