Seed transmission of Pepino mosaic virus in tomato

I.M. Hanssen; R. Mumford; D.R. Blystad; I. Cortez; B. Hasiów-AJaroszewska; D. Hristova; I. Pagán; A.M. Pereira; J. Peters; H. Pospieszny; M. Ravnikar; I. Stijger; L. Tomassoli; C. Varveri; R.A.A. van der Vlugt; S.L. Nielsen

doi:10.1007/s10658-009-9528-x

Seed transmission of Pepino mosaic virus in tomato

I.M. Hanssen, R. Mumford, D.R. Blystad, I. Cortez, B. Hasiów-AJaroszewska, D. Hristova, I. Pagán, A.M. Pereira, J. Peters, H. Pospieszny, M. Ravnikar, I. Stijger, L. Tomassoli, C. Varveri, R.A.A. van der Vlugt, S.L. Nielsen

Laboratory of Phytopathology

Research output: Contribution to journal › Article › Academic › peer-review

57 Citations (Scopus)

Abstract

In this manuscript we provide evidence for the seed transmission of Pepino mosaic virus (PepMV) in tomato. Fruit was harvested from a tomato crop artificially infected with both European and CH2 genotypes of PepMV and more than 100,000 seeds were extracted and cleaned using an enzymatic treatment without disinfection. Infection assays using indicator plants confirmed the presence of viable virus on the seeds. Seeds were distributed to ten different laboratories in three separate batches, where they were germinated and the young plants tested by ELISA. In total over 87,000 plants were tested and 23 positives detected, indicating an overall transmission rate of 0.026%. However, the observed seed transmission rates varied from 0.005% to 0.057%, depending on the seed batch used. Results clearly showed that PepMV can be transmitted from seeds contaminated with virus to seedlings, highlighting the risk of using seeds from PepMV-infected plants and the potential for seed transmission to contribute to the further spread of PepMV

Original language	English
Pages (from-to)	145-152
Journal	European Journal of Plant Pathology
Volume	126
Issue number	2
DOIs	https://doi.org/10.1007/s10658-009-9528-x
Publication status	Published - 2010

Keywords

genomic rna
greenhouse tomatoes
sequence
isolate
population
infection
protein
number
crops
uk

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10.1007/s10658-009-9528-x

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Hanssen, I. M., Mumford, R., Blystad, D. R., Cortez, I., Hasiów-AJaroszewska, B., Hristova, D., Pagán, I., Pereira, A. M., Peters, J., Pospieszny, H., Ravnikar, M., Stijger, I., Tomassoli, L., Varveri, C., van der Vlugt, R. A. A., & Nielsen, S. L. (2010). Seed transmission of Pepino mosaic virus in tomato. European Journal of Plant Pathology, 126(2), 145-152. https://doi.org/10.1007/s10658-009-9528-x

@article{8e76bbfa0cd649929ad8b0a839269a79,

title = "Seed transmission of Pepino mosaic virus in tomato",

abstract = "In this manuscript we provide evidence for the seed transmission of Pepino mosaic virus (PepMV) in tomato. Fruit was harvested from a tomato crop artificially infected with both European and CH2 genotypes of PepMV and more than 100,000 seeds were extracted and cleaned using an enzymatic treatment without disinfection. Infection assays using indicator plants confirmed the presence of viable virus on the seeds. Seeds were distributed to ten different laboratories in three separate batches, where they were germinated and the young plants tested by ELISA. In total over 87,000 plants were tested and 23 positives detected, indicating an overall transmission rate of 0.026%. However, the observed seed transmission rates varied from 0.005% to 0.057%, depending on the seed batch used. Results clearly showed that PepMV can be transmitted from seeds contaminated with virus to seedlings, highlighting the risk of using seeds from PepMV-infected plants and the potential for seed transmission to contribute to the further spread of PepMV",

keywords = "genomic rna, greenhouse tomatoes, sequence, isolate, population, infection, protein, number, crops, uk",

author = "I.M. Hanssen and R. Mumford and D.R. Blystad and I. Cortez and B. Hasi{\'o}w-AJaroszewska and D. Hristova and I. Pag{\'a}n and A.M. Pereira and J. Peters and H. Pospieszny and M. Ravnikar and I. Stijger and L. Tomassoli and C. Varveri and {van der Vlugt}, R.A.A. and S.L. Nielsen",

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doi = "10.1007/s10658-009-9528-x",

language = "English",

volume = "126",

pages = "145--152",

journal = "European Journal of Plant Pathology",

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Hanssen, IM, Mumford, R, Blystad, DR, Cortez, I, Hasiów-AJaroszewska, B, Hristova, D, Pagán, I, Pereira, AM, Peters, J, Pospieszny, H, Ravnikar, M, Stijger, I, Tomassoli, L, Varveri, C, van der Vlugt, RAA & Nielsen, SL 2010, 'Seed transmission of Pepino mosaic virus in tomato', European Journal of Plant Pathology, vol. 126, no. 2, pp. 145-152. https://doi.org/10.1007/s10658-009-9528-x

TY - JOUR

T1 - Seed transmission of Pepino mosaic virus in tomato

AU - Hanssen, I.M.

AU - Mumford, R.

AU - Blystad, D.R.

AU - Cortez, I.

AU - Hasiów-AJaroszewska, B.

AU - Hristova, D.

AU - Pagán, I.

AU - Pereira, A.M.

AU - Peters, J.

AU - Pospieszny, H.

AU - Ravnikar, M.

AU - Stijger, I.

AU - Tomassoli, L.

AU - Varveri, C.

AU - van der Vlugt, R.A.A.

AU - Nielsen, S.L.

PY - 2010

Y1 - 2010

N2 - In this manuscript we provide evidence for the seed transmission of Pepino mosaic virus (PepMV) in tomato. Fruit was harvested from a tomato crop artificially infected with both European and CH2 genotypes of PepMV and more than 100,000 seeds were extracted and cleaned using an enzymatic treatment without disinfection. Infection assays using indicator plants confirmed the presence of viable virus on the seeds. Seeds were distributed to ten different laboratories in three separate batches, where they were germinated and the young plants tested by ELISA. In total over 87,000 plants were tested and 23 positives detected, indicating an overall transmission rate of 0.026%. However, the observed seed transmission rates varied from 0.005% to 0.057%, depending on the seed batch used. Results clearly showed that PepMV can be transmitted from seeds contaminated with virus to seedlings, highlighting the risk of using seeds from PepMV-infected plants and the potential for seed transmission to contribute to the further spread of PepMV

AB - In this manuscript we provide evidence for the seed transmission of Pepino mosaic virus (PepMV) in tomato. Fruit was harvested from a tomato crop artificially infected with both European and CH2 genotypes of PepMV and more than 100,000 seeds were extracted and cleaned using an enzymatic treatment without disinfection. Infection assays using indicator plants confirmed the presence of viable virus on the seeds. Seeds were distributed to ten different laboratories in three separate batches, where they were germinated and the young plants tested by ELISA. In total over 87,000 plants were tested and 23 positives detected, indicating an overall transmission rate of 0.026%. However, the observed seed transmission rates varied from 0.005% to 0.057%, depending on the seed batch used. Results clearly showed that PepMV can be transmitted from seeds contaminated with virus to seedlings, highlighting the risk of using seeds from PepMV-infected plants and the potential for seed transmission to contribute to the further spread of PepMV

KW - genomic rna

KW - greenhouse tomatoes

KW - sequence

KW - isolate

KW - population

KW - infection

KW - protein

KW - number

KW - crops

KW - uk

U2 - 10.1007/s10658-009-9528-x

DO - 10.1007/s10658-009-9528-x

M3 - Article

SN - 0929-1873

VL - 126

SP - 145

EP - 152

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

IS - 2

ER -

Seed transmission of Pepino mosaic virus in tomato

Abstract

Keywords

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