Revealing methyl-esterification patterns of pectins by enzymatic fingerprinting: Beyond the degree of blockiness

Éva Jermendi, Martin Beukema, Marco A. van den Berg, Paul de Vos, Henk A. Schols*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Citrus pectins were studied by enzymatic fingerprinting using a simultaneous enzyme treatment with endo-polygalacturonase (endo-PG) from Kluyveromyces fragilis and pectin lyase (PL) from Aspergillus niger to reveal the methyl-ester distribution patterns over the pectin backbone. Using HILIC-MS combined with HPAEC enabled the separation and identification of the diagnostic oligomers released. Structural information on the pectins was provided by using novel descriptive parameters such as degree of blockiness of methyl-esterified oligomers by PG (DBPGme) and degree of blockiness of methyl-esterified oligomers by PL (DBPLme). This approach enabled us to clearly differentiate citrus pectins with various methyl-esterification patterns. The simultaneous use of PG and PL showed additional information, which is not revealed in digests using PG or PL alone. This approach can be valuable to differentiate pectins having the same DM and to get specific structural information on pectins and therefore to be able to better predict their physical and biochemical functionalities.

Original languageEnglish
Article number118813
JournalCarbohydrate Polymers
Volume277
DOIs
Publication statusPublished - 1 Feb 2022

Keywords

  • Citrus pectin
  • Degree of blockiness
  • Endo-polygalacturonase
  • HILIC-MS
  • HPAEC
  • Pectin lyase

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