RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls

Michiel T. Klaassen, Luisa M. Trindade*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Potato cell walls (PCW) are a low value by-product from the potato starch industry. Valorisation of PCW is hindered by its high water-binding capacity (WBC). The composition of polysaccharides and interactions between these entities, play important roles in regulating the WBC in the cell wall matrix. Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6–9%. In contrast, exo-truncation of these side-chains increased the WBC by 13% in vitro. We propose that degradation of RG-I galactan side-chains altered the WBC of PCW, due to cell wall remodelling and loosening that affected the porosity. Our findings reinforce the view that RG-I galactan side-chains play a role in modulating WBC, presumably by affecting polysaccharide architecture (spacing) and interactions in the matrix. Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.

Original languageEnglish
Article number115353
JournalCarbohydrate Polymers
Volume227
DOIs
Publication statusPublished - 1 Jan 2020

Fingerprint

Galactans
Cells
Water
Polysaccharides
Byproducts
Starch
Macromolecules
Porosity
Degradation
Chemical analysis

Keywords

  • Pectin
  • Potato cell walls (PCW)
  • Rhamnogalacturonan I (RG-I)β-(1→4)-D-galactan
  • Rhamnogalacturonan I, β-(1→4)-D-galactan
  • Water-binding capacity (WBC)
  • β-galactosidase

Cite this

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title = "RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls",
abstract = "Potato cell walls (PCW) are a low value by-product from the potato starch industry. Valorisation of PCW is hindered by its high water-binding capacity (WBC). The composition of polysaccharides and interactions between these entities, play important roles in regulating the WBC in the cell wall matrix. Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6–9{\%}. In contrast, exo-truncation of these side-chains increased the WBC by 13{\%} in vitro. We propose that degradation of RG-I galactan side-chains altered the WBC of PCW, due to cell wall remodelling and loosening that affected the porosity. Our findings reinforce the view that RG-I galactan side-chains play a role in modulating WBC, presumably by affecting polysaccharide architecture (spacing) and interactions in the matrix. Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.",
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author = "Klaassen, {Michiel T.} and Trindade, {Luisa M.}",
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RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls. / Klaassen, Michiel T.; Trindade, Luisa M.

In: Carbohydrate Polymers, Vol. 227, 115353, 01.01.2020.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - RG-I galactan side-chains are involved in the regulation of the water-binding capacity of potato cell walls

AU - Klaassen, Michiel T.

AU - Trindade, Luisa M.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Potato cell walls (PCW) are a low value by-product from the potato starch industry. Valorisation of PCW is hindered by its high water-binding capacity (WBC). The composition of polysaccharides and interactions between these entities, play important roles in regulating the WBC in the cell wall matrix. Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6–9%. In contrast, exo-truncation of these side-chains increased the WBC by 13% in vitro. We propose that degradation of RG-I galactan side-chains altered the WBC of PCW, due to cell wall remodelling and loosening that affected the porosity. Our findings reinforce the view that RG-I galactan side-chains play a role in modulating WBC, presumably by affecting polysaccharide architecture (spacing) and interactions in the matrix. Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.

AB - Potato cell walls (PCW) are a low value by-product from the potato starch industry. Valorisation of PCW is hindered by its high water-binding capacity (WBC). The composition of polysaccharides and interactions between these entities, play important roles in regulating the WBC in the cell wall matrix. Here, we show that in vivo exo-truncation of RG-I β-(1→4)-D-galactan side-chains decreased the WBC by 6–9%. In contrast, exo-truncation of these side-chains increased the WBC by 13% in vitro. We propose that degradation of RG-I galactan side-chains altered the WBC of PCW, due to cell wall remodelling and loosening that affected the porosity. Our findings reinforce the view that RG-I galactan side-chains play a role in modulating WBC, presumably by affecting polysaccharide architecture (spacing) and interactions in the matrix. Better understanding of structure-function relationships of pectin macromolecules is needed before cell wall by-products may be tailored to render added-value in food and biobased products.

KW - Pectin

KW - Potato cell walls (PCW)

KW - Rhamnogalacturonan I (RG-I)β-(1→4)-D-galactan

KW - Rhamnogalacturonan I, β-(1→4)-D-galactan

KW - Water-binding capacity (WBC)

KW - β-galactosidase

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M3 - Article

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JO - Carbohydrate Polymers

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