RNA interference in Lepidoptera: An overview of successful and unsuccessful

O. Terenius, A. Papanicolaou, J.S. Garbutt, I. Eleftherianos, H. Huvenne, S. Kanginakudru, M. Albrechtsen, Chunju An, J.L. Aymeric, A. Barthel, P. Bebas, K. Bitra, A. Bravo, F. Chevalier, D.P. Collinge, C.M. Crava, R.A. de Maagd, B. Duvic, M. Erlandson, I. FayeG. Felfoldi, H. Fujiwara, R. Futahashi, A.S. Gandhe, H.S. Gatehouse, L.N. Gatehouse, J.M. Giebultowicz, I. Gomez, C.J.P. Grimmelikhuijzen, A.T. Groot, F. Hauser, D.G. Heckel, D.D. Hegedus, S. Hrycaj, L. Huang, J.J. Hull, K. Iatrou, M. Iga, M.R. Kanost, J. Kotwica, Changyou Li, Jianghong Li, Jisheng Liu, M. Lundmark, S. Matsumoto, M. Meyering-Vos, P.J. Millichap, A. Monteiro, N. Mrinal, T. Niimi, D. Nowara, A. Ohnishi, V. Oostra, K. Ozaki, M. Papakonstantinou, A. Popadic, M.V. Rajam, S. Saenko, R.M. Simpson, M. Soberon, M.R. Strand, S. Tomita, U. Toprak, Ping Wang, Choon Wei Wee, S. Whyard, Wenqing Zhang, J. Nagaraju, R.H. Ffrench-Constant, S. Herrero, K. Gordon, L. Swevers, G. Smagghe

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Abstract

Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments
Original languageEnglish
Pages (from-to)231-245
JournalJournal of Insect Physiology
Volume57
Issue number2
DOIs
Publication statusPublished - 2011

Keywords

  • double-stranded-rna
  • armyworm spodoptera-frugiperda
  • silkworm bombyx-mori
  • small silencing rnas
  • manduca-sexta
  • bacillus-thuringiensis
  • caenorhabditis-elegans
  • helicoverpa-armigera
  • immune-responses
  • messenger-rna

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