Salivary biomarkers associated with perceived satiety and body mass in humans

L.F. Harthoorn; R.G. Schipper; A. Loof; W. van Heerde; J.H.G. Cordewener; A.H.P. America; P.F.G. Vereijken; E. Dransfield

doi:10.1002/prca.200700448

Salivary biomarkers associated with perceived satiety and body mass in humans

L.F. Harthoorn
, R.G. Schipper
, A. Loof
, W. van Heerde
, J.H.G. Cordewener
, A.H.P. America
, P.F.G. Vereijken
, E. Dransfield

VLAG

Research output: Contribution to journal › Article › Academic › peer-review

17 Citations (Scopus)

Abstract

Regulation of food intake and energy homeostasis is controlled by a delicate balancing of numerous central and peripheral factors, including circulating peptide hormones. This study investigated the proteome of saliva using SELDI-TOF-MS in relation to satiety and body mass index (BMI) in humans. Within a longitudinal test session, 18 subjects were exposed to a lunch-induced hunger-satiety shift, while every 15 min collecting their whole saliva and rating their hunger and satiety. Saliva was analysed by SELDI-TOF-MS using IMAC arrays with a chromatographic copper surface (IMAC-Cu). From all subjects and time points measured, peptide and protein profiles showed 190 common peaks. Their interrelationships show that 37% of the variation was accounted for in one dimension. About 30 means had a strong association (0.70

Original language	English
Pages (from-to)	1637-1650
Journal	Proteomics Clinical Applications
Volume	1
Issue number	12
DOIs	https://doi.org/10.1002/prca.200700448
Publication status	Published - 2007

Keywords

2-dimensional gel-electrophoresis
human whole saliva
proteomic analysis
food-intake
ionization-time
spectrometry
ghrelin
identification
insulin
histatins

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10.1002/prca.200700448

Cite this

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title = "Salivary biomarkers associated with perceived satiety and body mass in humans",

abstract = "Regulation of food intake and energy homeostasis is controlled by a delicate balancing of numerous central and peripheral factors, including circulating peptide hormones. This study investigated the proteome of saliva using SELDI-TOF-MS in relation to satiety and body mass index (BMI) in humans. Within a longitudinal test session, 18 subjects were exposed to a lunch-induced hunger-satiety shift, while every 15 min collecting their whole saliva and rating their hunger and satiety. Saliva was analysed by SELDI-TOF-MS using IMAC arrays with a chromatographic copper surface (IMAC-Cu). From all subjects and time points measured, peptide and protein profiles showed 190 common peaks. Their interrelationships show that 37\% of the variation was accounted for in one dimension. About 30 means had a strong association (0.70",

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author = "L.F. Harthoorn and R.G. Schipper and A. Loof and \{van Heerde\}, W. and J.H.G. Cordewener and A.H.P. America and P.F.G. Vereijken and E. Dransfield",

year = "2007",

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AU - Harthoorn, L.F.

AU - Schipper, R.G.

AU - Loof, A.

AU - van Heerde, W.

AU - Cordewener, J.H.G.

AU - America, A.H.P.

AU - Vereijken, P.F.G.

AU - Dransfield, E.

PY - 2007

Y1 - 2007

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AB - Regulation of food intake and energy homeostasis is controlled by a delicate balancing of numerous central and peripheral factors, including circulating peptide hormones. This study investigated the proteome of saliva using SELDI-TOF-MS in relation to satiety and body mass index (BMI) in humans. Within a longitudinal test session, 18 subjects were exposed to a lunch-induced hunger-satiety shift, while every 15 min collecting their whole saliva and rating their hunger and satiety. Saliva was analysed by SELDI-TOF-MS using IMAC arrays with a chromatographic copper surface (IMAC-Cu). From all subjects and time points measured, peptide and protein profiles showed 190 common peaks. Their interrelationships show that 37% of the variation was accounted for in one dimension. About 30 means had a strong association (0.70

KW - 2-dimensional gel-electrophoresis

KW - human whole saliva

KW - proteomic analysis

KW - food-intake

KW - ionization-time

KW - spectrometry

KW - ghrelin

KW - identification

KW - insulin

KW - histatins

U2 - 10.1002/prca.200700448

DO - 10.1002/prca.200700448

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JO - Proteomics Clinical Applications

JF - Proteomics Clinical Applications

IS - 12

ER -

Salivary biomarkers associated with perceived satiety and body mass in humans

Abstract

Keywords

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