Role of Tulipa gesneriana TEOSINTE BRANCHED1 (TgTB1) in the control of axillary bud outgrowth in bulbs

Natalia M. Moreno-Pachon, Marie Chantal Mutimawurugo, Eveline Heynen, Lidiya Sergeeva, Anne Benders, Ikram Blilou, Henk W.M. Hilhorst, Richard G.H. Immink*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Key message: Tulip vegetative reproduction. Abstract: Tulips reproduce asexually by the outgrowth of their axillary meristems located in the axil of each bulb scale. The number of axillary meristems in one bulb is low, and not all of them grow out during the yearly growth cycle of the bulb. Since the degree of axillary bud outgrowth in tulip determines the success of their vegetative propagation, this study aimed at understanding the mechanism controlling the differential axillary bud activity. We used a combined physiological and “bottom-up” molecular approach to shed light on this process and found that first two inner located buds do not seem to experience dormancy during the growth cycle, while mid-located buds enter dormancy by the end of the growing season. Dormancy was assessed by weight increase and TgTB1 expression levels, a conserved TCP transcription factor and well-known master integrator of environmental and endogenous signals influencing axillary meristem outgrowth in plants. We showed that TgTB1 expression in tulip bulbs can be modulated by sucrose, cytokinin and strigolactone, just as it has been reported for other species. However, the limited growth of mid-located buds, even when their TgTB1 expression is downregulated, points at other factors, probably physical, inhibiting their growth. We conclude that the time of axillary bud initiation determines the degree of dormancy and the sink strength of the bud. Thus, development, apical dominance, sink strength, hormonal cross-talk, expression of TgTB1 and other possibly physical but unidentified players, all converge to determine the growth capacity of tulip axillary buds.
Original languageEnglish
Pages (from-to)145-157
JournalPlant Reproduction
Volume31
Issue number2
Early online date7 Dec 2017
DOIs
Publication statusPublished - Jun 2018

Fingerprint

Tulipa
Tulipa gesneriana
bulbs
buds
dormancy
meristems
Meristem
Growth
bud initiation
apical dominance
vegetative propagation
Cytokinins
cytokinins
transcription factors
growing season
sucrose
Reproduction
Sucrose
Transcription Factors
Down-Regulation

Keywords

  • Apical dominance
  • Axillary bud
  • Dormancy
  • TgTB1

Cite this

Moreno-Pachon, Natalia M. ; Mutimawurugo, Marie Chantal ; Heynen, Eveline ; Sergeeva, Lidiya ; Benders, Anne ; Blilou, Ikram ; Hilhorst, Henk W.M. ; Immink, Richard G.H. / Role of Tulipa gesneriana TEOSINTE BRANCHED1 (TgTB1) in the control of axillary bud outgrowth in bulbs. In: Plant Reproduction. 2018 ; Vol. 31, No. 2. pp. 145-157.
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abstract = "Key message: Tulip vegetative reproduction. Abstract: Tulips reproduce asexually by the outgrowth of their axillary meristems located in the axil of each bulb scale. The number of axillary meristems in one bulb is low, and not all of them grow out during the yearly growth cycle of the bulb. Since the degree of axillary bud outgrowth in tulip determines the success of their vegetative propagation, this study aimed at understanding the mechanism controlling the differential axillary bud activity. We used a combined physiological and “bottom-up” molecular approach to shed light on this process and found that first two inner located buds do not seem to experience dormancy during the growth cycle, while mid-located buds enter dormancy by the end of the growing season. Dormancy was assessed by weight increase and TgTB1 expression levels, a conserved TCP transcription factor and well-known master integrator of environmental and endogenous signals influencing axillary meristem outgrowth in plants. We showed that TgTB1 expression in tulip bulbs can be modulated by sucrose, cytokinin and strigolactone, just as it has been reported for other species. However, the limited growth of mid-located buds, even when their TgTB1 expression is downregulated, points at other factors, probably physical, inhibiting their growth. We conclude that the time of axillary bud initiation determines the degree of dormancy and the sink strength of the bud. Thus, development, apical dominance, sink strength, hormonal cross-talk, expression of TgTB1 and other possibly physical but unidentified players, all converge to determine the growth capacity of tulip axillary buds.",
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Role of Tulipa gesneriana TEOSINTE BRANCHED1 (TgTB1) in the control of axillary bud outgrowth in bulbs. / Moreno-Pachon, Natalia M.; Mutimawurugo, Marie Chantal; Heynen, Eveline; Sergeeva, Lidiya; Benders, Anne; Blilou, Ikram; Hilhorst, Henk W.M.; Immink, Richard G.H.

In: Plant Reproduction, Vol. 31, No. 2, 06.2018, p. 145-157.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Role of Tulipa gesneriana TEOSINTE BRANCHED1 (TgTB1) in the control of axillary bud outgrowth in bulbs

AU - Moreno-Pachon, Natalia M.

AU - Mutimawurugo, Marie Chantal

AU - Heynen, Eveline

AU - Sergeeva, Lidiya

AU - Benders, Anne

AU - Blilou, Ikram

AU - Hilhorst, Henk W.M.

AU - Immink, Richard G.H.

PY - 2018/6

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N2 - Key message: Tulip vegetative reproduction. Abstract: Tulips reproduce asexually by the outgrowth of their axillary meristems located in the axil of each bulb scale. The number of axillary meristems in one bulb is low, and not all of them grow out during the yearly growth cycle of the bulb. Since the degree of axillary bud outgrowth in tulip determines the success of their vegetative propagation, this study aimed at understanding the mechanism controlling the differential axillary bud activity. We used a combined physiological and “bottom-up” molecular approach to shed light on this process and found that first two inner located buds do not seem to experience dormancy during the growth cycle, while mid-located buds enter dormancy by the end of the growing season. Dormancy was assessed by weight increase and TgTB1 expression levels, a conserved TCP transcription factor and well-known master integrator of environmental and endogenous signals influencing axillary meristem outgrowth in plants. We showed that TgTB1 expression in tulip bulbs can be modulated by sucrose, cytokinin and strigolactone, just as it has been reported for other species. However, the limited growth of mid-located buds, even when their TgTB1 expression is downregulated, points at other factors, probably physical, inhibiting their growth. We conclude that the time of axillary bud initiation determines the degree of dormancy and the sink strength of the bud. Thus, development, apical dominance, sink strength, hormonal cross-talk, expression of TgTB1 and other possibly physical but unidentified players, all converge to determine the growth capacity of tulip axillary buds.

AB - Key message: Tulip vegetative reproduction. Abstract: Tulips reproduce asexually by the outgrowth of their axillary meristems located in the axil of each bulb scale. The number of axillary meristems in one bulb is low, and not all of them grow out during the yearly growth cycle of the bulb. Since the degree of axillary bud outgrowth in tulip determines the success of their vegetative propagation, this study aimed at understanding the mechanism controlling the differential axillary bud activity. We used a combined physiological and “bottom-up” molecular approach to shed light on this process and found that first two inner located buds do not seem to experience dormancy during the growth cycle, while mid-located buds enter dormancy by the end of the growing season. Dormancy was assessed by weight increase and TgTB1 expression levels, a conserved TCP transcription factor and well-known master integrator of environmental and endogenous signals influencing axillary meristem outgrowth in plants. We showed that TgTB1 expression in tulip bulbs can be modulated by sucrose, cytokinin and strigolactone, just as it has been reported for other species. However, the limited growth of mid-located buds, even when their TgTB1 expression is downregulated, points at other factors, probably physical, inhibiting their growth. We conclude that the time of axillary bud initiation determines the degree of dormancy and the sink strength of the bud. Thus, development, apical dominance, sink strength, hormonal cross-talk, expression of TgTB1 and other possibly physical but unidentified players, all converge to determine the growth capacity of tulip axillary buds.

KW - Apical dominance

KW - Axillary bud

KW - Dormancy

KW - TgTB1

U2 - 10.1007/s00497-017-0316-z

DO - 10.1007/s00497-017-0316-z

M3 - Article

VL - 31

SP - 145

EP - 157

JO - Plant Reproduction

JF - Plant Reproduction

SN - 2194-7953

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