(R)evolution-on-a-chip

Evgenios Bouzetos; Ketan Ashok Ganar; Enrico Mastrobattista; Siddharth Deshpande; John van der Oost

doi:10.1016/j.tibtech.2021.04.009

(R)evolution-on-a-chip

Evgenios Bouzetos
, Ketan Ashok Ganar
, Enrico Mastrobattista
, Siddharth Deshpande^*
, John van der Oost^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

24 Citations (Scopus)

Abstract

High-throughput droplet generation using on-chip microfluidics allows for screening genetic expression libraries in monodisperse compartments in which a genotype to phenotype linkage is maintained.Synthetic compartments are compatible with in vitro expression of genes, making them ideal containers for laboratory evolution.Fluorescence-independent screening methods increase the scope of microfluidics-assisted in vitro compartmentalization (μΙVC).μΙVC is progressing rapidly from pilot stage to industrial applications.

Original language	English
Pages (from-to)	60-76
Number of pages	17
Journal	Trends in Biotechnology
Volume	40
Issue number	1
Early online date	25 May 2021
DOIs	https://doi.org/10.1016/j.tibtech.2021.04.009
Publication status	Published - Jan 2022

Keywords

biocatalyst
emulsion
in vitro compartmentalization
laboratory evolution
microfluidics
vesicle

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10.1016/j.tibtech.2021.04.009Licence: CC BY

https://edepot.wur.nl/549732Licence: CC BY

Cite this

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abstract = "High-throughput droplet generation using on-chip microfluidics allows for screening genetic expression libraries in monodisperse compartments in which a genotype to phenotype linkage is maintained.Synthetic compartments are compatible with in vitro expression of genes, making them ideal containers for laboratory evolution.Fluorescence-independent screening methods increase the scope of microfluidics-assisted in vitro compartmentalization (μΙVC).μΙVC is progressing rapidly from pilot stage to industrial applications.",

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AU - Mastrobattista, Enrico

AU - Deshpande, Siddharth

AU - van der Oost, John

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KW - biocatalyst

KW - emulsion

KW - in vitro compartmentalization

KW - laboratory evolution

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DO - 10.1016/j.tibtech.2021.04.009

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JO - Trends in Biotechnology

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(R)evolution-on-a-chip

Abstract

Keywords

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