Abstract
BACKGROUND: Microalgal biomass is generally used to produce a single product instead of valorizing all of the cellular components. The biomass production and downstream processes are too expensive if only one product is valorized. A new approach was proposed for the simultaneous and selective partitioning of pigments and proteins from disrupted Neochloris oleoabundans cultivated under saline and freshwater conditions. RESULTS: An aqueous two-phase system composed of polyethylene glycol and cholinium dihydrogen phosphate selectively separated microalgal pigments from microalgal proteins. 97.3 ± 1.0% of lutein and 51.6 ± 2.3% of chlorophyll were recovered in the polymer-rich phase. Simultaneously, up to 92.2 ± 2.0% of proteins were recovered in a third phase (interface) in between the aqueous phases (interface). The recovered proteins, including Rubisco with a molecular weight of ∼560 kDa, seem to be intact and pigments did not suffer degradation, demonstrating the mildness of this system for fractionating microalgal biomolecules. CONCLUSION: The ability of aqueous two-phase systems (ATPSs) to simultaneously and efficiently fractionate different biomolecules in a mild manner from disrupted microalgae is demonstrated. This is an important step towards the development of a multiproduct microalgae biorefinery.
| Original language | English |
|---|---|
| Pages (from-to) | 2774-2783 |
| Journal | Journal of Chemical Technology and Biotechnology |
| Volume | 93 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 1 Sep 2018 |
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Keywords
- aqueous two-phase systems
- cholinium-based ionic liquids
- microalgae biorefinery
- pigments
- proteins
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Selective and mild fractionation of microalgal proteins and pigments using aqueous two-phase systems. / Suarez Ruiz, Catalina A.; Emmery, Daniel P.; Wijffels, Rene H.; Eppink, Michel H.M.; van den Berg, Corjan.
In: Journal of Chemical Technology and Biotechnology, Vol. 93, No. 9, 01.09.2018, p. 2774-2783.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Selective and mild fractionation of microalgal proteins and pigments using aqueous two-phase systems
AU - Suarez Ruiz, Catalina A.
AU - Emmery, Daniel P.
AU - Wijffels, Rene H.
AU - Eppink, Michel H.M.
AU - van den Berg, Corjan
PY - 2018/9/1
Y1 - 2018/9/1
N2 - BACKGROUND: Microalgal biomass is generally used to produce a single product instead of valorizing all of the cellular components. The biomass production and downstream processes are too expensive if only one product is valorized. A new approach was proposed for the simultaneous and selective partitioning of pigments and proteins from disrupted Neochloris oleoabundans cultivated under saline and freshwater conditions. RESULTS: An aqueous two-phase system composed of polyethylene glycol and cholinium dihydrogen phosphate selectively separated microalgal pigments from microalgal proteins. 97.3 ± 1.0% of lutein and 51.6 ± 2.3% of chlorophyll were recovered in the polymer-rich phase. Simultaneously, up to 92.2 ± 2.0% of proteins were recovered in a third phase (interface) in between the aqueous phases (interface). The recovered proteins, including Rubisco with a molecular weight of ∼560 kDa, seem to be intact and pigments did not suffer degradation, demonstrating the mildness of this system for fractionating microalgal biomolecules. CONCLUSION: The ability of aqueous two-phase systems (ATPSs) to simultaneously and efficiently fractionate different biomolecules in a mild manner from disrupted microalgae is demonstrated. This is an important step towards the development of a multiproduct microalgae biorefinery.
AB - BACKGROUND: Microalgal biomass is generally used to produce a single product instead of valorizing all of the cellular components. The biomass production and downstream processes are too expensive if only one product is valorized. A new approach was proposed for the simultaneous and selective partitioning of pigments and proteins from disrupted Neochloris oleoabundans cultivated under saline and freshwater conditions. RESULTS: An aqueous two-phase system composed of polyethylene glycol and cholinium dihydrogen phosphate selectively separated microalgal pigments from microalgal proteins. 97.3 ± 1.0% of lutein and 51.6 ± 2.3% of chlorophyll were recovered in the polymer-rich phase. Simultaneously, up to 92.2 ± 2.0% of proteins were recovered in a third phase (interface) in between the aqueous phases (interface). The recovered proteins, including Rubisco with a molecular weight of ∼560 kDa, seem to be intact and pigments did not suffer degradation, demonstrating the mildness of this system for fractionating microalgal biomolecules. CONCLUSION: The ability of aqueous two-phase systems (ATPSs) to simultaneously and efficiently fractionate different biomolecules in a mild manner from disrupted microalgae is demonstrated. This is an important step towards the development of a multiproduct microalgae biorefinery.
KW - aqueous two-phase systems
KW - cholinium-based ionic liquids
KW - microalgae biorefinery
KW - pigments
KW - proteins
U2 - 10.1002/jctb.5711
DO - 10.1002/jctb.5711
M3 - Article
VL - 93
SP - 2774
EP - 2783
JO - Journal of Chemical Technology and Biotechnology
JF - Journal of Chemical Technology and Biotechnology
SN - 0268-2575
IS - 9
ER -