Projects per year
Abstract
Currently, Chinese Hamster Ovary (CHO) cells are the main expression system used for the production of therapeutic monoclonal antibodies (mAbs). The scale-up of a CHO cell culture process is an important step during the upstream development. A representative lab-scale bioreactor (milli-litres to litres volume) for the production-scale bioreactor (kilo-litres volume) is important for a robust process scale-up. In this thesis, a miniature bioreactor system (ambr® 15, a 15 mL volume system) is used as a scale-down model for a 10 L bench-scale bioreactor system. A thorough characterization of the physiology of the CHO cells, in terms of metabolism and transcriptome, is carried out for the fed-batch process run at both the ambr® 15 and the 10 L scale. The characteristic cell physiology is used in conjugation with the engineering first principles to understand the differences between scales for a cell culture process. The result of this thesis gives a better understanding of cell physiology in fed-batch cultures and on the scale-down of these processes. This knowledge can be used to increase the efficiency of process development and thus shorten the time-to-market for the biopharmaceutical products.
Original language | English |
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Qualification | Doctor of Philosophy |
Awarding Institution |
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Supervisors/Advisors |
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Award date | 29 May 2018 |
Place of Publication | Wageningen |
Publisher | |
Print ISBNs | 9789463438438 |
DOIs | |
Publication status | Published - 29 May 2018 |
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Dive into the research topics of 'Scale-down of CHO cell fed-batch cultures'. Together they form a unique fingerprint.Projects
- 1 Finished
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Animal cell cultivation and screening system (ACCESS)
Pan, X. (PhD candidate), Wijffels, R. (Promotor) & Martens, D. (Co-promotor)
1/10/12 → 29/05/18
Project: PhD