TY - JOUR
T1 - Renaissance of traditional DNA transfer strategies for improvement of industrial lactic acid bacteria
AU - Bron, Peter A.
AU - Marcelli, Barbara
AU - Mulder, Joyce
AU - van der Els, Simon
AU - Morawska, Luiza P.
AU - Kuipers, Oscar P.
AU - Kok, Jan
AU - Kleerebezem, Michiel
PY - 2019/4/1
Y1 - 2019/4/1
N2 - The ever-expanding genomic insight in natural diversity of lactic acid bacteria (LAB) has revived the industrial interest in traditional and natural genetic mobilization methodologies. Here, we review recent advances in horizontal gene transfer processes in LAB, including natural competence, conjugation, and phage transduction. In addition, we envision the possibilities for industrial strain improvement arising from the recent discoveries of molecular exchanges between bacteria through nanotubes and extracellular vesicles, as well as the constantly expanding genome editing possibilities using the CRISPR-Cas technology.
AB - The ever-expanding genomic insight in natural diversity of lactic acid bacteria (LAB) has revived the industrial interest in traditional and natural genetic mobilization methodologies. Here, we review recent advances in horizontal gene transfer processes in LAB, including natural competence, conjugation, and phage transduction. In addition, we envision the possibilities for industrial strain improvement arising from the recent discoveries of molecular exchanges between bacteria through nanotubes and extracellular vesicles, as well as the constantly expanding genome editing possibilities using the CRISPR-Cas technology.
U2 - 10.1016/j.copbio.2018.09.004
DO - 10.1016/j.copbio.2018.09.004
M3 - Article
AN - SCOPUS:85054564976
SN - 0958-1669
VL - 56
SP - 61
EP - 68
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -