Selection of chemically defined media for CHO cell fed-batch culture processes

X. Pan*, M. Streefland, C. Dalm, R.H. Wijffels, D.E. Martens

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

39 Citations (Scopus)


Two CHO cell clones derived from the same parental CHOBC cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures.
Higher amino acid feeding did not always lead to igher mAb production. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and roduction rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B.
The BC-P clone fed by Actifeed A/B showed a threefold cell volume increase and an increase of the specific consumption rate of glucose in the stationary
phase. Since feeding was based on glucose this resulted in accumulation of amino acids for this feed, while this did not occur for the poorer feed (EFA/B).
The same feed also led to an increase of cell size for the BC-G clone, but to a lesser extent.
Original languageEnglish
Pages (from-to)39-56
Issue number1
Publication statusPublished - 2017


  • Antibody production
  • Cell culture medium
  • Chinese hamster ovary (CHO) cell
  • Fed-batch
  • Metabolite profile
  • Phase transition


Dive into the research topics of 'Selection of chemically defined media for CHO cell fed-batch culture processes'. Together they form a unique fingerprint.

Cite this