Screening for resistance to the beet armyworm (Spodoptera exigua) in Allium cepa and its wild relatives

S.J. Zheng, B. Henken, E. Sofiari, W.A. Wietsma, E. Jacobsen, F.A. Krens, C. Kik

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

The beet armyworm (Spodoptera exigua Hübner) is the most important pest in tropical Allium cultivations. All shallot ( Allium cepa L. group Aggregatum) cultivars are susceptible to this pest. Therefore accessions from three wild Allium species, namely A. galanthum Kar. et Kir., A. fistulosum L. and A. roylei Stearn, next to A. cepa L. were used to screen for resistance. First of all, a reliable bio-assay had to be developed. To this end transparent plastic cages with in total 5 plants of one accession per cage were placed on perlite in a heated greenhouse. Five 3-day old larvae were inoculated on each plant. Eight days after inoculation the number of surviving larvae per cage and the mean fresh weight per larva was determined. The lowest larval survival (36€was found on A. roylei. This was not, however, significantly different from other Allium accessions. Significant differences were found in the fresh weight per larva fed on different Allium accessions. The larvae survived on A. roylei had a very low fresh weight (10.3 mg per larva), while those on an accession of A. fistulosum had the highest fresh weight (45.1 mg per larva). The larval fresh weight on A. roylei was lower than all the other accessions except from the tropical shallot cultivar Bawang Bali. To check whether or not a toxic compound was involved in the resistance present in A. roylei, ten accessions from four Allium species were screened. Five 3-day old larvae were inoculated on regularly replaced leaf material of each accession of Allium species. No significant differences were found in mean fresh weight per larva and mean survival of larvae among different accessions. There were also no significant differences in pupal weight and developmental time. All larvae became pupae 10 days after inoculation. The data indicate that there is no toxic compound present in A. roylei. These results are underlined by the observation in the greenhouse bio-assay that A. roylei plants were equally damaged by the beet armyworm compared to other Allium species. The results obtained so far therefore suggest that introduction of resistance to S. exigua via the exploitation of variation for resistance to the beet armyworm in A. roylei is unclear and that genetic engineering using Cry sequences could provide a way forward.
Original languageEnglish
Pages (from-to)77-85
JournalEuphytica
Volume114
DOIs
Publication statusPublished - 2000

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Spodoptera
Beta vulgaris
Spodoptera exigua
Onions
wild relatives
Allium cepa
Allium
Larva
screening
larvae
Weights and Measures
Shallots
shallots
cages
Poisons
toxic substances
pests
Allium fistulosum
greenhouses
Pupa

Cite this

@article{57a0878b267c4ff7a8ff8e1a1a4d6ee8,
title = "Screening for resistance to the beet armyworm (Spodoptera exigua) in Allium cepa and its wild relatives",
abstract = "The beet armyworm (Spodoptera exigua H{\"u}bner) is the most important pest in tropical Allium cultivations. All shallot ( Allium cepa L. group Aggregatum) cultivars are susceptible to this pest. Therefore accessions from three wild Allium species, namely A. galanthum Kar. et Kir., A. fistulosum L. and A. roylei Stearn, next to A. cepa L. were used to screen for resistance. First of all, a reliable bio-assay had to be developed. To this end transparent plastic cages with in total 5 plants of one accession per cage were placed on perlite in a heated greenhouse. Five 3-day old larvae were inoculated on each plant. Eight days after inoculation the number of surviving larvae per cage and the mean fresh weight per larva was determined. The lowest larval survival (36€was found on A. roylei. This was not, however, significantly different from other Allium accessions. Significant differences were found in the fresh weight per larva fed on different Allium accessions. The larvae survived on A. roylei had a very low fresh weight (10.3 mg per larva), while those on an accession of A. fistulosum had the highest fresh weight (45.1 mg per larva). The larval fresh weight on A. roylei was lower than all the other accessions except from the tropical shallot cultivar Bawang Bali. To check whether or not a toxic compound was involved in the resistance present in A. roylei, ten accessions from four Allium species were screened. Five 3-day old larvae were inoculated on regularly replaced leaf material of each accession of Allium species. No significant differences were found in mean fresh weight per larva and mean survival of larvae among different accessions. There were also no significant differences in pupal weight and developmental time. All larvae became pupae 10 days after inoculation. The data indicate that there is no toxic compound present in A. roylei. These results are underlined by the observation in the greenhouse bio-assay that A. roylei plants were equally damaged by the beet armyworm compared to other Allium species. The results obtained so far therefore suggest that introduction of resistance to S. exigua via the exploitation of variation for resistance to the beet armyworm in A. roylei is unclear and that genetic engineering using Cry sequences could provide a way forward.",
author = "S.J. Zheng and B. Henken and E. Sofiari and W.A. Wietsma and E. Jacobsen and F.A. Krens and C. Kik",
year = "2000",
doi = "10.1023/A:1004089424419",
language = "English",
volume = "114",
pages = "77--85",
journal = "Euphytica",
issn = "0014-2336",
publisher = "Springer Verlag",

}

Screening for resistance to the beet armyworm (Spodoptera exigua) in Allium cepa and its wild relatives. / Zheng, S.J.; Henken, B.; Sofiari, E.; Wietsma, W.A.; Jacobsen, E.; Krens, F.A.; Kik, C.

In: Euphytica, Vol. 114, 2000, p. 77-85.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Screening for resistance to the beet armyworm (Spodoptera exigua) in Allium cepa and its wild relatives

AU - Zheng, S.J.

AU - Henken, B.

AU - Sofiari, E.

AU - Wietsma, W.A.

AU - Jacobsen, E.

AU - Krens, F.A.

AU - Kik, C.

PY - 2000

Y1 - 2000

N2 - The beet armyworm (Spodoptera exigua Hübner) is the most important pest in tropical Allium cultivations. All shallot ( Allium cepa L. group Aggregatum) cultivars are susceptible to this pest. Therefore accessions from three wild Allium species, namely A. galanthum Kar. et Kir., A. fistulosum L. and A. roylei Stearn, next to A. cepa L. were used to screen for resistance. First of all, a reliable bio-assay had to be developed. To this end transparent plastic cages with in total 5 plants of one accession per cage were placed on perlite in a heated greenhouse. Five 3-day old larvae were inoculated on each plant. Eight days after inoculation the number of surviving larvae per cage and the mean fresh weight per larva was determined. The lowest larval survival (36€was found on A. roylei. This was not, however, significantly different from other Allium accessions. Significant differences were found in the fresh weight per larva fed on different Allium accessions. The larvae survived on A. roylei had a very low fresh weight (10.3 mg per larva), while those on an accession of A. fistulosum had the highest fresh weight (45.1 mg per larva). The larval fresh weight on A. roylei was lower than all the other accessions except from the tropical shallot cultivar Bawang Bali. To check whether or not a toxic compound was involved in the resistance present in A. roylei, ten accessions from four Allium species were screened. Five 3-day old larvae were inoculated on regularly replaced leaf material of each accession of Allium species. No significant differences were found in mean fresh weight per larva and mean survival of larvae among different accessions. There were also no significant differences in pupal weight and developmental time. All larvae became pupae 10 days after inoculation. The data indicate that there is no toxic compound present in A. roylei. These results are underlined by the observation in the greenhouse bio-assay that A. roylei plants were equally damaged by the beet armyworm compared to other Allium species. The results obtained so far therefore suggest that introduction of resistance to S. exigua via the exploitation of variation for resistance to the beet armyworm in A. roylei is unclear and that genetic engineering using Cry sequences could provide a way forward.

AB - The beet armyworm (Spodoptera exigua Hübner) is the most important pest in tropical Allium cultivations. All shallot ( Allium cepa L. group Aggregatum) cultivars are susceptible to this pest. Therefore accessions from three wild Allium species, namely A. galanthum Kar. et Kir., A. fistulosum L. and A. roylei Stearn, next to A. cepa L. were used to screen for resistance. First of all, a reliable bio-assay had to be developed. To this end transparent plastic cages with in total 5 plants of one accession per cage were placed on perlite in a heated greenhouse. Five 3-day old larvae were inoculated on each plant. Eight days after inoculation the number of surviving larvae per cage and the mean fresh weight per larva was determined. The lowest larval survival (36€was found on A. roylei. This was not, however, significantly different from other Allium accessions. Significant differences were found in the fresh weight per larva fed on different Allium accessions. The larvae survived on A. roylei had a very low fresh weight (10.3 mg per larva), while those on an accession of A. fistulosum had the highest fresh weight (45.1 mg per larva). The larval fresh weight on A. roylei was lower than all the other accessions except from the tropical shallot cultivar Bawang Bali. To check whether or not a toxic compound was involved in the resistance present in A. roylei, ten accessions from four Allium species were screened. Five 3-day old larvae were inoculated on regularly replaced leaf material of each accession of Allium species. No significant differences were found in mean fresh weight per larva and mean survival of larvae among different accessions. There were also no significant differences in pupal weight and developmental time. All larvae became pupae 10 days after inoculation. The data indicate that there is no toxic compound present in A. roylei. These results are underlined by the observation in the greenhouse bio-assay that A. roylei plants were equally damaged by the beet armyworm compared to other Allium species. The results obtained so far therefore suggest that introduction of resistance to S. exigua via the exploitation of variation for resistance to the beet armyworm in A. roylei is unclear and that genetic engineering using Cry sequences could provide a way forward.

U2 - 10.1023/A:1004089424419

DO - 10.1023/A:1004089424419

M3 - Article

VL - 114

SP - 77

EP - 85

JO - Euphytica

JF - Euphytica

SN - 0014-2336

ER -