Relationship between soil properties and the bias of N2O reduction by acetylene inhibition technique for analyzing soil denitrification potential

S.P. Qin, H.J. Yuan, W.X. Dong, C.S. Hu, O. Oenema, Y.M. Zhang

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The acetylene inhibition technique (AIT) has been widely used to measure soil denitrification potential (SDP), but has been criticized also for underestimating the actual SDP due to limitations of the AIT. Possible effects of soil properties on the bias of the AIT-derived SDP have not been thoroughly investigated yet. The study presented here therefore aimed at quantifying the relationships between soil texture and nutrient contents and the bias of AIT-derived SDP. A total of 26 soils with a wide range of clay and nutrient contents were incubated according to the standard procedure of AIT for assaying SDP. Incubation flasks were made anaerobic by gas substitution with pure helium (99.999%). Changes in the N-2 and N2O concentrations in the He headspace were measured using gas chromatography. The emission rates of N2O and N-2 were calculated to quantify the bias of the AIT-derived SDP (expressed as the percentage of the actual SDP that was not accounted for by AIT). The results showed that the bias ranged from 8 to 98%. The bias was negatively correlated (P <0.05) with the clay, silt, organic matter and nutrient contents of the soils. These results indicate that the bias of the AIT-derived SDP was higher in low-fertility soils than in fertile soils. We recommend that soil properties are taken into account when interpreting results of AIT-derived SDP values. (C) 2013 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)182-187
JournalSoil Biology and Biochemistry
Volume66
DOIs
Publication statusPublished - 2013

Fingerprint

Denitrification
Acetylene
acetylene
denitrification
soil properties
soil property
Soil
soil
methodology
nutrient content
clay
Food
helium
soil texture
soil nutrient
soil fertility
headspace analysis
soil nutrients
Helium
silt

Keywords

  • nitrous-oxide reduction
  • pseudomonas-aeruginosa
  • cultures
  • catchment
  • oxidation
  • sediments
  • nitrate
  • manure
  • assay
  • no

Cite this

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title = "Relationship between soil properties and the bias of N2O reduction by acetylene inhibition technique for analyzing soil denitrification potential",
abstract = "The acetylene inhibition technique (AIT) has been widely used to measure soil denitrification potential (SDP), but has been criticized also for underestimating the actual SDP due to limitations of the AIT. Possible effects of soil properties on the bias of the AIT-derived SDP have not been thoroughly investigated yet. The study presented here therefore aimed at quantifying the relationships between soil texture and nutrient contents and the bias of AIT-derived SDP. A total of 26 soils with a wide range of clay and nutrient contents were incubated according to the standard procedure of AIT for assaying SDP. Incubation flasks were made anaerobic by gas substitution with pure helium (99.999{\%}). Changes in the N-2 and N2O concentrations in the He headspace were measured using gas chromatography. The emission rates of N2O and N-2 were calculated to quantify the bias of the AIT-derived SDP (expressed as the percentage of the actual SDP that was not accounted for by AIT). The results showed that the bias ranged from 8 to 98{\%}. The bias was negatively correlated (P <0.05) with the clay, silt, organic matter and nutrient contents of the soils. These results indicate that the bias of the AIT-derived SDP was higher in low-fertility soils than in fertile soils. We recommend that soil properties are taken into account when interpreting results of AIT-derived SDP values. (C) 2013 Elsevier Ltd. All rights reserved.",
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Relationship between soil properties and the bias of N2O reduction by acetylene inhibition technique for analyzing soil denitrification potential. / Qin, S.P.; Yuan, H.J.; Dong, W.X.; Hu, C.S.; Oenema, O.; Zhang, Y.M.

In: Soil Biology and Biochemistry, Vol. 66, 2013, p. 182-187.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Relationship between soil properties and the bias of N2O reduction by acetylene inhibition technique for analyzing soil denitrification potential

AU - Qin, S.P.

AU - Yuan, H.J.

AU - Dong, W.X.

AU - Hu, C.S.

AU - Oenema, O.

AU - Zhang, Y.M.

N1 - WOS:000325665400022

PY - 2013

Y1 - 2013

N2 - The acetylene inhibition technique (AIT) has been widely used to measure soil denitrification potential (SDP), but has been criticized also for underestimating the actual SDP due to limitations of the AIT. Possible effects of soil properties on the bias of the AIT-derived SDP have not been thoroughly investigated yet. The study presented here therefore aimed at quantifying the relationships between soil texture and nutrient contents and the bias of AIT-derived SDP. A total of 26 soils with a wide range of clay and nutrient contents were incubated according to the standard procedure of AIT for assaying SDP. Incubation flasks were made anaerobic by gas substitution with pure helium (99.999%). Changes in the N-2 and N2O concentrations in the He headspace were measured using gas chromatography. The emission rates of N2O and N-2 were calculated to quantify the bias of the AIT-derived SDP (expressed as the percentage of the actual SDP that was not accounted for by AIT). The results showed that the bias ranged from 8 to 98%. The bias was negatively correlated (P <0.05) with the clay, silt, organic matter and nutrient contents of the soils. These results indicate that the bias of the AIT-derived SDP was higher in low-fertility soils than in fertile soils. We recommend that soil properties are taken into account when interpreting results of AIT-derived SDP values. (C) 2013 Elsevier Ltd. All rights reserved.

AB - The acetylene inhibition technique (AIT) has been widely used to measure soil denitrification potential (SDP), but has been criticized also for underestimating the actual SDP due to limitations of the AIT. Possible effects of soil properties on the bias of the AIT-derived SDP have not been thoroughly investigated yet. The study presented here therefore aimed at quantifying the relationships between soil texture and nutrient contents and the bias of AIT-derived SDP. A total of 26 soils with a wide range of clay and nutrient contents were incubated according to the standard procedure of AIT for assaying SDP. Incubation flasks were made anaerobic by gas substitution with pure helium (99.999%). Changes in the N-2 and N2O concentrations in the He headspace were measured using gas chromatography. The emission rates of N2O and N-2 were calculated to quantify the bias of the AIT-derived SDP (expressed as the percentage of the actual SDP that was not accounted for by AIT). The results showed that the bias ranged from 8 to 98%. The bias was negatively correlated (P <0.05) with the clay, silt, organic matter and nutrient contents of the soils. These results indicate that the bias of the AIT-derived SDP was higher in low-fertility soils than in fertile soils. We recommend that soil properties are taken into account when interpreting results of AIT-derived SDP values. (C) 2013 Elsevier Ltd. All rights reserved.

KW - nitrous-oxide reduction

KW - pseudomonas-aeruginosa

KW - cultures

KW - catchment

KW - oxidation

KW - sediments

KW - nitrate

KW - manure

KW - assay

KW - no

U2 - 10.1016/j.soilbio.2013.07.016

DO - 10.1016/j.soilbio.2013.07.016

M3 - Article

VL - 66

SP - 182

EP - 187

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

ER -