Seed maturation and post-harvest ripening negatively affect arabidopsis somatic embryogenesis

Han Wu, Baojian Chen, Martijn Fiers, Justyna Wróbel-Marek, Jan Kodde, Steven P.C. Groot, Gerco Angenent, Hui Feng*, Leónie Bentsink, Kim Boutilier

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Plant development is highly malleable, as evidenced by the ability of cultured cells, tissues and organs to regenerate into whole plants in vitro. The ability of plants to regenerate in vitro is influenced by many different factors, including the donor plant growth conditions and the type of explant. Empirical trial and error manipulation of these and other culture parameters is the basis for improving plant regeneration protocols, but the mechanisms underlying the effects of these parameters on plant regeneration are unknown. Somatic embryogenesis (SE) is a type of in vitro plant regeneration where somatic/vegetative cells are induced to form embryos. Here we show that seed maturation is one of the parameters that affects the ability of germinating embryos to undergo auxin-induced somatic embryogenesis in Arabidopsis thaliana. Late maturation stage seeds harvested from yellow siliques have a higher capacity for somatic embryogenesis than seeds harvested later from brown siliques, a process that can be mimicked by post-harvest storage. Physiological and genetic analyses suggest that an oxidizing environment and ABA metabolism enhance the rate at which germinating embryos lose capacity to reactivate embryogenic growth. Our data suggest that there is a narrow window during late seed maturation in which embryogenic competence is reduced, and that this process also takes place, albeit more slowly, during seed storage. This knowledge provides a framework for identifying new plant totipotency factors and for directing efficient SE in systems that make use of mature seed explants.

Original languageEnglish
Pages (from-to)17-27
JournalPlant Cell, Tissue and Organ Culture
Volume139
Issue number1
Early online date7 Aug 2019
DOIs
Publication statusPublished - Oct 2019

Fingerprint

seed maturation
somatic embryogenesis
ripening
Arabidopsis
embryo (plant)
explants
seeds
totipotency
seed storage
vegetative cells
somatic cells
cultured cells
plant development
auxins
Arabidopsis thaliana
plant growth
metabolism

Keywords

  • ABA
  • Arabidopsis
  • Auxin
  • Post-harvest ripening
  • ROS
  • Seed maturation
  • Somatic embryogenesis

Cite this

@article{e9ca7dee3843478094701e1c2bebf980,
title = "Seed maturation and post-harvest ripening negatively affect arabidopsis somatic embryogenesis",
abstract = "Plant development is highly malleable, as evidenced by the ability of cultured cells, tissues and organs to regenerate into whole plants in vitro. The ability of plants to regenerate in vitro is influenced by many different factors, including the donor plant growth conditions and the type of explant. Empirical trial and error manipulation of these and other culture parameters is the basis for improving plant regeneration protocols, but the mechanisms underlying the effects of these parameters on plant regeneration are unknown. Somatic embryogenesis (SE) is a type of in vitro plant regeneration where somatic/vegetative cells are induced to form embryos. Here we show that seed maturation is one of the parameters that affects the ability of germinating embryos to undergo auxin-induced somatic embryogenesis in Arabidopsis thaliana. Late maturation stage seeds harvested from yellow siliques have a higher capacity for somatic embryogenesis than seeds harvested later from brown siliques, a process that can be mimicked by post-harvest storage. Physiological and genetic analyses suggest that an oxidizing environment and ABA metabolism enhance the rate at which germinating embryos lose capacity to reactivate embryogenic growth. Our data suggest that there is a narrow window during late seed maturation in which embryogenic competence is reduced, and that this process also takes place, albeit more slowly, during seed storage. This knowledge provides a framework for identifying new plant totipotency factors and for directing efficient SE in systems that make use of mature seed explants.",
keywords = "ABA, Arabidopsis, Auxin, Post-harvest ripening, ROS, Seed maturation, Somatic embryogenesis",
author = "Han Wu and Baojian Chen and Martijn Fiers and Justyna Wr{\'o}bel-Marek and Jan Kodde and Groot, {Steven P.C.} and Gerco Angenent and Hui Feng and Le{\'o}nie Bentsink and Kim Boutilier",
year = "2019",
month = "10",
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language = "English",
volume = "139",
pages = "17--27",
journal = "Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants",
issn = "0167-6857",
publisher = "Springer Verlag",
number = "1",

}

Seed maturation and post-harvest ripening negatively affect arabidopsis somatic embryogenesis. / Wu, Han; Chen, Baojian; Fiers, Martijn; Wróbel-Marek, Justyna; Kodde, Jan; Groot, Steven P.C.; Angenent, Gerco; Feng, Hui; Bentsink, Leónie; Boutilier, Kim.

In: Plant Cell, Tissue and Organ Culture, Vol. 139, No. 1, 10.2019, p. 17-27.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Seed maturation and post-harvest ripening negatively affect arabidopsis somatic embryogenesis

AU - Wu, Han

AU - Chen, Baojian

AU - Fiers, Martijn

AU - Wróbel-Marek, Justyna

AU - Kodde, Jan

AU - Groot, Steven P.C.

AU - Angenent, Gerco

AU - Feng, Hui

AU - Bentsink, Leónie

AU - Boutilier, Kim

PY - 2019/10

Y1 - 2019/10

N2 - Plant development is highly malleable, as evidenced by the ability of cultured cells, tissues and organs to regenerate into whole plants in vitro. The ability of plants to regenerate in vitro is influenced by many different factors, including the donor plant growth conditions and the type of explant. Empirical trial and error manipulation of these and other culture parameters is the basis for improving plant regeneration protocols, but the mechanisms underlying the effects of these parameters on plant regeneration are unknown. Somatic embryogenesis (SE) is a type of in vitro plant regeneration where somatic/vegetative cells are induced to form embryos. Here we show that seed maturation is one of the parameters that affects the ability of germinating embryos to undergo auxin-induced somatic embryogenesis in Arabidopsis thaliana. Late maturation stage seeds harvested from yellow siliques have a higher capacity for somatic embryogenesis than seeds harvested later from brown siliques, a process that can be mimicked by post-harvest storage. Physiological and genetic analyses suggest that an oxidizing environment and ABA metabolism enhance the rate at which germinating embryos lose capacity to reactivate embryogenic growth. Our data suggest that there is a narrow window during late seed maturation in which embryogenic competence is reduced, and that this process also takes place, albeit more slowly, during seed storage. This knowledge provides a framework for identifying new plant totipotency factors and for directing efficient SE in systems that make use of mature seed explants.

AB - Plant development is highly malleable, as evidenced by the ability of cultured cells, tissues and organs to regenerate into whole plants in vitro. The ability of plants to regenerate in vitro is influenced by many different factors, including the donor plant growth conditions and the type of explant. Empirical trial and error manipulation of these and other culture parameters is the basis for improving plant regeneration protocols, but the mechanisms underlying the effects of these parameters on plant regeneration are unknown. Somatic embryogenesis (SE) is a type of in vitro plant regeneration where somatic/vegetative cells are induced to form embryos. Here we show that seed maturation is one of the parameters that affects the ability of germinating embryos to undergo auxin-induced somatic embryogenesis in Arabidopsis thaliana. Late maturation stage seeds harvested from yellow siliques have a higher capacity for somatic embryogenesis than seeds harvested later from brown siliques, a process that can be mimicked by post-harvest storage. Physiological and genetic analyses suggest that an oxidizing environment and ABA metabolism enhance the rate at which germinating embryos lose capacity to reactivate embryogenic growth. Our data suggest that there is a narrow window during late seed maturation in which embryogenic competence is reduced, and that this process also takes place, albeit more slowly, during seed storage. This knowledge provides a framework for identifying new plant totipotency factors and for directing efficient SE in systems that make use of mature seed explants.

KW - ABA

KW - Arabidopsis

KW - Auxin

KW - Post-harvest ripening

KW - ROS

KW - Seed maturation

KW - Somatic embryogenesis

U2 - 10.1007/s11240-019-01658-8

DO - 10.1007/s11240-019-01658-8

M3 - Article

VL - 139

SP - 17

EP - 27

JO - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants

JF - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants

SN - 0167-6857

IS - 1

ER -