Regular monitoring of circulating serum β-hydroxybutyrate is vital for dairy cattle health management. Herein, an electrochemical biosensor platform for rapid detection of β-hydroxybutyrate is described that is simple to operate. The biosensor platform is constructed by integrating the covalently functionalized ruthenium (II)-graphene oxide/NAD+ composite with subsequent modification of enzyme, 3-hydroxybutyrate dehydrogenase. As-functionalized biosensor platform display enhanced redox behavior at the interface with a current sensitivity of 22 ± 2.51 μA mM-1. Immobilization of enzyme 3-hydroxybutyrate dehydrogenase makes the sensor platform selective to β-hydroxybutyrate, which induces the concentration dependent amperometric response suitable for field-deployable application. Further, practical applicability of the proposed biosensor platform is tested with serum samples of various β-hydroxybutyrate concentrations. In addition to better selectivity, the demonstrated enzyme integrated hybrid nanocomposite biosensor exhibit rapid response time (less than 1 min), providing a promising approach for on-farm diagnostics.
- 3-hydroxybutyrate dehydrogenase
- BHBA detection
- Biosensor platform
- Ruthenium (II)-graphene oxide