Root branching toward water involves posttranslational modification of transcription factor ARF7

Beatriz Orosa-Puente, Nicola Leftley, Daniel von Wangenheim, Jason Banda, Anjil K. Srivastava, Kristine Hill, Jekaterina Truskina, Rahul Bhosale, Emily Morris, Moumita Srivastava, Britta Kümpers, Tatsuaki Goh, Hidehiro Fukaki, Joop E.M. Vermeer, Teva Vernoux, José R. Dinneny, Andrew P. French, Anthony Bishopp, Ari Sadanandom*, Malcolm J. Bennett

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

55 Citations (Scopus)

Abstract

Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water by using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells. This differential expression pattern is regulated by posttranslational modification of ARF7 with the small ubiquitin-like modifier (SUMO) protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA (indole-3-acetic acid) repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.

Original languageEnglish
Pages (from-to)1407-1410
JournalScience
Volume362
Issue number6421
DOIs
Publication statusPublished - 21 Dec 2018

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