Secreted antigens of Mycobacterium avium subspecies paratuberculosis as prominent immune targets

P.T.J. Willemsen, S.F. Harders-Westerveen, A. Dinkla, D. Bakker, F.G. van Zijderveld, J.E.R. Thole

    Research output: Contribution to journalArticleAcademicpeer-review

    30 Citations (Scopus)

    Abstract

    We here describe the identification and characterization of three novel secreted Mycobacterium avium subsp. paratuberculosis antigens of 9, 15 and 34 kDa (Map2609, Map2942c and Map0210c, respectively) by screening a genomic expression library with a serum of a naturally infected clinical cow. The 9, 15 and 34 kDa antigens display strong homology to previously described M. tuberculosis antigens, TB8.4, MPT53 and Erp, respectively. Furthermore, these antigens were shown to be recognized by antibodies from infected cattle, when tested with a limited number of sera from subclinical (n = 7) and clinical (n = 3) infected cattle.
    Original languageEnglish
    Pages (from-to)337-344
    JournalVeterinary Microbiology
    Volume114
    Issue number3-4
    DOIs
    Publication statusPublished - 2006

    Keywords

    • heat-shock protein
    • nucleotide-sequence
    • molecular-cloning
    • johnes-disease
    • tuberculosis
    • gene
    • expression
    • reactivity
    • identification
    • antibodies

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