Rhizobium-legume symbiosis shares an exocytotic pathway required for arbuscle formation

S. Ivanov, E.E. Fedorova, E.H.M. Limpens, S. De Mita, A. Genre, P. Bonfante, T. Bisseling

Research output: Contribution to journalArticleAcademicpeer-review

123 Citations (Scopus)

Abstract

Endosymbiotic interactions are characterized by the formation of specialized membrane compartments, by the host in which the microbes are hosted, in an intracellular manner. Two well-studied examples, which are of major agricultural and ecological importance, are the widespread arbuscular mycorrhizal symbiosis and the Rhizobium–legume symbiosis. In both symbioses, the specialized host membrane that surrounds the microbes forms a symbiotic interface, which facilitates the exchange of, for example, nutrients in a controlled manner and, therefore, forms the heart of endosymbiosis. Despite their key importance, the molecular and cellular mechanisms underlying the formation of these membrane interfaces are largely unknown. Recent studies strongly suggest that the Rhizobium–legume symbiosis coopted a signaling pathway, including receptor, from the more ancient arbuscular mycorrhizal symbiosis to form a symbiotic interface. Here, we show that two highly homologous exocytotic vesicle-associated membrane proteins (VAMPs) are required for formation of the symbiotic membrane interface in both interactions. Silencing of these Medicago VAMP72 genes has a minor effect on nonsymbiotic plant development and nodule formation. However, it blocks symbiosome as well as arbuscule formation, whereas root colonization by the microbes is not affected. Identification of these VAMP72s as common symbiotic regulators in exocytotic vesicle trafficking suggests that the ancient exocytotic pathway forming the periarbuscular membrane compartment has also been coopted in the Rhizobium–legume symbiosis.
Original languageEnglish
Pages (from-to)8316-8321
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number21
DOIs
Publication statusPublished - 2012

Keywords

  • medicago-truncatula
  • root-nodules
  • intracellular accommodation
  • n-2-fixing symbiosomes
  • mycorrhizal symbiosis
  • infection threads
  • cell wall
  • membrane
  • genes
  • nodulation

Fingerprint Dive into the research topics of 'Rhizobium-legume symbiosis shares an exocytotic pathway required for arbuscle formation'. Together they form a unique fingerprint.

Cite this