Screening of protein biomarkers for sports doping and veterinary control

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

There are similarities between sports doping and veterinary control. Prohibited substances (e.g., anabolic agents and peptide hormones) are similar, and immunoassays and chromatography-mass spectrometry are applied as analytical methods in both worlds. In recent years, detection strategies based on protein biomarkers were successfully developed and adopted in sports control. When measuring biomarkers, the window of detection can be extended due to a prolonged biological response, so a whole range of substances may be tackled in an indirect manner. In view of the similarities in intended biological effects, such as increased muscle mass, we envisage that biomarker-based detection may be adopted veterinary control in future. In this review, we discuss detection strategies based on protein biomarkers for biomarker discovery and method development. With the lessons learnt from successfully implementing biomarker strategies in doping regulations, we advocate adoption in the veterinary world and revision of the current restrictive regulations concerning analytical methods.
Original languageEnglish
Pages (from-to)47-63
JournalTrAC : Trends in Analytical Chemistry
Volume57
DOIs
Publication statusPublished - 2014

Fingerprint

Biomarkers
Sports
Screening
Doping (additives)
Proteins
Anabolic Agents
Peptide Hormones
Chromatography
Mass spectrometry
Muscle

Keywords

  • recombinant bovine somatotropin
  • growth-hormone treatment
  • igf-i
  • mass-spectrometry
  • skeletal-muscle
  • promoter abuse
  • insulin misuse
  • veal calves
  • serum
  • gh

Cite this

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title = "Screening of protein biomarkers for sports doping and veterinary control",
abstract = "There are similarities between sports doping and veterinary control. Prohibited substances (e.g., anabolic agents and peptide hormones) are similar, and immunoassays and chromatography-mass spectrometry are applied as analytical methods in both worlds. In recent years, detection strategies based on protein biomarkers were successfully developed and adopted in sports control. When measuring biomarkers, the window of detection can be extended due to a prolonged biological response, so a whole range of substances may be tackled in an indirect manner. In view of the similarities in intended biological effects, such as increased muscle mass, we envisage that biomarker-based detection may be adopted veterinary control in future. In this review, we discuss detection strategies based on protein biomarkers for biomarker discovery and method development. With the lessons learnt from successfully implementing biomarker strategies in doping regulations, we advocate adoption in the veterinary world and revision of the current restrictive regulations concerning analytical methods.",
keywords = "recombinant bovine somatotropin, growth-hormone treatment, igf-i, mass-spectrometry, skeletal-muscle, promoter abuse, insulin misuse, veal calves, serum, gh",
author = "S.K.J. Ludwig and {van Ginkel}, L.A. and M.W.F. Nielen",
year = "2014",
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language = "English",
volume = "57",
pages = "47--63",
journal = "TrAC : Trends in Analytical Chemistry",
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Screening of protein biomarkers for sports doping and veterinary control. / Ludwig, S.K.J.; van Ginkel, L.A.; Nielen, M.W.F.

In: TrAC : Trends in Analytical Chemistry, Vol. 57, 2014, p. 47-63.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Screening of protein biomarkers for sports doping and veterinary control

AU - Ludwig, S.K.J.

AU - van Ginkel, L.A.

AU - Nielen, M.W.F.

PY - 2014

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AB - There are similarities between sports doping and veterinary control. Prohibited substances (e.g., anabolic agents and peptide hormones) are similar, and immunoassays and chromatography-mass spectrometry are applied as analytical methods in both worlds. In recent years, detection strategies based on protein biomarkers were successfully developed and adopted in sports control. When measuring biomarkers, the window of detection can be extended due to a prolonged biological response, so a whole range of substances may be tackled in an indirect manner. In view of the similarities in intended biological effects, such as increased muscle mass, we envisage that biomarker-based detection may be adopted veterinary control in future. In this review, we discuss detection strategies based on protein biomarkers for biomarker discovery and method development. With the lessons learnt from successfully implementing biomarker strategies in doping regulations, we advocate adoption in the veterinary world and revision of the current restrictive regulations concerning analytical methods.

KW - recombinant bovine somatotropin

KW - growth-hormone treatment

KW - igf-i

KW - mass-spectrometry

KW - skeletal-muscle

KW - promoter abuse

KW - insulin misuse

KW - veal calves

KW - serum

KW - gh

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JO - TrAC : Trends in Analytical Chemistry

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