RNA interference silencing of chalcone synthase, the first step in the flavonoid biosynthesis pathway, leads to parthenocarpic tomato fruits

E.G.W.M. Schijlen, C.H. de Vos, S. Martens, H.H. Jonker, F.M.A. Rosin, J.W. Molthoff, Y.M. Tikunov, G.C. Angenent, A.J. van Tunen, A.G. Bovy

    Research output: Contribution to journalArticleAcademicpeer-review

    154 Citations (Scopus)

    Abstract

    Parthenocarpy, the formation of seedless fruits in the absence of functional fertilization, is a desirable trait for several important crop plants, including tomato (Solanum lycopersicum). Seedless fruits can be of great value for consumers, the processing industry, and breeding companies. In this article, we propose a novel strategy to obtain parthenocarpic tomatoes by down-regulation of the flavonoid biosynthesis pathway using RNA interference (RNAi)-mediated suppression of chalcone synthase (CHS), the first gene in the flavonoid pathway. In CHS RNAi plants, total flavonoid levels, transcript levels of both Chs1 and Chs2, as well as CHS enzyme activity were reduced by up to a few percent of the corresponding wild-type values. Surprisingly, all strong Chs-silenced tomato lines developed parthenocarpic fruits. Although a relation between flavonoids and parthenocarpic fruit development has never been described, it is well known that flavonoids are essential for pollen development and pollen tube growth and, hence, play an essential role in plant reproduction. The observed parthenocarpic fruit development appeared to be pollination dependent, and Chs RNAi fruits displayed impaired pollen tube growth. Our results lead to novel insight in the mechanisms underlying parthenocarpic fruit development. The potential of this technology for applications in plant breeding and biotechnology will be discussed.
    Original languageEnglish
    Pages (from-to)1520-1530
    JournalPlant Physiology
    Volume144
    Issue number3
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    naringenin-chalcone synthase
    Lycopersicon esculentum
    RNA Interference
    RNA interference
    Flavonoids
    Fruit
    flavonoids
    tomatoes
    biosynthesis
    fruits
    fruiting
    pollen tubes
    Pollen Tube
    parthenocarpy
    plant reproduction
    application technology
    Solanum lycopersicum
    fertilization (reproduction)
    plant breeding
    biotechnology

    Keywords

    • transcription factor
    • male-sterility
    • arabidopsis-thaliana
    • male-fertility
    • flower color
    • tube growth
    • gene
    • pollen
    • petunia
    • expression

    Cite this

    @article{68ad7131bcf24ce5b61205204b815526,
    title = "RNA interference silencing of chalcone synthase, the first step in the flavonoid biosynthesis pathway, leads to parthenocarpic tomato fruits",
    abstract = "Parthenocarpy, the formation of seedless fruits in the absence of functional fertilization, is a desirable trait for several important crop plants, including tomato (Solanum lycopersicum). Seedless fruits can be of great value for consumers, the processing industry, and breeding companies. In this article, we propose a novel strategy to obtain parthenocarpic tomatoes by down-regulation of the flavonoid biosynthesis pathway using RNA interference (RNAi)-mediated suppression of chalcone synthase (CHS), the first gene in the flavonoid pathway. In CHS RNAi plants, total flavonoid levels, transcript levels of both Chs1 and Chs2, as well as CHS enzyme activity were reduced by up to a few percent of the corresponding wild-type values. Surprisingly, all strong Chs-silenced tomato lines developed parthenocarpic fruits. Although a relation between flavonoids and parthenocarpic fruit development has never been described, it is well known that flavonoids are essential for pollen development and pollen tube growth and, hence, play an essential role in plant reproduction. The observed parthenocarpic fruit development appeared to be pollination dependent, and Chs RNAi fruits displayed impaired pollen tube growth. Our results lead to novel insight in the mechanisms underlying parthenocarpic fruit development. The potential of this technology for applications in plant breeding and biotechnology will be discussed.",
    keywords = "transcription factor, male-sterility, arabidopsis-thaliana, male-fertility, flower color, tube growth, gene, pollen, petunia, expression",
    author = "E.G.W.M. Schijlen and {de Vos}, C.H. and S. Martens and H.H. Jonker and F.M.A. Rosin and J.W. Molthoff and Y.M. Tikunov and G.C. Angenent and {van Tunen}, A.J. and A.G. Bovy",
    year = "2007",
    doi = "10.1104/pp.107.100305",
    language = "English",
    volume = "144",
    pages = "1520--1530",
    journal = "Plant Physiology",
    issn = "0032-0889",
    publisher = "American Society of Plant Biologists",
    number = "3",

    }

    RNA interference silencing of chalcone synthase, the first step in the flavonoid biosynthesis pathway, leads to parthenocarpic tomato fruits. / Schijlen, E.G.W.M.; de Vos, C.H.; Martens, S.; Jonker, H.H.; Rosin, F.M.A.; Molthoff, J.W.; Tikunov, Y.M.; Angenent, G.C.; van Tunen, A.J.; Bovy, A.G.

    In: Plant Physiology, Vol. 144, No. 3, 2007, p. 1520-1530.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - RNA interference silencing of chalcone synthase, the first step in the flavonoid biosynthesis pathway, leads to parthenocarpic tomato fruits

    AU - Schijlen, E.G.W.M.

    AU - de Vos, C.H.

    AU - Martens, S.

    AU - Jonker, H.H.

    AU - Rosin, F.M.A.

    AU - Molthoff, J.W.

    AU - Tikunov, Y.M.

    AU - Angenent, G.C.

    AU - van Tunen, A.J.

    AU - Bovy, A.G.

    PY - 2007

    Y1 - 2007

    N2 - Parthenocarpy, the formation of seedless fruits in the absence of functional fertilization, is a desirable trait for several important crop plants, including tomato (Solanum lycopersicum). Seedless fruits can be of great value for consumers, the processing industry, and breeding companies. In this article, we propose a novel strategy to obtain parthenocarpic tomatoes by down-regulation of the flavonoid biosynthesis pathway using RNA interference (RNAi)-mediated suppression of chalcone synthase (CHS), the first gene in the flavonoid pathway. In CHS RNAi plants, total flavonoid levels, transcript levels of both Chs1 and Chs2, as well as CHS enzyme activity were reduced by up to a few percent of the corresponding wild-type values. Surprisingly, all strong Chs-silenced tomato lines developed parthenocarpic fruits. Although a relation between flavonoids and parthenocarpic fruit development has never been described, it is well known that flavonoids are essential for pollen development and pollen tube growth and, hence, play an essential role in plant reproduction. The observed parthenocarpic fruit development appeared to be pollination dependent, and Chs RNAi fruits displayed impaired pollen tube growth. Our results lead to novel insight in the mechanisms underlying parthenocarpic fruit development. The potential of this technology for applications in plant breeding and biotechnology will be discussed.

    AB - Parthenocarpy, the formation of seedless fruits in the absence of functional fertilization, is a desirable trait for several important crop plants, including tomato (Solanum lycopersicum). Seedless fruits can be of great value for consumers, the processing industry, and breeding companies. In this article, we propose a novel strategy to obtain parthenocarpic tomatoes by down-regulation of the flavonoid biosynthesis pathway using RNA interference (RNAi)-mediated suppression of chalcone synthase (CHS), the first gene in the flavonoid pathway. In CHS RNAi plants, total flavonoid levels, transcript levels of both Chs1 and Chs2, as well as CHS enzyme activity were reduced by up to a few percent of the corresponding wild-type values. Surprisingly, all strong Chs-silenced tomato lines developed parthenocarpic fruits. Although a relation between flavonoids and parthenocarpic fruit development has never been described, it is well known that flavonoids are essential for pollen development and pollen tube growth and, hence, play an essential role in plant reproduction. The observed parthenocarpic fruit development appeared to be pollination dependent, and Chs RNAi fruits displayed impaired pollen tube growth. Our results lead to novel insight in the mechanisms underlying parthenocarpic fruit development. The potential of this technology for applications in plant breeding and biotechnology will be discussed.

    KW - transcription factor

    KW - male-sterility

    KW - arabidopsis-thaliana

    KW - male-fertility

    KW - flower color

    KW - tube growth

    KW - gene

    KW - pollen

    KW - petunia

    KW - expression

    U2 - 10.1104/pp.107.100305

    DO - 10.1104/pp.107.100305

    M3 - Article

    VL - 144

    SP - 1520

    EP - 1530

    JO - Plant Physiology

    JF - Plant Physiology

    SN - 0032-0889

    IS - 3

    ER -