Replication of white spot syndrome virus (WSSV) in the polychaete Dendronereis spp

H. Desrina, J.A.J. Verreth, S.B. Prayitno, J.H.W.M. Rombout, J.M. Vlak, M.C.J. Verdegem

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27 Citations (Scopus)

Abstract

This study investigated whether WSSV replicates in naturally infected Dendronereis spp., a common polychaete (Nereididae) species in shrimp ponds in Indonesia. To detect WSSV replication, (i) immunohistochemistry (IHC) using a monoclonal antibody against WSSV VP28 protein and (ii) nested RT-PCR using specific primers set for the vp28 gene to detect WSSV-specific mRNA were applied. WSSV immunoreactive-nuclei were detected in the gut epithelium of the polychaete and WSSV mRNA was detected with nested RT-PCR. This, together with the IHC results, confirmed that WSSV could replicate in Dendronereis spp. This is the first report showing that WSSV replicated in a naturally infected non-crustacean host.
Original languageEnglish
Pages (from-to)7-10
JournalJournal of Invertebrate Pathology
Volume114
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • morphogenesis
  • community
  • thailand
  • water
  • ponds
  • dna

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