Secreted antigens of Mycobacterium avium subspecies paratuberculosis as prominent immune targets

P.T.J. Willemsen; S.F. Harders-Westerveen; A. Dinkla; D. Bakker; F.G. van Zijderveld; J.E.R. Thole

doi:10.1016/j.vetmic.2005.12.005

Secreted antigens of Mycobacterium avium subspecies paratuberculosis as prominent immune targets

P.T.J. Willemsen, S.F. Harders-Westerveen, A. Dinkla, D. Bakker, F.G. van Zijderveld, J.E.R. Thole

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

We here describe the identification and characterization of three novel secreted Mycobacterium avium subsp. paratuberculosis antigens of 9, 15 and 34 kDa (Map2609, Map2942c and Map0210c, respectively) by screening a genomic expression library with a serum of a naturally infected clinical cow. The 9, 15 and 34 kDa antigens display strong homology to previously described M. tuberculosis antigens, TB8.4, MPT53 and Erp, respectively. Furthermore, these antigens were shown to be recognized by antibodies from infected cattle, when tested with a limited number of sera from subclinical (n = 7) and clinical (n = 3) infected cattle.

Original language	English
Pages (from-to)	337-344
Journal	Veterinary Microbiology
Volume	114
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetmic.2005.12.005
Publication status	Published - 2006

Keywords

heat-shock protein
nucleotide-sequence
molecular-cloning
johnes-disease
tuberculosis
gene
expression
reactivity
identification
antibodies

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vetmic.2005.12.005

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title = "Secreted antigens of Mycobacterium avium subspecies paratuberculosis as prominent immune targets",

abstract = "We here describe the identification and characterization of three novel secreted Mycobacterium avium subsp. paratuberculosis antigens of 9, 15 and 34 kDa (Map2609, Map2942c and Map0210c, respectively) by screening a genomic expression library with a serum of a naturally infected clinical cow. The 9, 15 and 34 kDa antigens display strong homology to previously described M. tuberculosis antigens, TB8.4, MPT53 and Erp, respectively. Furthermore, these antigens were shown to be recognized by antibodies from infected cattle, when tested with a limited number of sera from subclinical (n = 7) and clinical (n = 3) infected cattle.",

keywords = "heat-shock protein, nucleotide-sequence, molecular-cloning, johnes-disease, tuberculosis, gene, expression, reactivity, identification, antibodies",

author = "P.T.J. Willemsen and S.F. Harders-Westerveen and A. Dinkla and D. Bakker and {van Zijderveld}, F.G. and J.E.R. Thole",

year = "2006",

doi = "10.1016/j.vetmic.2005.12.005",

language = "English",

volume = "114",

pages = "337--344",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier",

number = "3-4",

}

TY - JOUR

T1 - Secreted antigens of Mycobacterium avium subspecies paratuberculosis as prominent immune targets

AU - Willemsen, P.T.J.

AU - Harders-Westerveen, S.F.

AU - Dinkla, A.

AU - Bakker, D.

AU - van Zijderveld, F.G.

AU - Thole, J.E.R.

PY - 2006

Y1 - 2006

N2 - We here describe the identification and characterization of three novel secreted Mycobacterium avium subsp. paratuberculosis antigens of 9, 15 and 34 kDa (Map2609, Map2942c and Map0210c, respectively) by screening a genomic expression library with a serum of a naturally infected clinical cow. The 9, 15 and 34 kDa antigens display strong homology to previously described M. tuberculosis antigens, TB8.4, MPT53 and Erp, respectively. Furthermore, these antigens were shown to be recognized by antibodies from infected cattle, when tested with a limited number of sera from subclinical (n = 7) and clinical (n = 3) infected cattle.

AB - We here describe the identification and characterization of three novel secreted Mycobacterium avium subsp. paratuberculosis antigens of 9, 15 and 34 kDa (Map2609, Map2942c and Map0210c, respectively) by screening a genomic expression library with a serum of a naturally infected clinical cow. The 9, 15 and 34 kDa antigens display strong homology to previously described M. tuberculosis antigens, TB8.4, MPT53 and Erp, respectively. Furthermore, these antigens were shown to be recognized by antibodies from infected cattle, when tested with a limited number of sera from subclinical (n = 7) and clinical (n = 3) infected cattle.

KW - heat-shock protein

KW - nucleotide-sequence

KW - molecular-cloning

KW - johnes-disease

KW - tuberculosis

KW - gene

KW - expression

KW - reactivity

KW - identification

KW - antibodies

U2 - 10.1016/j.vetmic.2005.12.005

DO - 10.1016/j.vetmic.2005.12.005

M3 - Article

SN - 0378-1135

VL - 114

SP - 337

EP - 344

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 3-4

ER -

Secreted antigens of Mycobacterium avium subspecies paratuberculosis as prominent immune targets

Abstract

Keywords

UN SDGs

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