Scrapie incidence and PRNP polymorphisms

Rare small ruminant breeds of Sicily with TSE protecting genetic reservoirs

Maria Vitale*, Sergio Migliore, Maria La Giglia, Placido Alberti, Vincenzo Di Marco Lo Presti, Jan P.M. Langeveld

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Background: Transmissible spongiform encephalopathies (TSE) are fatal neurodegenerative diseases of several mammalian species, including humans. In Italy, the active surveillance through rapid tests on brain stem from small ruminants started in 2002 on randomly selected samples of healthy slaughtered animals. Sampling number was proportionally related to the regional small ruminant population. Of the twenty Italian regions, Sicily has the second largest population of small ruminants which is mainly constituted by crossbreed animals (>70 %). Sicily contains also three native sheep breeds Pinzirita, Comisana and Valle del Belice. Native goat breeds are Girgentana, Messinese, Argentata dell'Etna, Maltese and Rossa Mediterranea. The polymorphisms of prion protein gene (PRNP) may influence disease susceptibility and breeding programs for genetic TSE resistance are being applied in sheep. Protective alleles have been recently reported for goats also. These differ from those in sheep and may allow breeding programs in the near future. In this paper the data of active surveillance for scrapie control in general population of small ruminants in Sicily are reported together with the analysis on the polymorphism of PRNP in a number of Sicilian autochthonous breeds. The evaluation of the frequency of protective alleles is fundamental for the implementation of a TSE resistance breeding program. Results: TSE surveillance in small ruminants in Sicily showed a of total fifty seven scrapie outbreaks from 1997 to 2014 involving mainly crossbreed animals. The PRNP polymorphism analysis in autochthonous breeds showed protective allele frequencies of 30-40 % ARR in sheep and 12-18 % K222 in three of the four goat breeds; these breeds are distributed over limited areas of the island. Conclusion: The study on PRNP polymorphisms in Sicilian small ruminant population showed higher frequency of the protective alleles compared to most other European breeds. Our results suggest that PRNP genetic variety in Sicilian sheep and goats can be a resource for TSE resistance breeding programmes while maintaining the conservation of endangered breeds and valorisation of their typical food products.

Original languageEnglish
Article number141
JournalBMC Veterinary Research
Volume12
Issue number1
DOIs
Publication statusPublished - 2016

Fingerprint

Sicily
Scrapie
Prion Diseases
prion diseases
scrapie
prions
Ruminants
small ruminants
genetic polymorphism
Sheep
breeds
Goats
incidence
Breeding
Incidence
Gene Frequency
Genes
sheep
goat breeds
genes

Keywords

  • Autochthonous breeds
  • Breeding programs
  • PRNP polymorphism
  • Scrapie surveillance
  • TSE

Cite this

Vitale, M., Migliore, S., La Giglia, M., Alberti, P., Di Marco Lo Presti, V., & Langeveld, J. P. M. (2016). Scrapie incidence and PRNP polymorphisms: Rare small ruminant breeds of Sicily with TSE protecting genetic reservoirs. BMC Veterinary Research, 12(1), [141]. https://doi.org/10.1186/s12917-016-0766-9
Vitale, Maria ; Migliore, Sergio ; La Giglia, Maria ; Alberti, Placido ; Di Marco Lo Presti, Vincenzo ; Langeveld, Jan P.M. / Scrapie incidence and PRNP polymorphisms : Rare small ruminant breeds of Sicily with TSE protecting genetic reservoirs. In: BMC Veterinary Research. 2016 ; Vol. 12, No. 1.
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title = "Scrapie incidence and PRNP polymorphisms: Rare small ruminant breeds of Sicily with TSE protecting genetic reservoirs",
abstract = "Background: Transmissible spongiform encephalopathies (TSE) are fatal neurodegenerative diseases of several mammalian species, including humans. In Italy, the active surveillance through rapid tests on brain stem from small ruminants started in 2002 on randomly selected samples of healthy slaughtered animals. Sampling number was proportionally related to the regional small ruminant population. Of the twenty Italian regions, Sicily has the second largest population of small ruminants which is mainly constituted by crossbreed animals (>70 {\%}). Sicily contains also three native sheep breeds Pinzirita, Comisana and Valle del Belice. Native goat breeds are Girgentana, Messinese, Argentata dell'Etna, Maltese and Rossa Mediterranea. The polymorphisms of prion protein gene (PRNP) may influence disease susceptibility and breeding programs for genetic TSE resistance are being applied in sheep. Protective alleles have been recently reported for goats also. These differ from those in sheep and may allow breeding programs in the near future. In this paper the data of active surveillance for scrapie control in general population of small ruminants in Sicily are reported together with the analysis on the polymorphism of PRNP in a number of Sicilian autochthonous breeds. The evaluation of the frequency of protective alleles is fundamental for the implementation of a TSE resistance breeding program. Results: TSE surveillance in small ruminants in Sicily showed a of total fifty seven scrapie outbreaks from 1997 to 2014 involving mainly crossbreed animals. The PRNP polymorphism analysis in autochthonous breeds showed protective allele frequencies of 30-40 {\%} ARR in sheep and 12-18 {\%} K222 in three of the four goat breeds; these breeds are distributed over limited areas of the island. Conclusion: The study on PRNP polymorphisms in Sicilian small ruminant population showed higher frequency of the protective alleles compared to most other European breeds. Our results suggest that PRNP genetic variety in Sicilian sheep and goats can be a resource for TSE resistance breeding programmes while maintaining the conservation of endangered breeds and valorisation of their typical food products.",
keywords = "Autochthonous breeds, Breeding programs, PRNP polymorphism, Scrapie surveillance, TSE",
author = "Maria Vitale and Sergio Migliore and {La Giglia}, Maria and Placido Alberti and {Di Marco Lo Presti}, Vincenzo and Langeveld, {Jan P.M.}",
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Vitale, M, Migliore, S, La Giglia, M, Alberti, P, Di Marco Lo Presti, V & Langeveld, JPM 2016, 'Scrapie incidence and PRNP polymorphisms: Rare small ruminant breeds of Sicily with TSE protecting genetic reservoirs', BMC Veterinary Research, vol. 12, no. 1, 141. https://doi.org/10.1186/s12917-016-0766-9

Scrapie incidence and PRNP polymorphisms : Rare small ruminant breeds of Sicily with TSE protecting genetic reservoirs. / Vitale, Maria; Migliore, Sergio; La Giglia, Maria; Alberti, Placido; Di Marco Lo Presti, Vincenzo; Langeveld, Jan P.M.

In: BMC Veterinary Research, Vol. 12, No. 1, 141, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Scrapie incidence and PRNP polymorphisms

T2 - Rare small ruminant breeds of Sicily with TSE protecting genetic reservoirs

AU - Vitale, Maria

AU - Migliore, Sergio

AU - La Giglia, Maria

AU - Alberti, Placido

AU - Di Marco Lo Presti, Vincenzo

AU - Langeveld, Jan P.M.

PY - 2016

Y1 - 2016

N2 - Background: Transmissible spongiform encephalopathies (TSE) are fatal neurodegenerative diseases of several mammalian species, including humans. In Italy, the active surveillance through rapid tests on brain stem from small ruminants started in 2002 on randomly selected samples of healthy slaughtered animals. Sampling number was proportionally related to the regional small ruminant population. Of the twenty Italian regions, Sicily has the second largest population of small ruminants which is mainly constituted by crossbreed animals (>70 %). Sicily contains also three native sheep breeds Pinzirita, Comisana and Valle del Belice. Native goat breeds are Girgentana, Messinese, Argentata dell'Etna, Maltese and Rossa Mediterranea. The polymorphisms of prion protein gene (PRNP) may influence disease susceptibility and breeding programs for genetic TSE resistance are being applied in sheep. Protective alleles have been recently reported for goats also. These differ from those in sheep and may allow breeding programs in the near future. In this paper the data of active surveillance for scrapie control in general population of small ruminants in Sicily are reported together with the analysis on the polymorphism of PRNP in a number of Sicilian autochthonous breeds. The evaluation of the frequency of protective alleles is fundamental for the implementation of a TSE resistance breeding program. Results: TSE surveillance in small ruminants in Sicily showed a of total fifty seven scrapie outbreaks from 1997 to 2014 involving mainly crossbreed animals. The PRNP polymorphism analysis in autochthonous breeds showed protective allele frequencies of 30-40 % ARR in sheep and 12-18 % K222 in three of the four goat breeds; these breeds are distributed over limited areas of the island. Conclusion: The study on PRNP polymorphisms in Sicilian small ruminant population showed higher frequency of the protective alleles compared to most other European breeds. Our results suggest that PRNP genetic variety in Sicilian sheep and goats can be a resource for TSE resistance breeding programmes while maintaining the conservation of endangered breeds and valorisation of their typical food products.

AB - Background: Transmissible spongiform encephalopathies (TSE) are fatal neurodegenerative diseases of several mammalian species, including humans. In Italy, the active surveillance through rapid tests on brain stem from small ruminants started in 2002 on randomly selected samples of healthy slaughtered animals. Sampling number was proportionally related to the regional small ruminant population. Of the twenty Italian regions, Sicily has the second largest population of small ruminants which is mainly constituted by crossbreed animals (>70 %). Sicily contains also three native sheep breeds Pinzirita, Comisana and Valle del Belice. Native goat breeds are Girgentana, Messinese, Argentata dell'Etna, Maltese and Rossa Mediterranea. The polymorphisms of prion protein gene (PRNP) may influence disease susceptibility and breeding programs for genetic TSE resistance are being applied in sheep. Protective alleles have been recently reported for goats also. These differ from those in sheep and may allow breeding programs in the near future. In this paper the data of active surveillance for scrapie control in general population of small ruminants in Sicily are reported together with the analysis on the polymorphism of PRNP in a number of Sicilian autochthonous breeds. The evaluation of the frequency of protective alleles is fundamental for the implementation of a TSE resistance breeding program. Results: TSE surveillance in small ruminants in Sicily showed a of total fifty seven scrapie outbreaks from 1997 to 2014 involving mainly crossbreed animals. The PRNP polymorphism analysis in autochthonous breeds showed protective allele frequencies of 30-40 % ARR in sheep and 12-18 % K222 in three of the four goat breeds; these breeds are distributed over limited areas of the island. Conclusion: The study on PRNP polymorphisms in Sicilian small ruminant population showed higher frequency of the protective alleles compared to most other European breeds. Our results suggest that PRNP genetic variety in Sicilian sheep and goats can be a resource for TSE resistance breeding programmes while maintaining the conservation of endangered breeds and valorisation of their typical food products.

KW - Autochthonous breeds

KW - Breeding programs

KW - PRNP polymorphism

KW - Scrapie surveillance

KW - TSE

U2 - 10.1186/s12917-016-0766-9

DO - 10.1186/s12917-016-0766-9

M3 - Article

VL - 12

JO - BMC Veterinary Research

JF - BMC Veterinary Research

SN - 1746-6148

IS - 1

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ER -