Screening of bacterial isolates from various European soils for in vitro antagonistic activity towards Rhizoctonia solani and Fusarium oxysporum: Site-dependent composition and diversity revealed

M.F. Adesina, A. Lembke, R. Costa, A.G.C.L. Speksnijder, K. Smalla

    Research output: Contribution to journalArticleAcademicpeer-review

    72 Citations (Scopus)

    Abstract

    A cultivation-based approach was used to determine the in vitro antagonistic potential of soil bacteria towards Rhizoctonia solani AG3 and Fusarium oxysporum f. sp. lini (Foln3). Four composite soil samples were collected from four agricultural sites with previous documentation of disease suppression, located in France (FR), the Netherlands (NL), Sweden (SE) and the United Kingdom (UK). Similarly, two sites from Germany (Berlin, G-BR; and Braunschweig, G-BS) without documentation of disease suppression were sampled. Total bacterial counts were determined by plating serial dilutions from the composite soil samples onto R2A, AGS and King's B media. A total of 1,788 isolates (approximately 100 isolates per medium and site) was screened for antifungal activity, and in vitro antagonists (327 isolates) were found amongst the dominant culturable bacteria isolated from all six soils. The overall proportion of antagonists and the number of isolates with inhibitory activity against F. oxysporum were highest in three of the suppressive soils (FR, NL and SE). Characterization of antagonistic bacteria revealed a high phenotypic and genotypic diversity. Siderophore and protease activity were the most prominent phenotypic traits amongst the antagonists. The composition and diversity of antagonists in each soil was site-specific. Nevertheless, none of the antimicrobial traits of bacteria potentially contributing to soil suppressiveness analyzed in this study could be regarded as specific to a given site.
    Original languageEnglish
    Pages (from-to)2818-2828
    JournalSoil Biology and Biochemistry
    Volume39
    Issue number11
    DOIs
    Publication statusPublished - 2007

    Keywords

    • microbial community structure
    • biological-control
    • suppressive soils
    • plant-pathogens
    • pseudomonas
    • wilt
    • microorganisms
    • rhizosphere
    • mechanisms
    • diseases

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