Salicylic acid can reduce infection symptoms caused by Dickeya solani in tissue culture grown potato (Solanum tuberosum L.) plants

Robert Czajkowski*, Jan M. van der Wolf, Aleksandra Krolicka, Zofia Ozymko, Magdalena Narajczyk, Natalia Kaczynska, Ewa Lojkowska

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

The potential for control of Dickeya solani infections in potato by elicitation of in vitro grown potato plants with salicylic acid (SA) was investigated by selective plating and confocal laser scanning macroscopy (CLSM). In replicated experiments potato plants grown on medium supplemented with 25 or 50 μM of SA were evaluated for the phytotoxic effects. Potato plants grown on medium supplemented with SA and inoculated with GFP-tagged D. solani were investigated for blackleg development and colonization of potato plants by the bacteria. Three days after inoculation, colonization of roots by D. solani was observed in 100 % control plants grown on medium without SA but not in plants grown on medium supplemented with 50 μM SA. After 14 days, 100 % of control plants showed severe disease symptoms, whereas plants grown on medium supplemented with 50 μM SA and inoculated with bacteria did not express any symptoms. After 14 days bacteria were found inside 100 % stems of control plants in densities of ca. 103–104 cfu g−1 and inside ca. 10–15 % stems of plants treated with 50 μM SA in densities similar to these in the control plants. The GFP-tagged bacteria were macroscopically detected on the surface of the roots of control plants but not on the surface of the plants treated with 50 μM SA 14 days after inoculation. The implications of SA treatments on plant fitness and disease development are discussed.

Original languageEnglish
Pages (from-to)545-558
Number of pages14
JournalEuropean Journal of Plant Pathology
Volume141
Issue number3
DOIs
Publication statusPublished - Mar 2015

Keywords

  • Biological control
  • Blackleg
  • Dose effect
  • Elicitation
  • Erwinia chrysanthemi
  • Induced resistance
  • SAR

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