Removing the dopaminergic inhibition with CRISPR-Cas9 to study the progression of sexual maturation in zebrafish

P.J.M. Jéhannet, A.A.C. de Wit, S. Brugman, Y. Zhang, J. Bogerd, A.P. Palstra

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademic

Abstract

In fish, dopaminergic neurones originating from the preoptic area innerve the region of the pituitary gland that harbors gonadotropin cells. The innervation inhibits the release of gonadotropins that are essential for sexual maturation. The dopaminergic inhibition, mediated by dopaminergic receptors, is a major bottleneck in the propagation of many fish species in aquaculture. In this study, we aimed to knock-out one of the three dopamine receptors paralogue (drd2b) that mediates the inhibitory control of gonadotropins in zebrafish Danio rerio to study the progression of sexual maturation and potential occurrence of side-effects. Here, drd2b heterozygote or mosaic mutants were produced with an efficiency up to 73%. Knocking-out drd2b could be lethal since drd2b is involved in crucial processes during early ontogeny. If future studies confirm that inactivating drd2b is lethal, it may be useful to produce inducible and/or spatially restricted mutants to study sexual maturation.
Original languageEnglish
Title of host publicationProceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP)
Subtitle of host publicationTechnical and species orientated innovations in animal breeding, and contribution of genetics to solving societal challenges
EditorsR.F. Veerkamp, Y. de Haas
Place of PublicationWageningen
PublisherWageningen Academic Publishers
Pages2036-2039
ISBN (Electronic)9789086869404
DOIs
Publication statusPublished - 2022
EventWorld Congress on Genetics Applied to Livestock Production: WCGALP 2022 - Rotterdam, Netherlands
Duration: 3 Jul 20228 Jul 2022

Conference

ConferenceWorld Congress on Genetics Applied to Livestock Production: WCGALP 2022
Country/TerritoryNetherlands
CityRotterdam
Period3/07/228/07/22

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