Sandwich immunoassays for the determination of peanut and hazelnut traces in foods

M. Kiening, R. Niessner, S. Baumgartner, E. Drs, R. Krska, M.G.E.G. Bremer, V. Tomkies, P. Reece, C. Danks, U. Immer, M.G. Wellner

    Research output: Contribution to journalArticleAcademicpeer-review

    55 Citations (Scopus)

    Abstract

    People suffering from food allergies are dependent on accurate food labeling, as an avoidance diet is the only effective countermeasure. Even a small amount of allergenic protein can trigger severe reactions in highly sensitized patients. Therefore, sensitive and reliable tests are needed to detect potential cross-contamination. In this paper two fast sandwich immunoassays are described for the determination of peanut (Arachis hypogaea) and hazelnut (Corylus avellana) traces in complex food matrices. Mouse monoclonal antibodies were used as capture antibodies, and labeled rabbit polyclonal antibodies were used as detection antibodies in both assays. The assay time was 30 min in total, and cross-reactivities against a variety of fruits and seeds were found to be in the low 10-4% (ppm) level or in some cases not detectable. The recoveries in all tested food matrices ranged from 86 to 127%, and the limits of detection were in the range of 0.2-1.2 mg/kg (ppm) in food for both peanut and hazelnut, respectively
    Original languageEnglish
    Pages (from-to)3321-3327
    JournalJournal of Agricultural and Food Chemistry
    Volume53
    Issue number9
    DOIs
    Publication statusPublished - 2005

    Keywords

    • tree nut allergy
    • dial telephone survey
    • elisa
    • prevalence
    • matrices
    • quantification
    • proteins
    • kits

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