Rotavirus vaccine response correlates with the infant gut microbiota composition in Pakistan

Vanessa C. Harris, Asad Ali, Susana Fuentes, Katri Korpela, Momin Kazi, Jacqueline Tate, Umesh Parashar, W.J. Wiersinga, Carlo Giaquinto, Carolina de Weerth, Willem M. de Vos

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16 Citations (Scopus)

Abstract

Rotavirus (RV) is the leading cause of diarrhea-related death in children worldwide and ninety-five percent of rotavirus deaths occur in Africa and Asia. Rotavirus vaccines (RVV) can dramatically reduce RV deaths, but have low efficacy in low-income settings where they are most needed. The intestinal microbiome may contribute to this decreased RVV efficacy. This pilot study hypothesizes that infants' intestinal microbiota composition correlates with RVV immune responses and that RVV responders have different gut microbiota as compared to non-responders. We conducted a nested, matched case-control study comparing the pre-vaccination intestinal microbiota composition between 10 6-week old Pakistani RVV-responders, 10 6-week old Pakistani RVV non-responders, and 10 healthy Dutch infants.  RVV response was defined as an Immunoglobulin A of ≥20 IU/mL following Rotarix™(RV1) vaccination in an infant with a pre-vaccination IgA<20. Infants were matched in a 1:1 ratio using ranked variables: RV1 dosing schedule (6/10/14; 6/10; or 10/14 weeks), RV season, delivery mode, delivery place, breastfeeding practices, age and gender. Fecal microbiota analysis was performed using a highly reproducible phylogenetic microarray. RV1 response correlated with a higher relative abundance of bacteria belonging to Clostridium cluster XI and Proteobacteria, including bacteria related to Serratia and Escherichia coli. Remarkably, abundance of these Proteobacteria was also significantly higher in Dutch infants when compared to RV1-non-responders in Pakistan. This small but carefully matched study showed the intestinal microbiota composition to correlate with RV1 seroconversion in Pakistan infants, identifying signatures shared with healthy Dutch infants.

LanguageEnglish
Pages93-101
JournalGut Microbes
Volume9
Issue number2
Early online date11 Sep 2017
DOIs
Publication statusPublished - Mar 2018

Fingerprint

Rotavirus Vaccines
Pakistan
Rotavirus
Proteobacteria
Vaccination
Immunoglobulin A
Case-Control Studies
Bacteria
Serratia
Clostridium
Microbiota
Gastrointestinal Microbiome
Breast Feeding
Diarrhea
Appointments and Schedules
Escherichia coli

Keywords

  • intestinal microbes
  • rotavirus vaccine
  • seroconversion
  • vaccine immunogenicity

Cite this

Harris, Vanessa C. ; Ali, Asad ; Fuentes, Susana ; Korpela, Katri ; Kazi, Momin ; Tate, Jacqueline ; Parashar, Umesh ; Wiersinga, W.J. ; Giaquinto, Carlo ; de Weerth, Carolina ; de Vos, Willem M. / Rotavirus vaccine response correlates with the infant gut microbiota composition in Pakistan. In: Gut Microbes. 2018 ; Vol. 9, No. 2. pp. 93-101.
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title = "Rotavirus vaccine response correlates with the infant gut microbiota composition in Pakistan",
abstract = "Rotavirus (RV) is the leading cause of diarrhea-related death in children worldwide and ninety-five percent of rotavirus deaths occur in Africa and Asia. Rotavirus vaccines (RVV) can dramatically reduce RV deaths, but have low efficacy in low-income settings where they are most needed. The intestinal microbiome may contribute to this decreased RVV efficacy. This pilot study hypothesizes that infants' intestinal microbiota composition correlates with RVV immune responses and that RVV responders have different gut microbiota as compared to non-responders. We conducted a nested, matched case-control study comparing the pre-vaccination intestinal microbiota composition between 10 6-week old Pakistani RVV-responders, 10 6-week old Pakistani RVV non-responders, and 10 healthy Dutch infants.  RVV response was defined as an Immunoglobulin A of ≥20 IU/mL following Rotarix™(RV1) vaccination in an infant with a pre-vaccination IgA<20. Infants were matched in a 1:1 ratio using ranked variables: RV1 dosing schedule (6/10/14; 6/10; or 10/14 weeks), RV season, delivery mode, delivery place, breastfeeding practices, age and gender. Fecal microbiota analysis was performed using a highly reproducible phylogenetic microarray. RV1 response correlated with a higher relative abundance of bacteria belonging to Clostridium cluster XI and Proteobacteria, including bacteria related to Serratia and Escherichia coli. Remarkably, abundance of these Proteobacteria was also significantly higher in Dutch infants when compared to RV1-non-responders in Pakistan. This small but carefully matched study showed the intestinal microbiota composition to correlate with RV1 seroconversion in Pakistan infants, identifying signatures shared with healthy Dutch infants.",
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Harris, VC, Ali, A, Fuentes, S, Korpela, K, Kazi, M, Tate, J, Parashar, U, Wiersinga, WJ, Giaquinto, C, de Weerth, C & de Vos, WM 2018, 'Rotavirus vaccine response correlates with the infant gut microbiota composition in Pakistan', Gut Microbes, vol. 9, no. 2, pp. 93-101. https://doi.org/10.1080/19490976.2017.1376162

Rotavirus vaccine response correlates with the infant gut microbiota composition in Pakistan. / Harris, Vanessa C.; Ali, Asad; Fuentes, Susana; Korpela, Katri; Kazi, Momin; Tate, Jacqueline; Parashar, Umesh; Wiersinga, W.J.; Giaquinto, Carlo; de Weerth, Carolina; de Vos, Willem M.

In: Gut Microbes, Vol. 9, No. 2, 03.2018, p. 93-101.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Kazi, Momin

AU - Tate, Jacqueline

AU - Parashar, Umesh

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