Role of the GacS sensor kinase in the regulation of volatile production by plant growth-promoting Pseudomonas fluorescens SBW25

Xu Cheng, Viviane Cordovez, Desalegn W. Etalo, M. van der Voort, Jos M. Raaijmakers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

In plant-associated Pseudomonas species,the production of several secondary metabolites and exoenzymes is regulated by the GacS/GacA two-component regulatory system (the Gac-system). Here,we investigated if a mutation in the GacS sensor kinase affects the production of volatile organic compounds (VOCs) in P. fluorescens SBW25 (Pf.SBW25) and how this impacts on VOCs-mediated growth promotion and induced systemic resistance of Arabidopsis and tobacco. A total of 205 VOCs were detected by Gas Chromatography Mass Spectrometry for Pf. SBW25 and the gacS-mutant grown on two different media for 3 and 6 days. Discriminant function analysis followed by hierarchical clustering revealed 24 VOCs that were significantly different in their abundance between Pf.SBW25 and the gacS-mutant,which included three acyclic alkenes (3-nonene,4-undecyne,1-undecene). These alkenes were significantly reduced by the gacS mutation independently of the growth media and of the incubation time. For Arabidopsis,both Pf.SBW25 and the gacS-mutant enhanced,via VOCs,root and shoot biomass,induced systemic resistance against leaf infections by P. syringae and rhizosphere acidification to the same extent. For tobacco,however,VOCs-mediated effects on shoot and root growth were significantly different between Pf.SBW25 and the gacS-mutant. While Pf.SBW25 inhibited tobacco root growth,the gacS-mutant enhanced root biomass and lateral root formation relative to the non-treated control plants. Collectively these results indicate that the sensor kinase GacS is involved in the regulation of VOCs production in Pf.SBW25,affecting plant growth in a plant species-dependent manner.

Original languageEnglish
Article number1706
Number of pages10
JournalFrontiers in Plant Science
Volume7
DOIs
Publication statusPublished - 18 Nov 2016

Keywords

  • GC-MS
  • ISR
  • Plant growth promotion
  • Pseudomonas
  • Two-component regulation
  • Volatile organic compounds

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