Abstract
| Original language | English |
|---|---|
| Patent number | WO2016166310 |
| Priority date | 16/04/15 |
| Publication status | Published - 20 Oct 2016 |
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Riboswitch inducible gene expression. / Creutzburg, S.C.A. (Inventor); van der Oost, J. (Inventor).
Patent No.: WO2016166310.Research output: Patent › Other research output
TY - PAT
T1 - Riboswitch inducible gene expression
AU - Creutzburg, S.C.A.
AU - van der Oost, J.
PY - 2016/10/20
Y1 - 2016/10/20
N2 - An intronic, self-splicing riboswitch is configured for enzyme-product specificity by introducing an appropriate aptamer. This then provides a sensing-expression construct, whereby the presence of an enzyme product in the cell triggers self-splicing of the intron sequence to restore the reading frame of the reporter gene and as such to drive expression of the gene product. The sensing construct expresses a protein which marks the cell or permits its growth or survival in or on an otherwise selective media. In this way, introduction or the presence of such product sensing-reporter constructs in cells can be harnessed to provide a multi-parallel rapid screening of cells or libraries for desirable enzyme variants.
AB - An intronic, self-splicing riboswitch is configured for enzyme-product specificity by introducing an appropriate aptamer. This then provides a sensing-expression construct, whereby the presence of an enzyme product in the cell triggers self-splicing of the intron sequence to restore the reading frame of the reporter gene and as such to drive expression of the gene product. The sensing construct expresses a protein which marks the cell or permits its growth or survival in or on an otherwise selective media. In this way, introduction or the presence of such product sensing-reporter constructs in cells can be harnessed to provide a multi-parallel rapid screening of cells or libraries for desirable enzyme variants.
M3 - Patent
M1 - WO2016166310
ER -