Replication-Deficient Particles: New Insights into the Next Generation of Bleutongue Virus Vaccines

Cristina C. Celma, Meredith Stewart, Kerstine Wernike, Michael Eschbaumer, Lorenzo Gonzalez-Molleda, Emmanuel Breard, Claudia Schulz, Bernd Hoffmann, Andy Haegeman, Kris De Clercq, P.A. van Rijn

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10 Citations (Scopus)

Abstract

Bluetongue virus (BTV) is endemic in many parts of the world, often causing severe haemorrhagic disease in livestock. To date, at least 27 different serotypes have been recognized. Vaccination against all serotypes is necessary to protect susceptible animals and to prevent onward spread of the virus by insect vectors. In our previous studies, we generated replication-deficient (DISC) virus strains for a number of serotypes and reported preliminary data on their protective efficacy in animals. In this report, to advance the DISC vaccines to the marketplace, we investigated different parameters of these DISC vaccines. First, we demonstrated the genetic stabilities of these vaccine strains and also the complementing cell line. Subsequently, the optimal storage conditions of vaccines, including additives, temperature and desiccation were determined and their protective efficacies in animals confirmed. Further, to test if mixtures of different vaccine strains could be tolerated, we tested cocktails of DISC vaccines in combinations of three or six different serotypes in sheep and cattle, the two natural hosts of BTV. Groups of sheep vaccinated with a cocktail of six different vaccines were completely protected from challenge with individual virulent serotypes, both in early challenge or after five months' challenge without any clinical disease. There was no interference in protection between the different vaccines. Protection was also achieved in cattle with a mixture of three vaccine strains, albeit at a lesser level than sheep. Our data support and validate the suitability of these virus strains as the next generation vaccines for BTV.
Original languageEnglish
Article numbere01892-16
JournalJournal of Virology
Volume91
Issue number2
DOIs
Publication statusPublished - 2017

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Vaccines
vaccines
Viruses
viruses
Bluetongue virus
serotypes
Sheep
sheep
Vaccine Potency
Insect Vectors
Combined Vaccines
Desiccation
Livestock
livestock diseases
animals
insect vectors
genetic stability
cattle
Vaccination
crossover interference

Cite this

Celma, C. C., Stewart, M., Wernike, K., Eschbaumer, M., Gonzalez-Molleda, L., Breard, E., ... van Rijn, P. A. (2017). Replication-Deficient Particles: New Insights into the Next Generation of Bleutongue Virus Vaccines. Journal of Virology, 91(2), [e01892-16]. https://doi.org/10.1128/JVI.01892-16
Celma, Cristina C. ; Stewart, Meredith ; Wernike, Kerstine ; Eschbaumer, Michael ; Gonzalez-Molleda, Lorenzo ; Breard, Emmanuel ; Schulz, Claudia ; Hoffmann, Bernd ; Haegeman, Andy ; De Clercq, Kris ; van Rijn, P.A. / Replication-Deficient Particles: New Insights into the Next Generation of Bleutongue Virus Vaccines. In: Journal of Virology. 2017 ; Vol. 91, No. 2.
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abstract = "Bluetongue virus (BTV) is endemic in many parts of the world, often causing severe haemorrhagic disease in livestock. To date, at least 27 different serotypes have been recognized. Vaccination against all serotypes is necessary to protect susceptible animals and to prevent onward spread of the virus by insect vectors. In our previous studies, we generated replication-deficient (DISC) virus strains for a number of serotypes and reported preliminary data on their protective efficacy in animals. In this report, to advance the DISC vaccines to the marketplace, we investigated different parameters of these DISC vaccines. First, we demonstrated the genetic stabilities of these vaccine strains and also the complementing cell line. Subsequently, the optimal storage conditions of vaccines, including additives, temperature and desiccation were determined and their protective efficacies in animals confirmed. Further, to test if mixtures of different vaccine strains could be tolerated, we tested cocktails of DISC vaccines in combinations of three or six different serotypes in sheep and cattle, the two natural hosts of BTV. Groups of sheep vaccinated with a cocktail of six different vaccines were completely protected from challenge with individual virulent serotypes, both in early challenge or after five months' challenge without any clinical disease. There was no interference in protection between the different vaccines. Protection was also achieved in cattle with a mixture of three vaccine strains, albeit at a lesser level than sheep. Our data support and validate the suitability of these virus strains as the next generation vaccines for BTV.",
author = "Celma, {Cristina C.} and Meredith Stewart and Kerstine Wernike and Michael Eschbaumer and Lorenzo Gonzalez-Molleda and Emmanuel Breard and Claudia Schulz and Bernd Hoffmann and Andy Haegeman and {De Clercq}, Kris and {van Rijn}, P.A.",
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Celma, CC, Stewart, M, Wernike, K, Eschbaumer, M, Gonzalez-Molleda, L, Breard, E, Schulz, C, Hoffmann, B, Haegeman, A, De Clercq, K & van Rijn, PA 2017, 'Replication-Deficient Particles: New Insights into the Next Generation of Bleutongue Virus Vaccines', Journal of Virology, vol. 91, no. 2, e01892-16. https://doi.org/10.1128/JVI.01892-16

Replication-Deficient Particles: New Insights into the Next Generation of Bleutongue Virus Vaccines. / Celma, Cristina C.; Stewart, Meredith ; Wernike, Kerstine; Eschbaumer, Michael; Gonzalez-Molleda, Lorenzo; Breard, Emmanuel; Schulz, Claudia; Hoffmann, Bernd; Haegeman, Andy; De Clercq, Kris; van Rijn, P.A.

In: Journal of Virology, Vol. 91, No. 2, e01892-16, 2017.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Replication-Deficient Particles: New Insights into the Next Generation of Bleutongue Virus Vaccines

AU - Celma, Cristina C.

AU - Stewart, Meredith

AU - Wernike, Kerstine

AU - Eschbaumer, Michael

AU - Gonzalez-Molleda, Lorenzo

AU - Breard, Emmanuel

AU - Schulz, Claudia

AU - Hoffmann, Bernd

AU - Haegeman, Andy

AU - De Clercq, Kris

AU - van Rijn, P.A.

PY - 2017

Y1 - 2017

N2 - Bluetongue virus (BTV) is endemic in many parts of the world, often causing severe haemorrhagic disease in livestock. To date, at least 27 different serotypes have been recognized. Vaccination against all serotypes is necessary to protect susceptible animals and to prevent onward spread of the virus by insect vectors. In our previous studies, we generated replication-deficient (DISC) virus strains for a number of serotypes and reported preliminary data on their protective efficacy in animals. In this report, to advance the DISC vaccines to the marketplace, we investigated different parameters of these DISC vaccines. First, we demonstrated the genetic stabilities of these vaccine strains and also the complementing cell line. Subsequently, the optimal storage conditions of vaccines, including additives, temperature and desiccation were determined and their protective efficacies in animals confirmed. Further, to test if mixtures of different vaccine strains could be tolerated, we tested cocktails of DISC vaccines in combinations of three or six different serotypes in sheep and cattle, the two natural hosts of BTV. Groups of sheep vaccinated with a cocktail of six different vaccines were completely protected from challenge with individual virulent serotypes, both in early challenge or after five months' challenge without any clinical disease. There was no interference in protection between the different vaccines. Protection was also achieved in cattle with a mixture of three vaccine strains, albeit at a lesser level than sheep. Our data support and validate the suitability of these virus strains as the next generation vaccines for BTV.

AB - Bluetongue virus (BTV) is endemic in many parts of the world, often causing severe haemorrhagic disease in livestock. To date, at least 27 different serotypes have been recognized. Vaccination against all serotypes is necessary to protect susceptible animals and to prevent onward spread of the virus by insect vectors. In our previous studies, we generated replication-deficient (DISC) virus strains for a number of serotypes and reported preliminary data on their protective efficacy in animals. In this report, to advance the DISC vaccines to the marketplace, we investigated different parameters of these DISC vaccines. First, we demonstrated the genetic stabilities of these vaccine strains and also the complementing cell line. Subsequently, the optimal storage conditions of vaccines, including additives, temperature and desiccation were determined and their protective efficacies in animals confirmed. Further, to test if mixtures of different vaccine strains could be tolerated, we tested cocktails of DISC vaccines in combinations of three or six different serotypes in sheep and cattle, the two natural hosts of BTV. Groups of sheep vaccinated with a cocktail of six different vaccines were completely protected from challenge with individual virulent serotypes, both in early challenge or after five months' challenge without any clinical disease. There was no interference in protection between the different vaccines. Protection was also achieved in cattle with a mixture of three vaccine strains, albeit at a lesser level than sheep. Our data support and validate the suitability of these virus strains as the next generation vaccines for BTV.

U2 - 10.1128/JVI.01892-16

DO - 10.1128/JVI.01892-16

M3 - Article

VL - 91

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 2

M1 - e01892-16

ER -

Celma CC, Stewart M, Wernike K, Eschbaumer M, Gonzalez-Molleda L, Breard E et al. Replication-Deficient Particles: New Insights into the Next Generation of Bleutongue Virus Vaccines. Journal of Virology. 2017;91(2). e01892-16. https://doi.org/10.1128/JVI.01892-16