Selective carbohydrate utilization by lactobacilli and bifidobacteria

D. Watson, M. O'Connell Motherway, M.H.C. Schoterman, R.J.J. van Neerven, A. Nauta, D. van Sinderen

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103 Citations (Scopus)

Abstract

Aim - To evaluate the ability of specific carbohydrates, including commercially available products, to support the growth of representatives of two well-known groups of gut commensals, namely lactobacilli and bifidobacteria. Methods and Results - Sixty-eight bacterial strains, representing 29 human-derived lactobacilli and 39 bifidobacteria (both human- and animal-derived), were tested for their ability to metabolize 10 different carbohydrates. Analysis of growth and metabolic activity was performed using a combination of diagnostic parameters, such as final OD600, final pH, fermentation end products and growth rate. Conclusions - The data assembled in this study provide significant complementary and comparative information on the growth-promoting properties of a range of carbohydrates, while also investigating interspecies differences between lactobacilli and/or bifidobacteria with regard to their carbohydrate utilization abilities. Galacto-oligosaccharides (GOS) and lactulose were shown to support the most favourable growth characteristics, whereas relatively poor growth of lactobacilli and bifidobacteria was observed on inulin, maltodextrin and polydextrose. GOS/inulin (9 : 1) and fructo-oligosaccharides (FOS)/inulin mixtures supported mostly similar growth abilities to those obtained for GOS and FOS, respectively. Microbial consumption of GOS, as determined by high-performance anion-exchange chromatography with pulsed amperometric detection, was evident for both lactobacilli and bifidobacteria.
Original languageEnglish
Pages (from-to)1132-1146
JournalJournal of Applied Microbiology
Volume114
Issue number4
DOIs
Publication statusPublished - 2013

Keywords

  • galacto-oligosaccharides
  • comparative genomics
  • beta-galactosidase
  • gut microbiome
  • prebiotics
  • growth
  • inulin
  • milk
  • oligofructose
  • modulation

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  • Cite this

    Watson, D., O'Connell Motherway, M., Schoterman, M. H. C., van Neerven, R. J. J., Nauta, A., & van Sinderen, D. (2013). Selective carbohydrate utilization by lactobacilli and bifidobacteria. Journal of Applied Microbiology, 114(4), 1132-1146. https://doi.org/10.1111/jam.12105