Removing the dopaminergic inhibition with CRISPR-Cas9 to study the progression of sexual maturation in zebrafish

P.J.M. Jéhannet; A.A.C. de Wit; S. Brugman; Y. Zhang; J. Bogerd; A.P. Palstra

doi:10.3920/978-90-8686-940-4_490

Removing the dopaminergic inhibition with CRISPR-Cas9 to study the progression of sexual maturation in zebrafish

P.J.M. Jéhannet, A.A.C. de Wit, S. Brugman, Y. Zhang, J. Bogerd, A.P. Palstra

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic

Abstract

In fish, dopaminergic neurones originating from the preoptic area innerve the region of the pituitary gland that harbors gonadotropin cells. The innervation inhibits the release of gonadotropins that are essential for sexual maturation. The dopaminergic inhibition, mediated by dopaminergic receptors, is a major bottleneck in the propagation of many fish species in aquaculture. In this study, we aimed to knock-out one of the three dopamine receptors paralogue (drd2b) that mediates the inhibitory control of gonadotropins in zebrafish Danio rerio to study the progression of sexual maturation and potential occurrence of side-effects. Here, drd2b heterozygote or mosaic mutants were produced with an efficiency up to 73%. Knocking-out drd2b could be lethal since drd2b is involved in crucial processes during early ontogeny. If future studies confirm that inactivating drd2b is lethal, it may be useful to produce inducible and/or spatially restricted mutants to study sexual maturation.

Original language	English
Title of host publication	Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP)
Subtitle of host publication	Technical and species orientated innovations in animal breeding, and contribution of genetics to solving societal challenges
Editors	R.F. Veerkamp, Y. de Haas
Place of Publication	Wageningen
Publisher	Wageningen Academic Publishers
Pages	2036-2039
ISBN (Electronic)	9789086869404
DOIs	https://doi.org/10.3920/978-90-8686-940-4_490
Publication status	Published - 2022
Event	World Congress on Genetics Applied to Livestock Production: WCGALP 2022 - Rotterdam, Netherlands Duration: 3 Jul 2022 → 8 Jul 2022

Conference/symposium

Conference/symposium	World Congress on Genetics Applied to Livestock Production: WCGALP 2022
Country/Territory	Netherlands
City	Rotterdam
Period	3/07/22 → 8/07/22

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10.3920/978-90-8686-940-4_490Licence: CC BY

https://edepot.wur.nl/586589Licence: CC BY

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Jéhannet, P. J. M., de Wit, A. A. C., Brugman, S., Zhang, Y., Bogerd, J., & Palstra, A. P. (2022). Removing the dopaminergic inhibition with CRISPR-Cas9 to study the progression of sexual maturation in zebrafish. In R. F. Veerkamp, & Y. de Haas (Eds.), Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP): Technical and species orientated innovations in animal breeding, and contribution of genetics to solving societal challenges (pp. 2036-2039). Article 490 Wageningen Academic Publishers. https://doi.org/10.3920/978-90-8686-940-4_490

Jéhannet, P.J.M. ; de Wit, A.A.C. ; Brugman, S. et al. / Removing the dopaminergic inhibition with CRISPR-Cas9 to study the progression of sexual maturation in zebrafish. Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP): Technical and species orientated innovations in animal breeding, and contribution of genetics to solving societal challenges. editor / R.F. Veerkamp ; Y. de Haas. Wageningen : Wageningen Academic Publishers, 2022. pp. 2036-2039

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title = "Removing the dopaminergic inhibition with CRISPR-Cas9 to study the progression of sexual maturation in zebrafish",

abstract = "In fish, dopaminergic neurones originating from the preoptic area innerve the region of the pituitary gland that harbors gonadotropin cells. The innervation inhibits the release of gonadotropins that are essential for sexual maturation. The dopaminergic inhibition, mediated by dopaminergic receptors, is a major bottleneck in the propagation of many fish species in aquaculture. In this study, we aimed to knock-out one of the three dopamine receptors paralogue (drd2b) that mediates the inhibitory control of gonadotropins in zebrafish Danio rerio to study the progression of sexual maturation and potential occurrence of side-effects. Here, drd2b heterozygote or mosaic mutants were produced with an efficiency up to 73%. Knocking-out drd2b could be lethal since drd2b is involved in crucial processes during early ontogeny. If future studies confirm that inactivating drd2b is lethal, it may be useful to produce inducible and/or spatially restricted mutants to study sexual maturation.",

author = "P.J.M. J{\'e}hannet and {de Wit}, A.A.C. and S. Brugman and Y. Zhang and J. Bogerd and A.P. Palstra",

year = "2022",

doi = "10.3920/978-90-8686-940-4_490",

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Jéhannet, PJM, de Wit, AAC , Brugman, S, Zhang, Y, Bogerd, J & Palstra, AP 2022, Removing the dopaminergic inhibition with CRISPR-Cas9 to study the progression of sexual maturation in zebrafish. in RF Veerkamp & Y de Haas (eds), Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP): Technical and species orientated innovations in animal breeding, and contribution of genetics to solving societal challenges., 490, Wageningen Academic Publishers, Wageningen, pp. 2036-2039, World Congress on Genetics Applied to Livestock Production: WCGALP 2022, Rotterdam, Netherlands, 3/07/22. https://doi.org/10.3920/978-90-8686-940-4_490

Removing the dopaminergic inhibition with CRISPR-Cas9 to study the progression of sexual maturation in zebrafish. / Jéhannet, P.J.M.; de Wit, A.A.C.; Brugman, S. et al.
Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP): Technical and species orientated innovations in animal breeding, and contribution of genetics to solving societal challenges. ed. / R.F. Veerkamp; Y. de Haas. Wageningen: Wageningen Academic Publishers, 2022. p. 2036-2039 490.

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic

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AU - Zhang, Y.

AU - Bogerd, J.

AU - Palstra, A.P.

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AB - In fish, dopaminergic neurones originating from the preoptic area innerve the region of the pituitary gland that harbors gonadotropin cells. The innervation inhibits the release of gonadotropins that are essential for sexual maturation. The dopaminergic inhibition, mediated by dopaminergic receptors, is a major bottleneck in the propagation of many fish species in aquaculture. In this study, we aimed to knock-out one of the three dopamine receptors paralogue (drd2b) that mediates the inhibitory control of gonadotropins in zebrafish Danio rerio to study the progression of sexual maturation and potential occurrence of side-effects. Here, drd2b heterozygote or mosaic mutants were produced with an efficiency up to 73%. Knocking-out drd2b could be lethal since drd2b is involved in crucial processes during early ontogeny. If future studies confirm that inactivating drd2b is lethal, it may be useful to produce inducible and/or spatially restricted mutants to study sexual maturation.

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A2 - Veerkamp, R.F.

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ER -

Jéhannet PJM, de Wit AAC , Brugman S, Zhang Y, Bogerd J, Palstra AP. Removing the dopaminergic inhibition with CRISPR-Cas9 to study the progression of sexual maturation in zebrafish. In Veerkamp RF, de Haas Y, editors, Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP): Technical and species orientated innovations in animal breeding, and contribution of genetics to solving societal challenges. Wageningen: Wageningen Academic Publishers. 2022. p. 2036-2039. 490 doi: 10.3920/978-90-8686-940-4_490

Removing the dopaminergic inhibition with CRISPR-Cas9 to study the progression of sexual maturation in zebrafish

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