Riboswitch inducible gene expression

S.C.A. Creutzburg (Inventor), J. van der Oost (Inventor)

Research output: PatentOther research output

Abstract

An intronic, self-splicing riboswitch is configured for enzyme-product specificity by introducing an appropriate aptamer. This then provides a sensing-expression construct, whereby the presence of an enzyme product in the cell triggers self-splicing of the intron sequence to restore the reading frame of the reporter gene and as such to drive expression of the gene product. The sensing construct expresses a protein which marks the cell or permits its growth or survival in or on an otherwise selective media. In this way, introduction or the presence of such product sensing-reporter constructs in cells can be harnessed to provide a multi-parallel rapid screening of cells or libraries for desirable enzyme variants.
Original languageEnglish
Patent numberWO2016166310
Priority date16/04/15
Publication statusPublished - 20 Oct 2016

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