Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1

Mónica D. Fernández Ramírez, Masja N. Nierop Groot, Eddy J. Smid, Pascal Hols, Michiel Kleerebezem, Tjakko Abee*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products. This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1–4, Δcps1–4). Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain. Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity. Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.
Original languageEnglish
Pages (from-to)15-23
JournalInternational Journal of Food Microbiology
Volume271
DOIs
Publication statusPublished - 20 Apr 2018

Fingerprint

Lactobacillus plantarum
Biofilms
biofilm
mutants
Gentian Violet
gentian violet
cells
sortase A
Staining and Labeling
Autolysis
Food
food spoilage
Food Handling
autolysis
Peptidoglycan
DNA
peptidoglycans
Polystyrenes
polystyrenes
surface proteins

Keywords

  • Autolysis
  • Capsular polysaccharides
  • Extracellular DNA
  • Sortase A

Cite this

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title = "Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1",
abstract = "Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products. This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1–4, Δcps1–4). Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain. Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity. Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.",
keywords = "Autolysis, Capsular polysaccharides, Extracellular DNA, Sortase A",
author = "{Fern{\'a}ndez Ram{\'i}rez}, {M{\'o}nica D.} and {Nierop Groot}, {Masja N.} and Smid, {Eddy J.} and Pascal Hols and Michiel Kleerebezem and Tjakko Abee",
year = "2018",
month = "4",
day = "20",
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language = "English",
volume = "271",
pages = "15--23",
journal = "International Journal of Food Microbiology",
issn = "0168-1605",
publisher = "Elsevier",

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Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1. / Fernández Ramírez, Mónica D.; Nierop Groot, Masja N.; Smid, Eddy J.; Hols, Pascal; Kleerebezem, Michiel; Abee, Tjakko.

In: International Journal of Food Microbiology, Vol. 271, 20.04.2018, p. 15-23.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Role of cell surface composition and lysis in static biofilm formation by Lactobacillus plantarum WCFS1

AU - Fernández Ramírez, Mónica D.

AU - Nierop Groot, Masja N.

AU - Smid, Eddy J.

AU - Hols, Pascal

AU - Kleerebezem, Michiel

AU - Abee, Tjakko

PY - 2018/4/20

Y1 - 2018/4/20

N2 - Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products. This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1–4, Δcps1–4). Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain. Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity. Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.

AB - Next to applications in fermentations, Lactobacillus plantarum is recognized as a food spoilage organism, and its dispersal from biofilms in food processing environments might be implicated in contamination or recontamination of food products. This study provides new insights into biofilm development by L. plantarum WCFS1 through comparative analysis of wild type and mutants affected in cell surface composition, including mutants deficient in the production of Sortase A involved in the covalent attachment of 27 predicted surface proteins to the cell wall peptidoglycan (ΔsrtA) and mutants deficient in the production of capsular polysaccharides (CPS1–4, Δcps1–4). Surface adhesion and biofilm formation studies revealed none of the imposed cell surface modifications to affect the initial attachment of cells to polystyrene while biofilm formation based on Crystal Violet (CV) staining was severely reduced in the ΔsrtA mutant and significantly increased in mutants lacking the cps1 cluster, compared to the wild-type strain. Fluorescence microscopy analysis of biofilm samples pointed to a higher presence of extracellular DNA (eDNA) in cps1 mutants and this corresponded with increased autolysis activity. Subsequent studies using Δacm2 and ΔlytA derivatives affected in lytic behaviour revealed reduced biofilm formation measured by CV staining, confirming the relevance of lysis for the build-up of the biofilm matrix with eDNA.

KW - Autolysis

KW - Capsular polysaccharides

KW - Extracellular DNA

KW - Sortase A

U2 - 10.1016/j.ijfoodmicro.2018.02.013

DO - 10.1016/j.ijfoodmicro.2018.02.013

M3 - Article

VL - 271

SP - 15

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JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

ER -