Selective blockade of Phosphodiesterases Type 2, 5 and 9 results in cGMP accumulation in Retinal Pigment Epithelium Cells

R. Diederen, E.C. La Heij, M. Markerink-van Ittersum, A. Kijlstra, F. Hendrikse, J. de Vente

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

Aim: To investigate which phosphodiesterase (PDE) is involved in regulating cyclic 3'5' guanosine monophosphate breakdown in retinal pigment epithelium (RPE) cells. Methods: cGMP content in the cultured RPE cells (D407 cell line) was evaluated by immunocytochemistry in the presence of non-selective or isoform-selective PDE inhibitors in combination with the particulate guanylyl cyclase stimulator atrial natriuretic peptide (ANP) or the soluble guanylyl cyclase stimulator sodium nitroprusside (SNP). mRNA expression of PDE2, PDE5 and PDE9 was studied in cultured human RPE cells and rat RPE cell layers using non-radioactive in situ hybridisation. Results: In the absence of PDE inhibitors, cGMP levels in cultured RPE cells are very low. cGMP accumulation was readily detected in cultured human RPE cells after incubation with Bay60–7550 as a selective PDE2 inhibitor, sildenafil as a selective PDE5 inhibitor or Sch51866 as a selective PDE9 inhibitor. In the presence of PDE inhibition, cGMP content increased markedly after stimulation of the particulate guanylyl cyclase. mRNA of PDE2,PDE5 and PDE9 was detected in all cultured human RPE cells and also in rat RPE cell layers
Original languageEnglish
Pages (from-to)379-384
JournalBritish Journal of Ophthalmology
Volume91
Issue number3
DOIs
Publication statusPublished - 2007

Keywords

  • nucleotide phosphodiesterases
  • subretinal fluid
  • cgmp phosphodiesterase
  • rat-brain
  • sildenafil
  • cloning
  • pde10a
  • camp
  • amp

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