Root branching toward water involves posttranslational modification of transcription factor ARF7

Beatriz Orosa-Puente, Nicola Leftley, Daniel von Wangenheim, Jason Banda, Anjil K. Srivastava, Kristine Hill, Jekaterina Truskina, Rahul Bhosale, Emily Morris, Moumita Srivastava, Britta Kümpers, Tatsuaki Goh, Hidehiro Fukaki, Joop E.M. Vermeer, Teva Vernoux, José R. Dinneny, Andrew P. French, Anthony Bishopp, Ari Sadanandom*, Malcolm J. Bennett

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)

Abstract

Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water by using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells. This differential expression pattern is regulated by posttranslational modification of ARF7 with the small ubiquitin-like modifier (SUMO) protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA (indole-3-acetic acid) repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.

Original languageEnglish
Pages (from-to)1407-1410
JournalScience
Volume362
Issue number6421
DOIs
Publication statusPublished - 21 Dec 2018

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Sumoylation
Post Translational Protein Processing
Indoleacetic Acids
Transcription Factors
Water
Ubiquitins
Repressor Proteins
Ubiquitin
Soil
DNA
Genes

Cite this

Orosa-Puente, B., Leftley, N., von Wangenheim, D., Banda, J., Srivastava, A. K., Hill, K., ... Bennett, M. J. (2018). Root branching toward water involves posttranslational modification of transcription factor ARF7. Science, 362(6421), 1407-1410. https://doi.org/10.1126/science.aau3956
Orosa-Puente, Beatriz ; Leftley, Nicola ; von Wangenheim, Daniel ; Banda, Jason ; Srivastava, Anjil K. ; Hill, Kristine ; Truskina, Jekaterina ; Bhosale, Rahul ; Morris, Emily ; Srivastava, Moumita ; Kümpers, Britta ; Goh, Tatsuaki ; Fukaki, Hidehiro ; Vermeer, Joop E.M. ; Vernoux, Teva ; Dinneny, José R. ; French, Andrew P. ; Bishopp, Anthony ; Sadanandom, Ari ; Bennett, Malcolm J. / Root branching toward water involves posttranslational modification of transcription factor ARF7. In: Science. 2018 ; Vol. 362, No. 6421. pp. 1407-1410.
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title = "Root branching toward water involves posttranslational modification of transcription factor ARF7",
abstract = "Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water by using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells. This differential expression pattern is regulated by posttranslational modification of ARF7 with the small ubiquitin-like modifier (SUMO) protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA (indole-3-acetic acid) repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.",
author = "Beatriz Orosa-Puente and Nicola Leftley and {von Wangenheim}, Daniel and Jason Banda and Srivastava, {Anjil K.} and Kristine Hill and Jekaterina Truskina and Rahul Bhosale and Emily Morris and Moumita Srivastava and Britta K{\"u}mpers and Tatsuaki Goh and Hidehiro Fukaki and Vermeer, {Joop E.M.} and Teva Vernoux and Dinneny, {Jos{\'e} R.} and French, {Andrew P.} and Anthony Bishopp and Ari Sadanandom and Bennett, {Malcolm J.}",
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month = "12",
day = "21",
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language = "English",
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Orosa-Puente, B, Leftley, N, von Wangenheim, D, Banda, J, Srivastava, AK, Hill, K, Truskina, J, Bhosale, R, Morris, E, Srivastava, M, Kümpers, B, Goh, T, Fukaki, H, Vermeer, JEM, Vernoux, T, Dinneny, JR, French, AP, Bishopp, A, Sadanandom, A & Bennett, MJ 2018, 'Root branching toward water involves posttranslational modification of transcription factor ARF7', Science, vol. 362, no. 6421, pp. 1407-1410. https://doi.org/10.1126/science.aau3956

Root branching toward water involves posttranslational modification of transcription factor ARF7. / Orosa-Puente, Beatriz; Leftley, Nicola; von Wangenheim, Daniel; Banda, Jason; Srivastava, Anjil K.; Hill, Kristine; Truskina, Jekaterina; Bhosale, Rahul; Morris, Emily; Srivastava, Moumita; Kümpers, Britta; Goh, Tatsuaki; Fukaki, Hidehiro; Vermeer, Joop E.M.; Vernoux, Teva; Dinneny, José R.; French, Andrew P.; Bishopp, Anthony; Sadanandom, Ari; Bennett, Malcolm J.

In: Science, Vol. 362, No. 6421, 21.12.2018, p. 1407-1410.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Root branching toward water involves posttranslational modification of transcription factor ARF7

AU - Orosa-Puente, Beatriz

AU - Leftley, Nicola

AU - von Wangenheim, Daniel

AU - Banda, Jason

AU - Srivastava, Anjil K.

AU - Hill, Kristine

AU - Truskina, Jekaterina

AU - Bhosale, Rahul

AU - Morris, Emily

AU - Srivastava, Moumita

AU - Kümpers, Britta

AU - Goh, Tatsuaki

AU - Fukaki, Hidehiro

AU - Vermeer, Joop E.M.

AU - Vernoux, Teva

AU - Dinneny, José R.

AU - French, Andrew P.

AU - Bishopp, Anthony

AU - Sadanandom, Ari

AU - Bennett, Malcolm J.

PY - 2018/12/21

Y1 - 2018/12/21

N2 - Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water by using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells. This differential expression pattern is regulated by posttranslational modification of ARF7 with the small ubiquitin-like modifier (SUMO) protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA (indole-3-acetic acid) repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.

AB - Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water by using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells. This differential expression pattern is regulated by posttranslational modification of ARF7 with the small ubiquitin-like modifier (SUMO) protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA (indole-3-acetic acid) repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.

U2 - 10.1126/science.aau3956

DO - 10.1126/science.aau3956

M3 - Article

VL - 362

SP - 1407

EP - 1410

JO - Science

JF - Science

SN - 0036-8075

IS - 6421

ER -

Orosa-Puente B, Leftley N, von Wangenheim D, Banda J, Srivastava AK, Hill K et al. Root branching toward water involves posttranslational modification of transcription factor ARF7. Science. 2018 Dec 21;362(6421):1407-1410. https://doi.org/10.1126/science.aau3956