(R)evolution-on-a-chip

Evgenios Bouzetos, Ketan Ashok Ganar, Enrico Mastrobattista, Siddharth Deshpande*, John van der Oost*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

High-throughput droplet generation using on-chip microfluidics allows for screening genetic expression libraries in monodisperse compartments in which a genotype to phenotype linkage is maintained.Synthetic compartments are compatible with in vitro expression of genes, making them ideal containers for laboratory evolution.Fluorescence-independent screening methods increase the scope of microfluidics-assisted in vitro compartmentalization (μΙVC).μΙVC is progressing rapidly from pilot stage to industrial applications.
Original languageEnglish
Pages (from-to)60-76
Number of pages17
JournalTrends in Biotechnology
Volume40
Issue number1
Early online date25 May 2021
DOIs
Publication statusPublished - 2022

Keywords

  • biocatalyst
  • emulsion
  • in vitro compartmentalization
  • laboratory evolution
  • microfluidics
  • vesicle

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