Selecting microalgae with high lipid productivity and photosynthetic activity under nitrogen starvation

G. Benvenuti, R. Bosma, M. Cuaresma Franco, M.G.J. Janssen, M.J. Barbosa, R.H. Wijffels

Research output: Contribution to journalArticleAcademicpeer-review

42 Citations (Scopus)

Abstract

An economically feasible microalgal lipid industry heavily relies on the selection of suitable strains. Because microalgae lipid content increases under a range of adverse conditions (e.g. nutrient deprivation, high light intensity), photosynthetic activity is usually strongly reduced. As a consequence, lipid productivity rapidly declines overtime, after reaching a maximum within the first days of cultivation. The microalgae Chlorella vulgaris, Chlorococcum littorale, Nannochloropsis oculata, Nannochloropsis sp., Neochloris oleoabundans, Stichococcus bacillaris and Tetraselmis suecica were compared on fatty acid content and productivity, and also on photosynthetic activity under nitrogen (N) starvation. Cultures in N-replete conditions were used as reference. Photosystem II (PSII) maximumefficiency was followed during the experiment, as proxy for the change in photosynthetic activity of the cells. Strains with a high capacity for both lipid accumulation as well as high photosynthetic activity under N starvation exhibited a high lipid productivity over time. Among the tested strains, Nannochloropsis sp. showed highest fatty acid content (45%w/w) and productivity (238 mg L-1 day-1) aswell as PSII maximum efficiency, demonstrating to be the most suitable strain, of those tested, for lipid production. This study highlights that for microalgae, maintaining a high photosynthetic efficiency during stress is the key to maintain high fatty acid productivities overtime and should be considered when selecting strains for microalgal lipid production.
Original languageEnglish
Pages (from-to)1425-1431
JournalJournal of Applied Phycology
Volume27
Issue number4
DOIs
Publication statusPublished - 2015

Fingerprint

microalgae
starvation
lipid
Nannochloropsis
productivity
nitrogen
lipids
fatty acid
photosystem II
Ettlia oleoabundans
Stichococcus
Chlorococcum
fatty acid composition
Tetraselmis suecica
Chlorella vulgaris
light intensity
lipid content
fatty acids
industry
nutrients

Keywords

  • neochloris-oleoabundans
  • parietochloris-incisa
  • marine-phytoplankton
  • biodiesel production
  • nutrient stress
  • fatty-acids
  • light
  • accumulation
  • efficiency
  • biofuels

Cite this

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title = "Selecting microalgae with high lipid productivity and photosynthetic activity under nitrogen starvation",
abstract = "An economically feasible microalgal lipid industry heavily relies on the selection of suitable strains. Because microalgae lipid content increases under a range of adverse conditions (e.g. nutrient deprivation, high light intensity), photosynthetic activity is usually strongly reduced. As a consequence, lipid productivity rapidly declines overtime, after reaching a maximum within the first days of cultivation. The microalgae Chlorella vulgaris, Chlorococcum littorale, Nannochloropsis oculata, Nannochloropsis sp., Neochloris oleoabundans, Stichococcus bacillaris and Tetraselmis suecica were compared on fatty acid content and productivity, and also on photosynthetic activity under nitrogen (N) starvation. Cultures in N-replete conditions were used as reference. Photosystem II (PSII) maximumefficiency was followed during the experiment, as proxy for the change in photosynthetic activity of the cells. Strains with a high capacity for both lipid accumulation as well as high photosynthetic activity under N starvation exhibited a high lipid productivity over time. Among the tested strains, Nannochloropsis sp. showed highest fatty acid content (45{\%}w/w) and productivity (238 mg L-1 day-1) aswell as PSII maximum efficiency, demonstrating to be the most suitable strain, of those tested, for lipid production. This study highlights that for microalgae, maintaining a high photosynthetic efficiency during stress is the key to maintain high fatty acid productivities overtime and should be considered when selecting strains for microalgal lipid production.",
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author = "G. Benvenuti and R. Bosma and {Cuaresma Franco}, M. and M.G.J. Janssen and M.J. Barbosa and R.H. Wijffels",
year = "2015",
doi = "10.1007/s10811-014-0470-8",
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Selecting microalgae with high lipid productivity and photosynthetic activity under nitrogen starvation. / Benvenuti, G.; Bosma, R.; Cuaresma Franco, M.; Janssen, M.G.J.; Barbosa, M.J.; Wijffels, R.H.

In: Journal of Applied Phycology, Vol. 27, No. 4, 2015, p. 1425-1431.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Selecting microalgae with high lipid productivity and photosynthetic activity under nitrogen starvation

AU - Benvenuti, G.

AU - Bosma, R.

AU - Cuaresma Franco, M.

AU - Janssen, M.G.J.

AU - Barbosa, M.J.

AU - Wijffels, R.H.

PY - 2015

Y1 - 2015

N2 - An economically feasible microalgal lipid industry heavily relies on the selection of suitable strains. Because microalgae lipid content increases under a range of adverse conditions (e.g. nutrient deprivation, high light intensity), photosynthetic activity is usually strongly reduced. As a consequence, lipid productivity rapidly declines overtime, after reaching a maximum within the first days of cultivation. The microalgae Chlorella vulgaris, Chlorococcum littorale, Nannochloropsis oculata, Nannochloropsis sp., Neochloris oleoabundans, Stichococcus bacillaris and Tetraselmis suecica were compared on fatty acid content and productivity, and also on photosynthetic activity under nitrogen (N) starvation. Cultures in N-replete conditions were used as reference. Photosystem II (PSII) maximumefficiency was followed during the experiment, as proxy for the change in photosynthetic activity of the cells. Strains with a high capacity for both lipid accumulation as well as high photosynthetic activity under N starvation exhibited a high lipid productivity over time. Among the tested strains, Nannochloropsis sp. showed highest fatty acid content (45%w/w) and productivity (238 mg L-1 day-1) aswell as PSII maximum efficiency, demonstrating to be the most suitable strain, of those tested, for lipid production. This study highlights that for microalgae, maintaining a high photosynthetic efficiency during stress is the key to maintain high fatty acid productivities overtime and should be considered when selecting strains for microalgal lipid production.

AB - An economically feasible microalgal lipid industry heavily relies on the selection of suitable strains. Because microalgae lipid content increases under a range of adverse conditions (e.g. nutrient deprivation, high light intensity), photosynthetic activity is usually strongly reduced. As a consequence, lipid productivity rapidly declines overtime, after reaching a maximum within the first days of cultivation. The microalgae Chlorella vulgaris, Chlorococcum littorale, Nannochloropsis oculata, Nannochloropsis sp., Neochloris oleoabundans, Stichococcus bacillaris and Tetraselmis suecica were compared on fatty acid content and productivity, and also on photosynthetic activity under nitrogen (N) starvation. Cultures in N-replete conditions were used as reference. Photosystem II (PSII) maximumefficiency was followed during the experiment, as proxy for the change in photosynthetic activity of the cells. Strains with a high capacity for both lipid accumulation as well as high photosynthetic activity under N starvation exhibited a high lipid productivity over time. Among the tested strains, Nannochloropsis sp. showed highest fatty acid content (45%w/w) and productivity (238 mg L-1 day-1) aswell as PSII maximum efficiency, demonstrating to be the most suitable strain, of those tested, for lipid production. This study highlights that for microalgae, maintaining a high photosynthetic efficiency during stress is the key to maintain high fatty acid productivities overtime and should be considered when selecting strains for microalgal lipid production.

KW - neochloris-oleoabundans

KW - parietochloris-incisa

KW - marine-phytoplankton

KW - biodiesel production

KW - nutrient stress

KW - fatty-acids

KW - light

KW - accumulation

KW - efficiency

KW - biofuels

U2 - 10.1007/s10811-014-0470-8

DO - 10.1007/s10811-014-0470-8

M3 - Article

VL - 27

SP - 1425

EP - 1431

JO - Journal of Applied Phycology

JF - Journal of Applied Phycology

SN - 0921-8971

IS - 4

ER -