Regeneration, vernalization and flowering in Lunaria annua L. in vivo and in vitro

R.L.M. Pierik

    Research output: Thesisinternal PhD, WU


    Lunaria annua L. is a species with a juvenile phase and requiring cold for flowering. An annual flowering mutant was obtained with ethyl methane sulphonate. Experiments with shoot and leaf cuttings showed that juvenility was not a character of the whole plant. The juvenile phase was shortened by regeneration of cotyledon cuttings of very young plants.

    The effectiveness of vernalization in leaf cuttings closely depended on regeneration of buds before or during vernalization.

    Rejuvenation through regeneration is identical with juvenility. Flower induction in vitro in petiole segments of vegetative plants was effective, but only when buds were formed before or during vernalization. Cell divisions were considered to be a prerequisite to reach a certain stage of regeneration. Only from that stage could the low temperature act.

    Flower bud initiation in vitro in explants from the main axes of flowering Lunaria plants was influenced by different plant factors and by almost all environmental factors normally affecting growth. The disappearance of the flowering state was considered to be not directly due to meiosis or fertilization, but to plant regeneration.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • Wageningen University
    • Wellensiek, S.J., Promotor
    Award date21 Jun 1967
    Place of PublicationWageningen
    Publication statusPublished - 1967


    • brassicaceae
    • growth
    • plant development
    • ornamental plants
    • methodology
    • biological techniques
    • experiments
    • equipment
    • cum laude


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