Scale-up aspects of photobioreactors : effects of mixing-induced light/dark cycles

M. Janssen, L. de Bresser, T. Baijens, J. Tramper, L.R. Mur, J.F.H. Snel, R.H. Wijffels

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Abstract

The green micro-algae Chlamydomonas reinhardtii and Dunaliella tertiolecta were cultivated under medium-duration square-wave light/dark cycles with a cycle time of 15 s. These cycles were used to simulate the light regime experienced by micro-algae in externally-illuminated (sunlight) air-lift loop bioreactors with internal draft tube. Biomass yield in relation to light energy was determined as g protein per mol of photons (400-700 nm). Between 600 and 1200 ?mol m^-2 s^-1 the yield at a 10/5 s light/dark cycle was equal to the yield at continuous illumination. Consequently, provided that the liquid circulation time is 15 s, a considerable dark zone seems to be allowed in the interior of air-lift loop photobioreactors (33␟/v) without loss of light utilization efficiency. However, at a 5/10 s light/dark cycle, corresponding to a 67␟/v dark zone, biomass yield decreased. Furthermore, both algae, C. reinhardtii and D. tertiolecta, responded similarly to these cycles with respect to biomass yield. This was interesting because they were reported to exhibit a different photoacclimation strategy. Finally, it was demonstrated that D. tertiolecta was much more efficient at low (average) photon flux densities (57-370 ?mol m^-2 s^-1) than at high PFDs (> 600 ?mol m^-2 s^-1) and it was shown that D. tertiolecta was cultivated at a sub-optimal temperature (20 ^°C).
LanguageEnglish
Pages225-237
JournalJournal of Applied Phycology
Volume12
DOIs
Publication statusPublished - 2000

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algae
Dunaliella tertiolecta
photoperiod
Chlamydomonas reinhardtii
microalga
biomass
air
bioreactors
photon flux density
lighting
solar radiation
bioreactor
effect
alga
duration
liquids
energy
liquid
protein
temperature

Cite this

Janssen, M. ; de Bresser, L. ; Baijens, T. ; Tramper, J. ; Mur, L.R. ; Snel, J.F.H. ; Wijffels, R.H. / Scale-up aspects of photobioreactors : effects of mixing-induced light/dark cycles. In: Journal of Applied Phycology. 2000 ; Vol. 12. pp. 225-237.
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abstract = "The green micro-algae Chlamydomonas reinhardtii and Dunaliella tertiolecta were cultivated under medium-duration square-wave light/dark cycles with a cycle time of 15 s. These cycles were used to simulate the light regime experienced by micro-algae in externally-illuminated (sunlight) air-lift loop bioreactors with internal draft tube. Biomass yield in relation to light energy was determined as g protein per mol of photons (400-700 nm). Between 600 and 1200 ?mol m^-2 s^-1 the yield at a 10/5 s light/dark cycle was equal to the yield at continuous illumination. Consequently, provided that the liquid circulation time is 15 s, a considerable dark zone seems to be allowed in the interior of air-lift loop photobioreactors (33␟/v) without loss of light utilization efficiency. However, at a 5/10 s light/dark cycle, corresponding to a 67␟/v dark zone, biomass yield decreased. Furthermore, both algae, C. reinhardtii and D. tertiolecta, responded similarly to these cycles with respect to biomass yield. This was interesting because they were reported to exhibit a different photoacclimation strategy. Finally, it was demonstrated that D. tertiolecta was much more efficient at low (average) photon flux densities (57-370 ?mol m^-2 s^-1) than at high PFDs (> 600 ?mol m^-2 s^-1) and it was shown that D. tertiolecta was cultivated at a sub-optimal temperature (20 ^°C).",
author = "M. Janssen and {de Bresser}, L. and T. Baijens and J. Tramper and L.R. Mur and J.F.H. Snel and R.H. Wijffels",
year = "2000",
doi = "10.1023/A:1008151526680",
language = "English",
volume = "12",
pages = "225--237",
journal = "Journal of Applied Phycology",
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Scale-up aspects of photobioreactors : effects of mixing-induced light/dark cycles. / Janssen, M.; de Bresser, L.; Baijens, T.; Tramper, J.; Mur, L.R.; Snel, J.F.H.; Wijffels, R.H.

In: Journal of Applied Phycology, Vol. 12, 2000, p. 225-237.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Scale-up aspects of photobioreactors : effects of mixing-induced light/dark cycles

AU - Janssen, M.

AU - de Bresser, L.

AU - Baijens, T.

AU - Tramper, J.

AU - Mur, L.R.

AU - Snel, J.F.H.

AU - Wijffels, R.H.

PY - 2000

Y1 - 2000

N2 - The green micro-algae Chlamydomonas reinhardtii and Dunaliella tertiolecta were cultivated under medium-duration square-wave light/dark cycles with a cycle time of 15 s. These cycles were used to simulate the light regime experienced by micro-algae in externally-illuminated (sunlight) air-lift loop bioreactors with internal draft tube. Biomass yield in relation to light energy was determined as g protein per mol of photons (400-700 nm). Between 600 and 1200 ?mol m^-2 s^-1 the yield at a 10/5 s light/dark cycle was equal to the yield at continuous illumination. Consequently, provided that the liquid circulation time is 15 s, a considerable dark zone seems to be allowed in the interior of air-lift loop photobioreactors (33␟/v) without loss of light utilization efficiency. However, at a 5/10 s light/dark cycle, corresponding to a 67␟/v dark zone, biomass yield decreased. Furthermore, both algae, C. reinhardtii and D. tertiolecta, responded similarly to these cycles with respect to biomass yield. This was interesting because they were reported to exhibit a different photoacclimation strategy. Finally, it was demonstrated that D. tertiolecta was much more efficient at low (average) photon flux densities (57-370 ?mol m^-2 s^-1) than at high PFDs (> 600 ?mol m^-2 s^-1) and it was shown that D. tertiolecta was cultivated at a sub-optimal temperature (20 ^°C).

AB - The green micro-algae Chlamydomonas reinhardtii and Dunaliella tertiolecta were cultivated under medium-duration square-wave light/dark cycles with a cycle time of 15 s. These cycles were used to simulate the light regime experienced by micro-algae in externally-illuminated (sunlight) air-lift loop bioreactors with internal draft tube. Biomass yield in relation to light energy was determined as g protein per mol of photons (400-700 nm). Between 600 and 1200 ?mol m^-2 s^-1 the yield at a 10/5 s light/dark cycle was equal to the yield at continuous illumination. Consequently, provided that the liquid circulation time is 15 s, a considerable dark zone seems to be allowed in the interior of air-lift loop photobioreactors (33␟/v) without loss of light utilization efficiency. However, at a 5/10 s light/dark cycle, corresponding to a 67␟/v dark zone, biomass yield decreased. Furthermore, both algae, C. reinhardtii and D. tertiolecta, responded similarly to these cycles with respect to biomass yield. This was interesting because they were reported to exhibit a different photoacclimation strategy. Finally, it was demonstrated that D. tertiolecta was much more efficient at low (average) photon flux densities (57-370 ?mol m^-2 s^-1) than at high PFDs (> 600 ?mol m^-2 s^-1) and it was shown that D. tertiolecta was cultivated at a sub-optimal temperature (20 ^°C).

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M3 - Article

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JF - Journal of Applied Phycology

SN - 0921-8971

ER -