TY - JOUR
T1 - Rotylenchus wimbii n. sp. (Nematoda
T2 - Hoplolaimidae) associated with finger millet in Kenya
AU - Singh, Phougeishangbam Rolish
AU - Karssen, Gerrit
AU - Gitau, Kelvin
AU - Wanjau, Cecilia
AU - Couvreur, Marjolein
AU - Pili, Njira Njira
AU - Gheysen, Godelieve
AU - Bert, Wim
PY - 2021/2/22
Y1 - 2021/2/22
N2 - Rotylenchus wimbii n. sp. was found associated with finger millet in Kenya and is described based on light microscopy, scanning electron microscopy, and molecular information. Sequence analysis was performed on ITS, 18S, and D2-D3 of 28S of ribosomal DNA and COI of mitochondrial DNA. This new species is characterized by a moderate female body size of 0.6 to 0.8mm, a continuous hemispherical lip region with four annuli, 3 to 4 irregular blocks on the basal lip annule, absence of longitudinal cuticular striations in anterior region, four lateral lines forming three equal bands which are areolated mainly at pharynx level, a robust stylet of 23 to 27µm of which 45 to 53% is cone part, and with rounded to sometimes indented knobs, a secretory-excretory pore around level of pharyngo-intestinal junction, didelphic-amphidelphic reproductive system, vulva without distinct epiptygma, indistinct to empty spermatheca, tail usually truncated with 5 to 9 annuli, phasmids located at 7 to 17 annuli anterior to anus, and absence of males. Molecular phylogenies, in combination with species delimitation, supported the distinctiveness of Rotylenchus wimbii n. sp. and revealed some mislabeled Rotylenchus brevicaudatus sequences in GenBank.
AB - Rotylenchus wimbii n. sp. was found associated with finger millet in Kenya and is described based on light microscopy, scanning electron microscopy, and molecular information. Sequence analysis was performed on ITS, 18S, and D2-D3 of 28S of ribosomal DNA and COI of mitochondrial DNA. This new species is characterized by a moderate female body size of 0.6 to 0.8mm, a continuous hemispherical lip region with four annuli, 3 to 4 irregular blocks on the basal lip annule, absence of longitudinal cuticular striations in anterior region, four lateral lines forming three equal bands which are areolated mainly at pharynx level, a robust stylet of 23 to 27µm of which 45 to 53% is cone part, and with rounded to sometimes indented knobs, a secretory-excretory pore around level of pharyngo-intestinal junction, didelphic-amphidelphic reproductive system, vulva without distinct epiptygma, indistinct to empty spermatheca, tail usually truncated with 5 to 9 annuli, phasmids located at 7 to 17 annuli anterior to anus, and absence of males. Molecular phylogenies, in combination with species delimitation, supported the distinctiveness of Rotylenchus wimbii n. sp. and revealed some mislabeled Rotylenchus brevicaudatus sequences in GenBank.
KW - 18S
KW - 28S
KW - COI
KW - Finger millet
KW - ITS
KW - Kenya
KW - Morphometrics
KW - New species
KW - Phylogeny
KW - Plant-parasitic nematodes
KW - Rotylenchus
KW - SEM
KW - Systematics
KW - Taxonomy
U2 - 10.21307/jofnem-2021-016
DO - 10.21307/jofnem-2021-016
M3 - Article
AN - SCOPUS:85103506470
SN - 0022-300X
VL - 53
JO - Journal of Nematology
JF - Journal of Nematology
M1 - e2021-016
ER -