RLP/K enrichment sequencing; a novel method to identify receptor-like protein (RLP) and receptor-like kinase (RLK) genes

Xiao Lin, Miles Armstrong, Katie Baker, Doret Wouters, Richard G.F. Visser, Pieter J. Wolters, Ingo Hein*, Vivianne G.A.A. Vleeshouwers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

The identification of immune receptors in crop plants is time-consuming but important for disease control. Previously, resistance gene enrichment sequencing (RenSeq) was developed to accelerate mapping of nucleotide-binding domain and leucine-rich repeat containing (NLR) genes. However, resistances mediated by pattern recognition receptors (PRRs) remain less utilized. Here, our pipeline shows accelerated mapping of PRRs. Effectoromics leads to precise identification of plants with target PRRs, and subsequent RLP/K enrichment sequencing (RLP/KSeq) leads to detection of informative single nucleotide polymorphisms that are linked to the trait. Using Phytophthora infestans as a model, we identified Solanum microdontum plants that recognize the apoplastic effectors INF1 or SCR74. RLP/KSeq in a segregating Solanum population confirmed the localization of the INF1 receptor on chromosome 12, and led to the rapid mapping of the response to SCR74 to chromosome 9. By using markers obtained from RLP/KSeq in conjunction with additional markers, we fine-mapped the SCR74 receptor to a 43-kbp G-LecRK locus. Our findings show that RLP/KSeq enables rapid mapping of PRRs and is especially beneficial for crop plants with large and complex genomes. This work will enable the elucidation and characterization of the nonNLR plant immune receptors and ultimately facilitate informed resistance breeding.

Original languageEnglish
Pages (from-to)1264-1276
JournalNew Phytologist
Volume227
Issue number4
Early online date14 Apr 2020
DOIs
Publication statusPublished - Aug 2020

Keywords

  • genotyping by sequencing (GBS)
  • pattern recognition receptor (PRR)
  • Phytophthora infestans
  • potato
  • receptor-like kinase (RLK)
  • receptor-like protein (RLP)
  • RenSeq
  • RLP/K enrichment sequencing (RLP/KSeq)

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