Screening of apple cultivars for resistance to European canker, Neonectria ditissima

L. Garkava-Gustavsson, A. Zborowska, J. Sehic, M. Rur, H. Nybom, J.E. Englund, M. Lateur, W.E. van de Weg, A. Holefors

Research output: Chapter in Book/Report/Conference proceedingConference paper

15 Citations (Scopus)

Abstract

European canker, caused by the fungus Neonectria ditissima, is a severe problem in apple production both in Sweden and in many other northern European countries. Even when applying fungicides and good horticultural practices, canker damage occurs almost yearly in nurseries and orchards. Some years, devastating outbreaks destroy numerous trees. To date, complete resistance to N. ditissima is not known in apple. For further research and plant breeding, heritable variation in quantitative resistance should be investigated by phenotyping large sets of cultivars. In the present project, 55 apple cultivars were screened for resistance to N. ditissima. One-year-old shoots from mature trees were inoculated in the greenhouse with a standardized volume and concentration of conidia suspension using different inoculation methods. Two-year-old trees of five cultivars were inoculated in the field. Length of the occurring cankers was measured at regular intervals throughout a period of up to three months. The investigated cultivars showed considerable differences in colonization rate. In cultivars known to be highly resistant, i.e., ‘Santana’, lesions progressed much slower compared to susceptible cultivars like ‘Cox’s Orange Pippin’ and ‘James Grieve’. Since the inoculation-based phenotyping is demanding in labour and time (duration), especially when the test is performed on grafted trees, qPCR-based assessment of fungal biomass at early stages of infection was explored as an alternative or complementary approach for phenotyping.
Original languageEnglish
Title of host publicationXIII Eucarpia Symposium on Fruit Breeding and Genetics, Warsaw, Poland
EditorsK.M. Evans, B. Lata, M. Kellerhals
PublisherISHS
Pages529-536
Volume976
ISBN (Print)9789066051195
DOIs
Publication statusPublished - 2013
EventXIII Eucarpia Symposium on Fruit Breeding and Genetics -
Duration: 11 Sep 201115 Sep 2011

Conference

ConferenceXIII Eucarpia Symposium on Fruit Breeding and Genetics
Period11/09/1115/09/11

Fingerprint

Neonectria ditissima
apples
screening
cultivars
phenotype
cankers (plants)
inoculation methods
lesions (plant)
Northern European region
plant breeding
conidia
Sweden
fungicides
labor
orchards
greenhouses
fungi
shoots
duration
biomass

Cite this

Garkava-Gustavsson, L., Zborowska, A., Sehic, J., Rur, M., Nybom, H., Englund, J. E., ... Holefors, A. (2013). Screening of apple cultivars for resistance to European canker, Neonectria ditissima. In K. M. Evans, B. Lata, & M. Kellerhals (Eds.), XIII Eucarpia Symposium on Fruit Breeding and Genetics, Warsaw, Poland (Vol. 976, pp. 529-536). ISHS. https://doi.org/10.17660/ActaHortic.2013.976.75
Garkava-Gustavsson, L. ; Zborowska, A. ; Sehic, J. ; Rur, M. ; Nybom, H. ; Englund, J.E. ; Lateur, M. ; van de Weg, W.E. ; Holefors, A. / Screening of apple cultivars for resistance to European canker, Neonectria ditissima. XIII Eucarpia Symposium on Fruit Breeding and Genetics, Warsaw, Poland. editor / K.M. Evans ; B. Lata ; M. Kellerhals. Vol. 976 ISHS, 2013. pp. 529-536
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title = "Screening of apple cultivars for resistance to European canker, Neonectria ditissima",
abstract = "European canker, caused by the fungus Neonectria ditissima, is a severe problem in apple production both in Sweden and in many other northern European countries. Even when applying fungicides and good horticultural practices, canker damage occurs almost yearly in nurseries and orchards. Some years, devastating outbreaks destroy numerous trees. To date, complete resistance to N. ditissima is not known in apple. For further research and plant breeding, heritable variation in quantitative resistance should be investigated by phenotyping large sets of cultivars. In the present project, 55 apple cultivars were screened for resistance to N. ditissima. One-year-old shoots from mature trees were inoculated in the greenhouse with a standardized volume and concentration of conidia suspension using different inoculation methods. Two-year-old trees of five cultivars were inoculated in the field. Length of the occurring cankers was measured at regular intervals throughout a period of up to three months. The investigated cultivars showed considerable differences in colonization rate. In cultivars known to be highly resistant, i.e., ‘Santana’, lesions progressed much slower compared to susceptible cultivars like ‘Cox’s Orange Pippin’ and ‘James Grieve’. Since the inoculation-based phenotyping is demanding in labour and time (duration), especially when the test is performed on grafted trees, qPCR-based assessment of fungal biomass at early stages of infection was explored as an alternative or complementary approach for phenotyping.",
author = "L. Garkava-Gustavsson and A. Zborowska and J. Sehic and M. Rur and H. Nybom and J.E. Englund and M. Lateur and {van de Weg}, W.E. and A. Holefors",
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Garkava-Gustavsson, L, Zborowska, A, Sehic, J, Rur, M, Nybom, H, Englund, JE, Lateur, M, van de Weg, WE & Holefors, A 2013, Screening of apple cultivars for resistance to European canker, Neonectria ditissima. in KM Evans, B Lata & M Kellerhals (eds), XIII Eucarpia Symposium on Fruit Breeding and Genetics, Warsaw, Poland. vol. 976, ISHS, pp. 529-536, XIII Eucarpia Symposium on Fruit Breeding and Genetics, 11/09/11. https://doi.org/10.17660/ActaHortic.2013.976.75

Screening of apple cultivars for resistance to European canker, Neonectria ditissima. / Garkava-Gustavsson, L.; Zborowska, A.; Sehic, J.; Rur, M.; Nybom, H.; Englund, J.E.; Lateur, M.; van de Weg, W.E.; Holefors, A.

XIII Eucarpia Symposium on Fruit Breeding and Genetics, Warsaw, Poland. ed. / K.M. Evans; B. Lata; M. Kellerhals. Vol. 976 ISHS, 2013. p. 529-536.

Research output: Chapter in Book/Report/Conference proceedingConference paper

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AU - Garkava-Gustavsson, L.

AU - Zborowska, A.

AU - Sehic, J.

AU - Rur, M.

AU - Nybom, H.

AU - Englund, J.E.

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AU - van de Weg, W.E.

AU - Holefors, A.

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N2 - European canker, caused by the fungus Neonectria ditissima, is a severe problem in apple production both in Sweden and in many other northern European countries. Even when applying fungicides and good horticultural practices, canker damage occurs almost yearly in nurseries and orchards. Some years, devastating outbreaks destroy numerous trees. To date, complete resistance to N. ditissima is not known in apple. For further research and plant breeding, heritable variation in quantitative resistance should be investigated by phenotyping large sets of cultivars. In the present project, 55 apple cultivars were screened for resistance to N. ditissima. One-year-old shoots from mature trees were inoculated in the greenhouse with a standardized volume and concentration of conidia suspension using different inoculation methods. Two-year-old trees of five cultivars were inoculated in the field. Length of the occurring cankers was measured at regular intervals throughout a period of up to three months. The investigated cultivars showed considerable differences in colonization rate. In cultivars known to be highly resistant, i.e., ‘Santana’, lesions progressed much slower compared to susceptible cultivars like ‘Cox’s Orange Pippin’ and ‘James Grieve’. Since the inoculation-based phenotyping is demanding in labour and time (duration), especially when the test is performed on grafted trees, qPCR-based assessment of fungal biomass at early stages of infection was explored as an alternative or complementary approach for phenotyping.

AB - European canker, caused by the fungus Neonectria ditissima, is a severe problem in apple production both in Sweden and in many other northern European countries. Even when applying fungicides and good horticultural practices, canker damage occurs almost yearly in nurseries and orchards. Some years, devastating outbreaks destroy numerous trees. To date, complete resistance to N. ditissima is not known in apple. For further research and plant breeding, heritable variation in quantitative resistance should be investigated by phenotyping large sets of cultivars. In the present project, 55 apple cultivars were screened for resistance to N. ditissima. One-year-old shoots from mature trees were inoculated in the greenhouse with a standardized volume and concentration of conidia suspension using different inoculation methods. Two-year-old trees of five cultivars were inoculated in the field. Length of the occurring cankers was measured at regular intervals throughout a period of up to three months. The investigated cultivars showed considerable differences in colonization rate. In cultivars known to be highly resistant, i.e., ‘Santana’, lesions progressed much slower compared to susceptible cultivars like ‘Cox’s Orange Pippin’ and ‘James Grieve’. Since the inoculation-based phenotyping is demanding in labour and time (duration), especially when the test is performed on grafted trees, qPCR-based assessment of fungal biomass at early stages of infection was explored as an alternative or complementary approach for phenotyping.

U2 - 10.17660/ActaHortic.2013.976.75

DO - 10.17660/ActaHortic.2013.976.75

M3 - Conference paper

SN - 9789066051195

VL - 976

SP - 529

EP - 536

BT - XIII Eucarpia Symposium on Fruit Breeding and Genetics, Warsaw, Poland

A2 - Evans, K.M.

A2 - Lata, B.

A2 - Kellerhals, M.

PB - ISHS

ER -

Garkava-Gustavsson L, Zborowska A, Sehic J, Rur M, Nybom H, Englund JE et al. Screening of apple cultivars for resistance to European canker, Neonectria ditissima. In Evans KM, Lata B, Kellerhals M, editors, XIII Eucarpia Symposium on Fruit Breeding and Genetics, Warsaw, Poland. Vol. 976. ISHS. 2013. p. 529-536 https://doi.org/10.17660/ActaHortic.2013.976.75