Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport

P.J.I. van de Vondervoort; M.J.L. de Groot; G.J.G. Ruijter; J. Visser

doi:10.1007/s00253-006-0527-y

Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport

P.J.I. van de Vondervoort, M.J.L. de Groot, G.J.G. Ruijter, J. Visser

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

Aspergillus niger is known for its biotechnological applications, such as the use of xylanase enzyme for the degradation of hemicellulose. Depending on culture conditions, several polyols may also be accumulated, such as xylitol during D-xylose oxidation. Also during industrial fermentation of xylose for the production of fuel ethanol by recombinant yeast, xylitol is a by-product. We studied xylitol metabolism by isolating mutants that have impaired xylitol-mediated repression. Genetic and biochemical characterisation revealed that one of these mutants was affected not only in xylitol-mediated carbon repression, but also had impaired xylitol transport

Original language	English
Pages (from-to)	881-886
Journal	Applied Microbiology and Biotechnology
Volume	73
Issue number	4
DOIs	https://doi.org/10.1007/s00253-006-0527-y
Publication status	Published - 2006

Keywords

metabolic-control analysis
saccharomyces-cerevisiae
l-arabinose
xylose catabolism
fermentation
xylan
construction
degradation
nidulans
genes

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title = "Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport",

abstract = "Aspergillus niger is known for its biotechnological applications, such as the use of xylanase enzyme for the degradation of hemicellulose. Depending on culture conditions, several polyols may also be accumulated, such as xylitol during D-xylose oxidation. Also during industrial fermentation of xylose for the production of fuel ethanol by recombinant yeast, xylitol is a by-product. We studied xylitol metabolism by isolating mutants that have impaired xylitol-mediated repression. Genetic and biochemical characterisation revealed that one of these mutants was affected not only in xylitol-mediated carbon repression, but also had impaired xylitol transport",

keywords = "metabolic-control analysis, saccharomyces-cerevisiae, l-arabinose, xylose catabolism, fermentation, xylan, construction, degradation, nidulans, genes",

author = "{van de Vondervoort}, P.J.I. and {de Groot}, M.J.L. and G.J.G. Ruijter and J. Visser",

year = "2006",

doi = "10.1007/s00253-006-0527-y",

language = "English",

volume = "73",

pages = "881--886",

journal = "Applied Microbiology and Biotechnology",

issn = "0175-7598",

publisher = "Springer Verlag",

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T1 - Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport

AU - van de Vondervoort, P.J.I.

AU - de Groot, M.J.L.

AU - Ruijter, G.J.G.

AU - Visser, J.

PY - 2006

Y1 - 2006

N2 - Aspergillus niger is known for its biotechnological applications, such as the use of xylanase enzyme for the degradation of hemicellulose. Depending on culture conditions, several polyols may also be accumulated, such as xylitol during D-xylose oxidation. Also during industrial fermentation of xylose for the production of fuel ethanol by recombinant yeast, xylitol is a by-product. We studied xylitol metabolism by isolating mutants that have impaired xylitol-mediated repression. Genetic and biochemical characterisation revealed that one of these mutants was affected not only in xylitol-mediated carbon repression, but also had impaired xylitol transport

AB - Aspergillus niger is known for its biotechnological applications, such as the use of xylanase enzyme for the degradation of hemicellulose. Depending on culture conditions, several polyols may also be accumulated, such as xylitol during D-xylose oxidation. Also during industrial fermentation of xylose for the production of fuel ethanol by recombinant yeast, xylitol is a by-product. We studied xylitol metabolism by isolating mutants that have impaired xylitol-mediated repression. Genetic and biochemical characterisation revealed that one of these mutants was affected not only in xylitol-mediated carbon repression, but also had impaired xylitol transport

KW - metabolic-control analysis

KW - saccharomyces-cerevisiae

KW - l-arabinose

KW - xylose catabolism

KW - fermentation

KW - xylan

KW - construction

KW - degradation

KW - nidulans

KW - genes

U2 - 10.1007/s00253-006-0527-y

DO - 10.1007/s00253-006-0527-y

M3 - Article

VL - 73

SP - 881

EP - 886

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 4

ER -

Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport

Abstract

Keywords

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