Abstract
High-throughput droplet generation using on-chip microfluidics allows for screening genetic expression libraries in monodisperse compartments in which a genotype to phenotype linkage is maintained.Synthetic compartments are compatible with in vitro expression of genes, making them ideal containers for laboratory evolution.Fluorescence-independent screening methods increase the scope of microfluidics-assisted in vitro compartmentalization (μΙVC).μΙVC is progressing rapidly from pilot stage to industrial applications.
Original language | English |
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Pages (from-to) | 60-76 |
Number of pages | 17 |
Journal | Trends in Biotechnology |
Volume | 40 |
Issue number | 1 |
Early online date | 25 May 2021 |
DOIs | |
Publication status | Published - Jan 2022 |
Keywords
- biocatalyst
- emulsion
- in vitro compartmentalization
- laboratory evolution
- microfluidics
- vesicle