Role of the GacS sensor kinase in the regulation of volatile production by plant growth-promoting Pseudomonas fluorescens SBW25

Xu Cheng, Viviane Cordovez, Desalegn W. Etalo, M. van der Voort, Jos M. Raaijmakers

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

In plant-associated Pseudomonas species,the production of several secondary metabolites and exoenzymes is regulated by the GacS/GacA two-component regulatory system (the Gac-system). Here,we investigated if a mutation in the GacS sensor kinase affects the production of volatile organic compounds (VOCs) in P. fluorescens SBW25 (Pf.SBW25) and how this impacts on VOCs-mediated growth promotion and induced systemic resistance of Arabidopsis and tobacco. A total of 205 VOCs were detected by Gas Chromatography Mass Spectrometry for Pf. SBW25 and the gacS-mutant grown on two different media for 3 and 6 days. Discriminant function analysis followed by hierarchical clustering revealed 24 VOCs that were significantly different in their abundance between Pf.SBW25 and the gacS-mutant,which included three acyclic alkenes (3-nonene,4-undecyne,1-undecene). These alkenes were significantly reduced by the gacS mutation independently of the growth media and of the incubation time. For Arabidopsis,both Pf.SBW25 and the gacS-mutant enhanced,via VOCs,root and shoot biomass,induced systemic resistance against leaf infections by P. syringae and rhizosphere acidification to the same extent. For tobacco,however,VOCs-mediated effects on shoot and root growth were significantly different between Pf.SBW25 and the gacS-mutant. While Pf.SBW25 inhibited tobacco root growth,the gacS-mutant enhanced root biomass and lateral root formation relative to the non-treated control plants. Collectively these results indicate that the sensor kinase GacS is involved in the regulation of VOCs production in Pf.SBW25,affecting plant growth in a plant species-dependent manner.

LanguageEnglish
Article number1706
Number of pages10
JournalFrontiers in Plant Science
Volume7
DOIs
Publication statusPublished - 18 Nov 2016

Fingerprint

volatile organic compounds
Pseudomonas fluorescens
phosphotransferases (kinases)
plant growth
mutants
alkenes
tobacco
root growth
Arabidopsis
mutation
shoots
growth promotion
biomass
discriminant analysis
Pseudomonas
secondary metabolites
acidification
rhizosphere
culture media
infection

Keywords

  • GC-MS
  • ISR
  • Plant growth promotion
  • Pseudomonas
  • Two-component regulation
  • Volatile organic compounds

Cite this

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title = "Role of the GacS sensor kinase in the regulation of volatile production by plant growth-promoting Pseudomonas fluorescens SBW25",
abstract = "In plant-associated Pseudomonas species,the production of several secondary metabolites and exoenzymes is regulated by the GacS/GacA two-component regulatory system (the Gac-system). Here,we investigated if a mutation in the GacS sensor kinase affects the production of volatile organic compounds (VOCs) in P. fluorescens SBW25 (Pf.SBW25) and how this impacts on VOCs-mediated growth promotion and induced systemic resistance of Arabidopsis and tobacco. A total of 205 VOCs were detected by Gas Chromatography Mass Spectrometry for Pf. SBW25 and the gacS-mutant grown on two different media for 3 and 6 days. Discriminant function analysis followed by hierarchical clustering revealed 24 VOCs that were significantly different in their abundance between Pf.SBW25 and the gacS-mutant,which included three acyclic alkenes (3-nonene,4-undecyne,1-undecene). These alkenes were significantly reduced by the gacS mutation independently of the growth media and of the incubation time. For Arabidopsis,both Pf.SBW25 and the gacS-mutant enhanced,via VOCs,root and shoot biomass,induced systemic resistance against leaf infections by P. syringae and rhizosphere acidification to the same extent. For tobacco,however,VOCs-mediated effects on shoot and root growth were significantly different between Pf.SBW25 and the gacS-mutant. While Pf.SBW25 inhibited tobacco root growth,the gacS-mutant enhanced root biomass and lateral root formation relative to the non-treated control plants. Collectively these results indicate that the sensor kinase GacS is involved in the regulation of VOCs production in Pf.SBW25,affecting plant growth in a plant species-dependent manner.",
keywords = "GC-MS, ISR, Plant growth promotion, Pseudomonas, Two-component regulation, Volatile organic compounds",
author = "Xu Cheng and Viviane Cordovez and Etalo, {Desalegn W.} and {van der Voort}, M. and Raaijmakers, {Jos M.}",
year = "2016",
month = "11",
day = "18",
doi = "10.3389/fpls.2016.01706",
language = "English",
volume = "7",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
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}

Role of the GacS sensor kinase in the regulation of volatile production by plant growth-promoting Pseudomonas fluorescens SBW25. / Cheng, Xu; Cordovez, Viviane; Etalo, Desalegn W.; van der Voort, M.; Raaijmakers, Jos M.

In: Frontiers in Plant Science, Vol. 7, 1706, 18.11.2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Role of the GacS sensor kinase in the regulation of volatile production by plant growth-promoting Pseudomonas fluorescens SBW25

AU - Cheng, Xu

AU - Cordovez, Viviane

AU - Etalo, Desalegn W.

AU - van der Voort, M.

AU - Raaijmakers, Jos M.

PY - 2016/11/18

Y1 - 2016/11/18

N2 - In plant-associated Pseudomonas species,the production of several secondary metabolites and exoenzymes is regulated by the GacS/GacA two-component regulatory system (the Gac-system). Here,we investigated if a mutation in the GacS sensor kinase affects the production of volatile organic compounds (VOCs) in P. fluorescens SBW25 (Pf.SBW25) and how this impacts on VOCs-mediated growth promotion and induced systemic resistance of Arabidopsis and tobacco. A total of 205 VOCs were detected by Gas Chromatography Mass Spectrometry for Pf. SBW25 and the gacS-mutant grown on two different media for 3 and 6 days. Discriminant function analysis followed by hierarchical clustering revealed 24 VOCs that were significantly different in their abundance between Pf.SBW25 and the gacS-mutant,which included three acyclic alkenes (3-nonene,4-undecyne,1-undecene). These alkenes were significantly reduced by the gacS mutation independently of the growth media and of the incubation time. For Arabidopsis,both Pf.SBW25 and the gacS-mutant enhanced,via VOCs,root and shoot biomass,induced systemic resistance against leaf infections by P. syringae and rhizosphere acidification to the same extent. For tobacco,however,VOCs-mediated effects on shoot and root growth were significantly different between Pf.SBW25 and the gacS-mutant. While Pf.SBW25 inhibited tobacco root growth,the gacS-mutant enhanced root biomass and lateral root formation relative to the non-treated control plants. Collectively these results indicate that the sensor kinase GacS is involved in the regulation of VOCs production in Pf.SBW25,affecting plant growth in a plant species-dependent manner.

AB - In plant-associated Pseudomonas species,the production of several secondary metabolites and exoenzymes is regulated by the GacS/GacA two-component regulatory system (the Gac-system). Here,we investigated if a mutation in the GacS sensor kinase affects the production of volatile organic compounds (VOCs) in P. fluorescens SBW25 (Pf.SBW25) and how this impacts on VOCs-mediated growth promotion and induced systemic resistance of Arabidopsis and tobacco. A total of 205 VOCs were detected by Gas Chromatography Mass Spectrometry for Pf. SBW25 and the gacS-mutant grown on two different media for 3 and 6 days. Discriminant function analysis followed by hierarchical clustering revealed 24 VOCs that were significantly different in their abundance between Pf.SBW25 and the gacS-mutant,which included three acyclic alkenes (3-nonene,4-undecyne,1-undecene). These alkenes were significantly reduced by the gacS mutation independently of the growth media and of the incubation time. For Arabidopsis,both Pf.SBW25 and the gacS-mutant enhanced,via VOCs,root and shoot biomass,induced systemic resistance against leaf infections by P. syringae and rhizosphere acidification to the same extent. For tobacco,however,VOCs-mediated effects on shoot and root growth were significantly different between Pf.SBW25 and the gacS-mutant. While Pf.SBW25 inhibited tobacco root growth,the gacS-mutant enhanced root biomass and lateral root formation relative to the non-treated control plants. Collectively these results indicate that the sensor kinase GacS is involved in the regulation of VOCs production in Pf.SBW25,affecting plant growth in a plant species-dependent manner.

KW - GC-MS

KW - ISR

KW - Plant growth promotion

KW - Pseudomonas

KW - Two-component regulation

KW - Volatile organic compounds

U2 - 10.3389/fpls.2016.01706

DO - 10.3389/fpls.2016.01706

M3 - Article

VL - 7

JO - Frontiers in Plant Science

T2 - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 1706

ER -