Resistance breeding using molecular biological techniques

E. Jacobsen*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

    Abstract

    The breeder's tool-box contains molecular biological techniques to improve resistance breeding. In this chapter, the development of genetic maps is discussed in self-fertilisers and cross-fertilisers with some potential applications like indirect 'marker dependent' selection for qualitative and quantitative resistance traits and their limitations. The bulked segregant analysis has been mentioned for the simplified detection of linkage between resistances and RFLP markers. The possibilities and limitations of map-based cloning and cloning by insertion mutagenesis with TDNA and transposons are described. The role of Arabidopsis thaliana for the development of these rather complicated cloning strategies is shown. The potential use of cloned endogenous resistance genes in breeding is briefly discussed.

    Original languageEnglish
    Title of host publicationModern crop protection
    Subtitle of host publicationdevelopments and perspectives
    EditorsJ.C. Zadoks
    Place of PublicationWageningen
    PublisherBrill Nijhoff
    Pages83-90
    ISBN (Electronic)9789004684485
    ISBN (Print)9789074134064
    DOIs
    Publication statusPublished - 1993

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