Robust sampling and preservation of DNA for microbial community profiling in field experiments

Anneloes E. Groenenboom*, Eddy J. Smid, Sijmen E. Schoustra

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Objective: Stabilising samples of microbial communities for DNA extraction without access to laboratory equipment can be a challenging task. In this paper we propose a method using filter paper disks for the preservation of DNA from diverse microbial communities which are found in a fermented milk product. Results: Small adaptations to the DNA extraction method used for liquid fermented milk delivered DNA of sufficient amounts and quality to be used for later analyses, e.g. full community 16S amplicon sequencing. The microbial community structure obtained via the filter paper method showed sufficient resemblance to the structure obtain via the traditional DNA extraction from the liquid milk sample. This method can therefore successfully be used to analyse diverse microbial communities from fermented milk products from remote areas.

Original languageEnglish
Article number159
JournalBMC Research Notes
Volume12
Issue number1
DOIs
Publication statusPublished - 22 Mar 2019

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Keywords

  • DNA stabilisation
  • Fermentation
  • Field trial
  • Filter paper disks
  • Microbial community
  • Milk

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