Selective and mild fractionation of microalgal proteins and pigments using aqueous two-phase systems

Catalina A. Suarez Ruiz*, Daniel P. Emmery, Rene H. Wijffels, Michel H.M. Eppink, Corjan van den Berg

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

BACKGROUND: Microalgal biomass is generally used to produce a single product instead of valorizing all of the cellular components. The biomass production and downstream processes are too expensive if only one product is valorized. A new approach was proposed for the simultaneous and selective partitioning of pigments and proteins from disrupted Neochloris oleoabundans cultivated under saline and freshwater conditions. RESULTS: An aqueous two-phase system composed of polyethylene glycol and cholinium dihydrogen phosphate selectively separated microalgal pigments from microalgal proteins. 97.3 ± 1.0% of lutein and 51.6 ± 2.3% of chlorophyll were recovered in the polymer-rich phase. Simultaneously, up to 92.2 ± 2.0% of proteins were recovered in a third phase (interface) in between the aqueous phases (interface). The recovered proteins, including Rubisco with a molecular weight of ∼560 kDa, seem to be intact and pigments did not suffer degradation, demonstrating the mildness of this system for fractionating microalgal biomolecules. CONCLUSION: The ability of aqueous two-phase systems (ATPSs) to simultaneously and efficiently fractionate different biomolecules in a mild manner from disrupted microalgae is demonstrated. This is an important step towards the development of a multiproduct microalgae biorefinery.

Original languageEnglish
Pages (from-to)2774-2783
JournalJournal of Chemical Technology and Biotechnology
Volume93
Issue number9
DOIs
Publication statusPublished - 1 Sep 2018

Fingerprint

Fractionation
Pigments
pigment
fractionation
Proteins
Microalgae
Phase interfaces
protein
Biomolecules
Biomass
Ribulose-Bisphosphate Carboxylase
Lutein
biomass
Chlorophyll
Fresh Water
Polyethylene glycols
Polymers
chlorophyll
Phosphates
partitioning

Keywords

  • aqueous two-phase systems
  • cholinium-based ionic liquids
  • microalgae biorefinery
  • pigments
  • proteins

Cite this

@article{09509399ad3b4606ba20351e3966e4d1,
title = "Selective and mild fractionation of microalgal proteins and pigments using aqueous two-phase systems",
abstract = "BACKGROUND: Microalgal biomass is generally used to produce a single product instead of valorizing all of the cellular components. The biomass production and downstream processes are too expensive if only one product is valorized. A new approach was proposed for the simultaneous and selective partitioning of pigments and proteins from disrupted Neochloris oleoabundans cultivated under saline and freshwater conditions. RESULTS: An aqueous two-phase system composed of polyethylene glycol and cholinium dihydrogen phosphate selectively separated microalgal pigments from microalgal proteins. 97.3 ± 1.0{\%} of lutein and 51.6 ± 2.3{\%} of chlorophyll were recovered in the polymer-rich phase. Simultaneously, up to 92.2 ± 2.0{\%} of proteins were recovered in a third phase (interface) in between the aqueous phases (interface). The recovered proteins, including Rubisco with a molecular weight of ∼560 kDa, seem to be intact and pigments did not suffer degradation, demonstrating the mildness of this system for fractionating microalgal biomolecules. CONCLUSION: The ability of aqueous two-phase systems (ATPSs) to simultaneously and efficiently fractionate different biomolecules in a mild manner from disrupted microalgae is demonstrated. This is an important step towards the development of a multiproduct microalgae biorefinery.",
keywords = "aqueous two-phase systems, cholinium-based ionic liquids, microalgae biorefinery, pigments, proteins",
author = "{Suarez Ruiz}, {Catalina A.} and Emmery, {Daniel P.} and Wijffels, {Rene H.} and Eppink, {Michel H.M.} and {van den Berg}, Corjan",
year = "2018",
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Selective and mild fractionation of microalgal proteins and pigments using aqueous two-phase systems. / Suarez Ruiz, Catalina A.; Emmery, Daniel P.; Wijffels, Rene H.; Eppink, Michel H.M.; van den Berg, Corjan.

In: Journal of Chemical Technology and Biotechnology, Vol. 93, No. 9, 01.09.2018, p. 2774-2783.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Selective and mild fractionation of microalgal proteins and pigments using aqueous two-phase systems

AU - Suarez Ruiz, Catalina A.

AU - Emmery, Daniel P.

AU - Wijffels, Rene H.

AU - Eppink, Michel H.M.

AU - van den Berg, Corjan

PY - 2018/9/1

Y1 - 2018/9/1

N2 - BACKGROUND: Microalgal biomass is generally used to produce a single product instead of valorizing all of the cellular components. The biomass production and downstream processes are too expensive if only one product is valorized. A new approach was proposed for the simultaneous and selective partitioning of pigments and proteins from disrupted Neochloris oleoabundans cultivated under saline and freshwater conditions. RESULTS: An aqueous two-phase system composed of polyethylene glycol and cholinium dihydrogen phosphate selectively separated microalgal pigments from microalgal proteins. 97.3 ± 1.0% of lutein and 51.6 ± 2.3% of chlorophyll were recovered in the polymer-rich phase. Simultaneously, up to 92.2 ± 2.0% of proteins were recovered in a third phase (interface) in between the aqueous phases (interface). The recovered proteins, including Rubisco with a molecular weight of ∼560 kDa, seem to be intact and pigments did not suffer degradation, demonstrating the mildness of this system for fractionating microalgal biomolecules. CONCLUSION: The ability of aqueous two-phase systems (ATPSs) to simultaneously and efficiently fractionate different biomolecules in a mild manner from disrupted microalgae is demonstrated. This is an important step towards the development of a multiproduct microalgae biorefinery.

AB - BACKGROUND: Microalgal biomass is generally used to produce a single product instead of valorizing all of the cellular components. The biomass production and downstream processes are too expensive if only one product is valorized. A new approach was proposed for the simultaneous and selective partitioning of pigments and proteins from disrupted Neochloris oleoabundans cultivated under saline and freshwater conditions. RESULTS: An aqueous two-phase system composed of polyethylene glycol and cholinium dihydrogen phosphate selectively separated microalgal pigments from microalgal proteins. 97.3 ± 1.0% of lutein and 51.6 ± 2.3% of chlorophyll were recovered in the polymer-rich phase. Simultaneously, up to 92.2 ± 2.0% of proteins were recovered in a third phase (interface) in between the aqueous phases (interface). The recovered proteins, including Rubisco with a molecular weight of ∼560 kDa, seem to be intact and pigments did not suffer degradation, demonstrating the mildness of this system for fractionating microalgal biomolecules. CONCLUSION: The ability of aqueous two-phase systems (ATPSs) to simultaneously and efficiently fractionate different biomolecules in a mild manner from disrupted microalgae is demonstrated. This is an important step towards the development of a multiproduct microalgae biorefinery.

KW - aqueous two-phase systems

KW - cholinium-based ionic liquids

KW - microalgae biorefinery

KW - pigments

KW - proteins

U2 - 10.1002/jctb.5711

DO - 10.1002/jctb.5711

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EP - 2783

JO - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

SN - 0268-2575

IS - 9

ER -