Screening and confirmation criteria for hormone residue analysis using liquid chromatography accurate mass time-of-flight, fourier transform ion cyclotron resonance and orbitrap mass spectrometry techniques

M.W.F. Nielen, M.C. Engelen, R. Zuiderent, R. Ramaker

Research output: Contribution to journalArticleAcademicpeer-review

148 Citations (Scopus)

Abstract

An emerging trend is recognised in hormone and veterinary drug residue analysis from liquid chromatography tandem mass spectrometry (LC/MS/MS) based screening and confirmation towards accurate mass alternatives such as LC coupled with time-of-flight (TOF), Fourier transform ion cyclotron resonance (FTICR) or Fourier transform orbitrap (FT Orbitrap) MS. In this study, mass resolution and accuracy are discussed for LC/MS screening and confirmation of targeted analytes and for the identification of unknowns using the anabolic steroid stanozolol and the designer ß-agonist ¿Clenbuterol-R¿ as model substances. It is shown theoretically and experimentally that mass accuracy criteria without proper mass resolution criteria yield false compliant (false negative) results, both in MS screening and MS/MS confirmation of stanozolol. On the other hand, previous medium resolution accurate mass TOFMS/MS data of the designer ß-agonist were fully confirmed by high resolution FT Orbitrap MSn experiments. A discussion is initiated through a proposal for additional criteria for the use of accurate mass LC/MS technologies, to be implemented in Commission Decision 2002/657/EC
Original languageEnglish
Pages (from-to)122-129
JournalAnalytica Chimica Acta
Volume586
Issue number1-2
DOIs
Publication statusPublished - 2007

Keywords

  • electrospray-ionization
  • urine
  • identification
  • stanozolol
  • steroids

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