Root branching under high salinity requires auxin-independent modulation of LATERAL ORGAN BOUNDARY DOMAIN 16 function

Yanxia Zhang; Yiyun Li; Thijs De zeeuw; Kilian Duijts; Dorota Kawa; Jasper Lamers; Kristina Munzert; Hongfei Li; Yutao Zou; Jessica Meyer; Jinxuan Yan; Francel Verstappen; Yixuan Wang; Tom Gijsberts; Jielin Wang; Nora Gigli-Bisceglia; Timo Engelsdorf; Aalt van Dijk; Christa Testerink

doi:10.1093/plcell/koad317

Root branching under high salinity requires auxin-independent modulation of LATERAL ORGAN BOUNDARY DOMAIN 16 function

Yanxia Zhang^*, Yiyun Li, Thijs De zeeuw, Kilian Duijts, Dorota Kawa, Jasper Lamers, Kristina Munzert, Hongfei Li, Yutao Zou, Jessica Meyer, Jinxuan Yan, Francel Verstappen, Yixuan Wang, Tom Gijsberts, Jielin Wang, Nora Gigli-Bisceglia, Timo Engelsdorf, Aalt van Dijk, Christa Testerink^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

Salinity stress constrains lateral root (LR) growth and severely affects plant growth. Auxin signaling regulates LR formation, but the molecular mechanism by which salinity affects root auxin signaling and whether salt induces other pathways that regulate LR development remains unknown. In Arabidopsis thaliana, the auxin-regulated transcription factor LATERAL ORGAN BOUNDARY DOMAIN 16 (LBD16) is an essential player in LR development under control conditions. Here, we show that under high-salt conditions, an alternative pathway regulates LBD16 expression. Salt represses auxin signaling but, in parallel, activates ZINC FINGER OF ARABIDOPSIS THALIANA 6 (ZAT6), a transcriptional activator of LBD16. ZAT6 activates LBD16 expression, thus contributing to downstream cell wall remodeling and promoting LR development under high-salt conditions. Our study thus shows that the integration of auxin-dependent repressive and salt-activated auxin-independent pathways converging on LBD16 modulates root branching under high-salt conditions.

Original language	English
Article number	koad317
Pages (from-to)	899-918
Journal	The Plant Cell
Volume	36
Issue number	4
Early online date	8 Dec 2023
DOIs	https://doi.org/10.1093/plcell/koad317
Publication status	Published - 2024

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https://edepot.wur.nl/649773Licence: CC BY

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Zhang, Y., Li, Y., De zeeuw, T., Duijts, K., Kawa, D., Lamers, J., Munzert, K., Li, H., Zou, Y., Meyer, J., Yan, J., Verstappen, F., Wang, Y., Gijsberts, T., Wang, J., Gigli-Bisceglia, N., Engelsdorf, T., van Dijk, A., & Testerink, C. (2024). Root branching under high salinity requires auxin-independent modulation of LATERAL ORGAN BOUNDARY DOMAIN 16 function. The Plant Cell, 36(4), 899-918. Article koad317. https://doi.org/10.1093/plcell/koad317

@article{2a1ea6fecf6243828361a6c217248bcc,

title = "Root branching under high salinity requires auxin-independent modulation of LATERAL ORGAN BOUNDARY DOMAIN 16 function",

abstract = "Salinity stress constrains lateral root (LR) growth and severely affects plant growth. Auxin signaling regulates LR formation, but the molecular mechanism by which salinity affects root auxin signaling and whether salt induces other pathways that regulate LR development remains unknown. In Arabidopsis thaliana, the auxin-regulated transcription factor LATERAL ORGAN BOUNDARY DOMAIN 16 (LBD16) is an essential player in LR development under control conditions. Here, we show that under high-salt conditions, an alternative pathway regulates LBD16 expression. Salt represses auxin signaling but, in parallel, activates ZINC FINGER OF ARABIDOPSIS THALIANA 6 (ZAT6), a transcriptional activator of LBD16. ZAT6 activates LBD16 expression, thus contributing to downstream cell wall remodeling and promoting LR development under high-salt conditions. Our study thus shows that the integration of auxin-dependent repressive and salt-activated auxin-independent pathways converging on LBD16 modulates root branching under high-salt conditions.",

author = "Yanxia Zhang and Yiyun Li and {De zeeuw}, Thijs and Kilian Duijts and Dorota Kawa and Jasper Lamers and Kristina Munzert and Hongfei Li and Yutao Zou and Jessica Meyer and Jinxuan Yan and Francel Verstappen and Yixuan Wang and Tom Gijsberts and Jielin Wang and Nora Gigli-Bisceglia and Timo Engelsdorf and {van Dijk}, Aalt and Christa Testerink",

year = "2024",

doi = "10.1093/plcell/koad317",

language = "English",

volume = "36",

pages = "899--918",

journal = "The Plant Cell",

issn = "1040-4651",

publisher = "American Society of Plant Biologists",

number = "4",

}

Zhang, Y, Li, Y , De zeeuw, T , Duijts, K, Kawa, D, Lamers, J, Munzert, K, Li, H, Zou, Y, Meyer, J, Yan, J, Verstappen, F, Wang, Y, Gijsberts, T , Wang, J, Gigli-Bisceglia, N, Engelsdorf, T, van Dijk, A & Testerink, C 2024, 'Root branching under high salinity requires auxin-independent modulation of LATERAL ORGAN BOUNDARY DOMAIN 16 function', The Plant Cell, vol. 36, no. 4, koad317, pp. 899-918. https://doi.org/10.1093/plcell/koad317

TY - JOUR

T1 - Root branching under high salinity requires auxin-independent modulation of LATERAL ORGAN BOUNDARY DOMAIN 16 function

AU - Zhang, Yanxia

AU - Li, Yiyun

AU - De zeeuw, Thijs

AU - Duijts, Kilian

AU - Kawa, Dorota

AU - Lamers, Jasper

AU - Munzert, Kristina

AU - Li, Hongfei

AU - Zou, Yutao

AU - Meyer, Jessica

AU - Yan, Jinxuan

AU - Verstappen, Francel

AU - Wang, Yixuan

AU - Gijsberts, Tom

AU - Wang, Jielin

AU - Gigli-Bisceglia, Nora

AU - Engelsdorf, Timo

AU - van Dijk, Aalt

AU - Testerink, Christa

PY - 2024

Y1 - 2024

N2 - Salinity stress constrains lateral root (LR) growth and severely affects plant growth. Auxin signaling regulates LR formation, but the molecular mechanism by which salinity affects root auxin signaling and whether salt induces other pathways that regulate LR development remains unknown. In Arabidopsis thaliana, the auxin-regulated transcription factor LATERAL ORGAN BOUNDARY DOMAIN 16 (LBD16) is an essential player in LR development under control conditions. Here, we show that under high-salt conditions, an alternative pathway regulates LBD16 expression. Salt represses auxin signaling but, in parallel, activates ZINC FINGER OF ARABIDOPSIS THALIANA 6 (ZAT6), a transcriptional activator of LBD16. ZAT6 activates LBD16 expression, thus contributing to downstream cell wall remodeling and promoting LR development under high-salt conditions. Our study thus shows that the integration of auxin-dependent repressive and salt-activated auxin-independent pathways converging on LBD16 modulates root branching under high-salt conditions.

AB - Salinity stress constrains lateral root (LR) growth and severely affects plant growth. Auxin signaling regulates LR formation, but the molecular mechanism by which salinity affects root auxin signaling and whether salt induces other pathways that regulate LR development remains unknown. In Arabidopsis thaliana, the auxin-regulated transcription factor LATERAL ORGAN BOUNDARY DOMAIN 16 (LBD16) is an essential player in LR development under control conditions. Here, we show that under high-salt conditions, an alternative pathway regulates LBD16 expression. Salt represses auxin signaling but, in parallel, activates ZINC FINGER OF ARABIDOPSIS THALIANA 6 (ZAT6), a transcriptional activator of LBD16. ZAT6 activates LBD16 expression, thus contributing to downstream cell wall remodeling and promoting LR development under high-salt conditions. Our study thus shows that the integration of auxin-dependent repressive and salt-activated auxin-independent pathways converging on LBD16 modulates root branching under high-salt conditions.

U2 - 10.1093/plcell/koad317

DO - 10.1093/plcell/koad317

M3 - Article

SN - 1040-4651

VL - 36

SP - 899

EP - 918

JO - The Plant Cell

JF - The Plant Cell

IS - 4

M1 - koad317

ER -

Root branching under high salinity requires auxin-independent modulation of LATERAL ORGAN BOUNDARY DOMAIN 16 function

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